Seroatlas · Human Serome Atlas

MIS12

Protein MIS12 homolog

Also known as: hMIS12, KNTC2AP, MGC2488, MIS12_HUMAN, MTW1

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9H081
Gene: MIS12
Ensembl: ENSG00000167842
Chromosome: 17
Canonical length: 205 aa
Protein class: Predicted intracellular proteins

OverviewNCBI Gene

Involved in attachment of mitotic spindle microtubules to kinetochore and kinetochore assembly. Located in kinetochore and nucleus. Part of MIS12/MIND type complex. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

205 residues, UniProt reviewed canonical sequence.

>Q9H081|MIS12
     1  MSVDPMTYEA QFFGFTPQTC MLRIYIAFQD YLFEVMQAVE QVILKKLDGI PDCDISPVQI
    61  RKCTEKFLCF MKGHFDNLFS KMEQLFLQLI LRIPSNILLP EDKCKETPYS EEDFQHLQKE
   121  IEQLQEKYKT ELCTKQALLA ELEEQKIVQA KLKQTLTFFD ELHNVGRDHG TSDFRESLVS
   181  LVQNSRKLQN IRDNVEKESK RLKIS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MIS12 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.44
Highest tissue expression: 30 nTPM

Expression across tissuesHPA

Tissue

epididymis: 30 nTPM
retina: 26 nTPM
thymus: 24 nTPM
lymph node: 20 nTPM
tonsil: 20 nTPM
thyroid gland: 18 nTPM

Single-cell type

late spermatids: 425 nCPM
epididymal principal cells: 254 nCPM
early spermatids: 120 nCPM
late primary spermatocytes: 95 nCPM
epididymal basal cells: 58 nCPM
cone photoreceptor cells: 50 nCPM

Immune cell

NK-cell: 16 nTPM
MAIT T-cell: 16 nTPM
naive CD4 T-cell: 16 nTPM
naive CD8 T-cell: 15 nTPM
gdT-cell: 15 nTPM
memory CD8 T-cell: 14 nTPM

Brain region

white matter: 24 nTPM
medulla oblongata: 18 nTPM
basal ganglia: 18 nTPM
midbrain: 16 nTPM
pons: 16 nTPM
cerebral cortex: 16 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.07
gnomAD pLI: 0.15
gnomAD missense Z: 0.64
DepMap mean gene effect: -1.12
DepMap dependency class: common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Cellular components

Protein domainsUniProt · Pfam · InterPro

Centromere protein Mis12
Mis12 protein

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of MIS12 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads MIS12 as an antibody target. Whether an autoantibody or antibody against MIS12 could matter depends on whether native MIS12 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

MIS12 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label MIS12 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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