Seroatlas · Human Serome Atlas

MAP4K1

Mitogen-activated protein kinase kinase kinase kinase 1

Also known as: HPK1, M4K1_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q92918
Gene: MAP4K1
Ensembl: ENSG00000104814
Chromosome: 19
Canonical length: 833 aa
Protein class: Enzymes, Predicted intracellular proteins
Subcellular location: Plasma membrane

OverviewNCBI Gene

Enables ATP binding activity and MAP kinase kinase kinase kinase activity. Involved in several processes, including JNK cascade; cellular response to phorbol 13-acetate 12-myristate; and protein phosphorylation. Located in membrane. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

833 residues, UniProt reviewed canonical sequence.

>Q92918|MAP4K1
     1  MDVVDPDIFN RDPRDHYDLL QRLGGGTYGE VFKARDKVSG DLVALKMVKM EPDDDVSTLQ
    61  KEILILKTCR HANIVAYHGS YLWLQKLWIC MEFCGAGSLQ DIYQVTGSLS ELQISYVCRE
   121  VLQGLAYLHS QKKIHRDIKG ANILINDAGE VRLADFGISA QIGATLARRL SFIGTPYWMA
   181  PEVAAVALKG GYNELCDIWS LGITAIELAE LQPPLFDVHP LRVLFLMTKS GYQPPRLKEK
   241  GKWSAAFHNF IKVTLTKSPK KRPSATKMLS HQLVSQPGLN RGLILDLLDK LKNPGKGPSI
   301  GDIEDEEPEL PPAIPRRIRS THRSSSLGIP DADCCRRHME FRKLRGMETR PPANTARLQP
   361  PRDLRSSSPR KQLSESSDDD YDDVDIPTPA EDTPPPLPPK PKFRSPSDEG PGSMGDDGQL
   421  SPGVLVRCAS GPPPNSPRPG PPPSTSSPHL TAHSEPSLWN PPSRELDKPP LLPPKKEKMK
   481  RKGCALLVKL FNGCPLRIHS TAAWTHPSTK DQHLLLGAEE GIFILNRNDQ EATLEMLFPS
   541  RTTWVYSINN VLMSLSGKTP HLYSHSILGL LERKETRAGN PIAHISPHRL LARKNMVSTK
   601  IQDTKGCRAC CVAEGASSGG PFLCGALETS VVLLQWYQPM NKFLLVRQVL FPLPTPLSVF
   661  ALLTGPGSEL PAVCIGVSPG RPGKSVLFHT VRFGALSCWL GEMSTEHRGP VQVTQVEEDM
   721  VMVLMDGSVK LVTPEGSPVR GLRTPEIPMT EAVEAVAMVG GQLQAFWKHG VQVWALGSDQ
   781  LLQELRDPTL TFRLLGSPRL ECSGTISPHC NLLLPGSSNS PASASRVAGI TGL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MAP4K1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.37
Highest tissue expression: 55 nTPM

Expression across tissuesHPA

Tissue

spleen: 55 nTPM
lymph node: 30 nTPM
small intestine: 28 nTPM
tonsil: 28 nTPM
retina: 15 nTPM
appendix: 14 nTPM

Single-cell type

oocytes: 74 nCPM
rod photoreceptor cells: 45 nCPM
pdcs: 32 nCPM
b-cells: 31 nCPM
cdc: 31 nCPM
t-cells: 30 nCPM

Immune cell

myeloid DC: 24 nTPM
memory B-cell: 23 nTPM
T-reg: 22 nTPM
plasmacytoid DC: 22 nTPM
memory CD8 T-cell: 20 nTPM
gdT-cell: 19 nTPM

Brain region

medulla oblongata: 1.5 nTPM
white matter: 1.5 nTPM
pons: 1.4 nTPM
thalamus: 1.4 nTPM
hypothalamus: 1.2 nTPM
midbrain: 1.2 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.3
gnomAD pLI: 0.99
gnomAD missense Z: 3.13
DepMap mean gene effect: 0.01
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of MAP4K1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads MAP4K1 as an antibody target. Whether an autoantibody or antibody against MAP4K1 could matter depends on whether native MAP4K1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

MAP4K1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label MAP4K1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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