NCK2
Cytoplasmic protein NCK2
Also known as: NCK2_HUMAN, NCKbeta
Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene
Protein identityUniProt · HPA
- UniProt accession
- O43639
- Gene
- NCK2
- Ensembl
- ENSG00000071051
- Chromosome
- 2
- Canonical length
- 380 aa
- Protein class
- Plasma proteins, Predicted intracellular proteins
OverviewNCBI Gene
This gene encodes a member of the NCK family of adaptor proteins. The protein contains three SH3 domains and one SH2 domain. The protein has no known catalytic function but has been shown to bind and recruit various proteins involved in the regulation of receptor protein tyrosine kinases. It is through these regulatory activities that this protein is believed to be involved in cytoskeletal reorganization. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
Canonical amino-acid sequenceUniProt
380 residues, UniProt reviewed canonical sequence.
>O43639|NCK2
1 MTEEVIVIAK WDYTAQQDQE LDIKKNERLW LLDDSKTWWR VRNAANRTGY VPSNYVERKN
61 SLKKGSLVKN LKDTLGLGKT RRKTSARDAS PTPSTDAEYP ANGSGADRIY DLNIPAFVKF
121 AYVAEREDEL SLVKGSRVTV MEKCSDGWWR GSYNGQIGWF PSNYVLEEVD EAAAESPSFL
181 SLRKGASLSN GQGSRVLHVV QTLYPFSSVT EEELNFEKGE TMEVIEKPEN DPEWWKCKNA
241 RGQVGLVPKN YVVVLSDGPA LHPAHAPQIS YTGPSSSGRF AGREWYYGNV TRHQAECALN
301 ERGVEGDFLI RDSESSPSDF SVSLKASGKN KHFKVQLVDN VYCIGQRRFH TMDELVEHYK
361 KAPIFTSEHG EKLYLVRALQLocalizationUniProt · AlphaFold · HPA
Whether an antibody against NCK2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.
- Antibody reachability
- Intracellular
- Secreted
- No
- Transmembrane segments
- 0
- Mean surface accessibility (rSASA)
- 0.4
- Highest tissue expression
- 67 nTPM
Expression across tissuesHPA
Tissue
- esophagus: 67 nTPM
- duodenum: 51 nTPM
- skeletal muscle: 50 nTPM
- spleen: 49 nTPM
- small intestine: 49 nTPM
- vagina: 49 nTPM
Single-cell type
- platelets: 915 nCPM
- esophageal apical cells: 356 nCPM
- microglia: 298 nCPM
- neutrophils: 263 nCPM
- t-cells: 257 nCPM
- sertoli cells: 196 nCPM
Immune cell
- basophil: 26 nTPM
- T-reg: 25 nTPM
- naive CD4 T-cell: 14 nTPM
- memory CD4 T-cell: 13 nTPM
- eosinophil: 13 nTPM
- naive CD8 T-cell: 13 nTPM
Brain region
- cerebral cortex: 55 nTPM
- hippocampal formation: 53 nTPM
- cerebellum: 49 nTPM
- thalamus: 45 nTPM
- midbrain: 43 nTPM
- spinal cord: 43 nTPM
Genetic constraint and essentialitygnomAD · DepMap
Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.
- gnomAD LOEUF (loss-of-function intolerance)
- 0.54
- gnomAD pLI
- 0.46
- gnomAD missense Z
- 2.45
- DepMap mean gene effect
- 0.05
- DepMap dependency class
- none
Cancer expressionTCGA
Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).
OntologyGO
Biological processes
- actin filament organization
- cell migration
- dendritic spine development
- ephrin receptor signaling pathway
- epidermal growth factor receptor signaling pathway
- immunological synapse formation
- lamellipodium assembly
- negative regulation of cell population proliferation
- negative regulation of PERK-mediated unfolded protein response
- negative regulation of transcription by RNA polymerase II
- positive regulation of actin filament polymerization
- positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway
- positive regulation of T cell proliferation
- positive regulation of transcription by RNA polymerase II
- positive regulation of translation in response to endoplasmic reticulum stress
- regulation of translation initiation in response to endoplasmic reticulum stress
- signal complex assembly
- signal transduction
- T cell activation
Molecular functions
- cytoskeletal anchor activity
- phosphotyrosine residue binding
- protein-containing complex binding
- receptor tyrosine kinase binding
- scaffold protein binding
- signaling adaptor activity
- signaling receptor complex adaptor activity
Cellular components
Protein domainsUniProt · Pfam · InterPro
- SH2 domain
- SH3 domain
- Cytoplasmic protein NCK
- SH3-like domain superfamily
- SH2 domain superfamily
- Tyrosine-phosphorylated adapter molecule
- SH2 domain
- SH3 domain
- Variant SH3 domain
- Nck2, SH3 domain 1
- Nck2, SH3 domain 2
- Nck2, SH3 domain 3
- Nck2, SH2 domain
KeywordsUniProt
InteractionsUniProt · HPA
Protein binding partners of NCK2 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.
Antibody and autoantibody relevanceSeroatlas analysis
Seroatlas reads NCK2 as an antibody target. Whether an autoantibody or antibody against NCK2 could matter depends on whether native NCK2 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.
NCK2 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.
Annotation status
The present source text does not explicitly label NCK2 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.
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