Seroatlas · Human Serome Atlas

UFL1

E3 UFM1-protein ligase 1

Also known as: KIAA0776, Maxer, NLBP, RCAD, UFL1_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
O94874
Gene
UFL1
Ensembl
ENSG00000014123
Chromosome
6
Canonical length
794 aa
Protein class
Predicted intracellular proteins
Subcellular location
Endoplasmic reticulum

OverviewNCBI Gene

Enables UFM1 ligase activity and protein kinase binding activity. Involved in several processes, including positive regulation of reticulophagy; regulation of intracellular signal transduction; and regulation of primary metabolic process. Acts upstream of or within several processes, including positive regulation of cell population proliferation; regulation of proteasomal ubiquitin-dependent protein catabolic process; and response to endoplasmic reticulum stress. Located in cytoplasm; nucleus; and site of double-strand break. Part of protein-containing complex. Is active in endoplasmic reticulum membrane and mitochondrial outer membrane. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

794 residues, UniProt reviewed canonical sequence.

>O94874|UFL1
     1  MADAWEEIRR LAADFQRAQF AEATQRLSER NCIEIVNKLI AQKQLEVVHT LDGKEYITPA
    61  QISKEMRDEL HVRGGRVNIV DLQQVINVDL IHIENRIGDI IKSEKHVQLV LGQLIDENYL
   121  DRLAEEVNDK LQESGQVTIS ELCKTYDLPG NFLTQALTQR LGRIISGHID LDNRGVIFTE
   181  AFVARHKARI RGLFSAITRP TAVNSLISKY GFQEQLLYSV LEELVNSGRL RGTVVGGRQD
   241  KAVFVPDIYS RTQSTWVDSF FRQNGYLEFD ALSRLGIPDA VSYIKKRYKT TQLLFLKAAC
   301  VGQGLVDQVE ASVEEAISSG TWVDIAPLLP TSLSVEDAAI LLQQVMRAFS KQASTVVFSD
   361  TVVVSEKFIN DCTELFRELM HQKAEKEMKN NPVHLITEED LKQISTLESV STSKKDKKDE
   421  RRRKATEGSG SMRGGGGGNA REYKIKKVKK KGRKDDDSDD ESQSSHTGKK KPEISFMFQD
   481  EIEDFLRKHI QDAPEEFISE LAEYLIKPLN KTYLEVVRSV FMSSTTSASG TGRKRTIKDL
   541  QEEVSNLYNN IRLFEKGMKF FADDTQAALT KHLLKSVCTD ITNLIFNFLA SDLMMAVDDP
   601  AAITSEIRKK ILSKLSEETK VALTKLHNSL NEKSIEDFIS CLDSAAEACD IMVKRGDKKR
   661  ERQILFQHRQ ALAEQLKVTE DPALILHLTS VLLFQFSTHS MLHAPGRCVP QIIAFLNSKI
   721  PEDQHALLVK YQGLVVKQLV SQSKKTGQGD YPLNNELDKE QEDVASTTRK ELQELSSSIK
   781  DLVLKSRKSS VTEE

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against UFL1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Other membrane
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.35
Highest tissue expression
29 nTPM

Expression across tissuesHPA

Tissue

  • retina: 29 nTPM
  • liver: 28 nTPM
  • thyroid gland: 26 nTPM
  • pancreas: 24 nTPM
  • parathyroid gland: 24 nTPM
  • prostate: 23 nTPM

Single-cell type

  • corticotrophs: 144 nCPM
  • syncytiotrophoblasts: 144 nCPM
  • pancreatic acinar cells: 135 nCPM
  • esophageal apical cells: 130 nCPM
  • plasma cells: 126 nCPM
  • epididymal principal cells: 121 nCPM

Immune cell

  • NK-cell: 13 nTPM
  • naive CD4 T-cell: 11 nTPM
  • T-reg: 11 nTPM
  • plasmacytoid DC: 11 nTPM
  • non-classical monocyte: 10 nTPM
  • myeloid DC: 10 nTPM

Brain region

  • white matter: 40 nTPM
  • medulla oblongata: 32 nTPM
  • basal ganglia: 27 nTPM
  • spinal cord: 26 nTPM
  • hypothalamus: 26 nTPM
  • cerebellum: 25 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.84
gnomAD pLI
0
gnomAD missense Z
0.19
DepMap mean gene effect
-0.37
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

  • E3 UFM1-protein ligase 1
  • E3 UFM1-protein ligase 1-like, N-terminal
  • E3 UFM1-protein ligase 1-like domain
  • E3 UFM1-protein ligase-like, C-terminal domain
  • E3 UFM1-protein ligase 1
  • E3 UFM1-protein ligase 1-like domain
  • E3 UFM1-protein ligase 1 C-terminal domain
  • Winged helix-turn-helix domain 5 in UFL1 protein

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of UFL1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads UFL1 as an antibody target. Whether an autoantibody or antibody against UFL1 could matter depends on whether native UFL1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

UFL1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label UFL1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/UFL1. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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