Seroatlas · Human Serome Atlas

NHSL2

NHS-like protein 2

Also known as: NHSL2_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q5HYW2
Gene: NHSL2
Ensembl: ENSG00000204131
Chromosome: X
Canonical length: 1225 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoplasm,Plasma membrane,Cell Junctions

OverviewNCBI Gene

Predicted to enable calcium ion binding activity. Predicted to be involved in cell differentiation. Predicted to be active in cytoplasm. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

1225 residues, UniProt reviewed canonical sequence.

>Q5HYW2|NHSL2
     1  MPFYRRTVVP QRLCPRNPPQ QLAELRDVSH LAALSLLRQL ADLCGHSLAL LEDLEGHLLA
    61  LGRRTDSLYR RTVRLRRRLP CRLLGPEEDE EELAAANSGR ENATATAHSR SSWRQPVNVF
   121  LSSGRPPSVE ELLREAQLNL QSLLQEEYEE QYSEARLVGQ TFRSSDEATK PTPNPRPQSA
   181  RRLEFILMPT KRQLSEDETT TQGVRAPEAS LSLSTTADKQ TAWNSLFPLP ILEEKRWPQL
   241  CSTQSDIVPI NISGQQFDKH ASLRHSLFNT ETAVNPKSTL RRRRTIIGFS NFSQRDQGHS
   301  NSPAGSVAHS TTSDIRPSHS VPEGVHGRVA VGQDARFPSL TSPVLRTPSS EPDEPHQARS
   361  GPNPPGMESM GMVYSVPSSC NGPTESTFST SWKGDAFTYM TPSATSQSNQ VNENGKNPSC
   421  GNSWVSLNKV PPLVPKEAAT LLVARDNPAG CSGSAGYPER LIQQRHMPER PSKIGLLTSG
   481  TSRLETGPGG ASRFRERSLS VPTDSGTTDV DYDEEQKANE ACALPFASTS SEGSNSADNI
   541  ASLSAQQEAQ HRRQRSKSIS LRKAKKKPSP PTRSVSLVKD EPGLLPEGGS ALPKDQRPKS
   601  LCLSLEHQGH HSSHPDAQGH PAIPNHKDPE STQFSHHWYL TDWKSGDTYQ SLSSSSTATG
   661  TTVIECTQVQ GSSESLASPS TSRATTPSQL SIEVEAREIS SPGRPPGLMS PSSGYSSQSE
   721  TPTPTVSMSL TLGHLPPPSS SVRVRPVVPE RKSSLPPTSP MEKFPKSRLS FDLPLTSSPN
   781  LDLSGMSISI RSKTKVSRHH SETNFGVKLA QKTNPNQPIM PMVTQSDLRS VRLRSVSKSE
   841  PEDDIESPEY AEEPRAEEVF TLPERKTKPP VAEKPPVARR PPSLVHKPPS VPEEYALTSP
   901  TLAMPPRSSI QHARPLPQDS YTVVRKPKPS SFPDGRSPGE STAPSSLVFT PFASSSDAFF
   961  SGTQQPPQGS VEDEGPKVRV LPERISLQSQ EEAEKKKGKI PPPVPKKPSV LYLPLTSPTA
  1021  QMEAYVAEPR LPLSPIITLE EDTKCPATGD DLQSLGQRVT STPQADSERE ASPLGSSVEP
  1081  GTEEKSLISD KTAEWIAEDD DDVFVASRTT EDLFTVIHRS KRKLLGWKEP GEAFVGGRTS
  1141  SHSPIKNTAE SPISESTATA GSGSSANLDA GRNDDFKALL QKKGSKATPR SRPSAAELLK
  1201  TTNPLARRII AQFSKDYETT DNPST

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NHSL2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Unknown
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.69
Highest tissue expression: 11 nTPM

Expression across tissuesHPA

Tissue

cerebellum: 11 nTPM
smooth muscle: 6.4 nTPM
basal ganglia: 5.3 nTPM
colon: 4.7 nTPM
adipose tissue: 4.5 nTPM
seminal vesicle: 4.5 nTPM

Single-cell type

choroid plexus epithelial cells: 935 nCPM
pituicytes/fscs: 260 nCPM
neutrophils: 255 nCPM
fibro-adipogenic progenitors: 173 nCPM
mesothelial cells: 132 nCPM
epicardial cells: 116 nCPM

Immune cell

neutrophil: 16 nTPM
eosinophil: 14 nTPM
classical monocyte: 7.1 nTPM
basophil: 5 nTPM
intermediate monocyte: 4.7 nTPM
myeloid DC: 4.1 nTPM

Brain region

choroid plexus: 34 nTPM
cerebellum: 32 nTPM
basal ganglia: 28 nTPM
midbrain: 28 nTPM
amygdala: 24 nTPM
hypothalamus: 22 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.52
gnomAD pLI: 0.12
gnomAD missense Z: 0.74

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

cell differentiation

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Phosphoprotein

InteractionsUniProt · HPA

Protein binding partners of NHSL2 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads NHSL2 as an antibody target. Whether an autoantibody or antibody against NHSL2 could matter depends on whether native NHSL2 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

NHSL2 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label NHSL2 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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