CDK9
Cyclin-dependent kinase 9
Also known as: C-2k, CDC2L4, CDK9_HUMAN, PITALRE, TAK
Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene
Protein identityUniProt · HPA
- UniProt accession
- P50750
- Gene
- CDK9
- Ensembl
- ENSG00000136807
- Chromosome
- 9
- Canonical length
- 372 aa
- Protein class
- Disease related genes, Enzymes, Plasma proteins, Potential drug targets, Predicted intracellular proteins
- Subcellular location
- Nucleoplasm,Cytoplasmic bodies
OverviewNCBI Gene
The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS. [provided by RefSeq, Jul 2008]
Canonical amino-acid sequenceUniProt
372 residues, UniProt reviewed canonical sequence.
>P50750|CDK9
1 MAKQYDSVEC PFCDEVSKYE KLAKIGQGTF GEVFKARHRK TGQKVALKKV LMENEKEGFP
61 ITALREIKIL QLLKHENVVN LIEICRTKAS PYNRCKGSIY LVFDFCEHDL AGLLSNVLVK
121 FTLSEIKRVM QMLLNGLYYI HRNKILHRDM KAANVLITRD GVLKLADFGL ARAFSLAKNS
181 QPNRYTNRVV TLWYRPPELL LGERDYGPPI DLWGAGCIMA EMWTRSPIMQ GNTEQHQLAL
241 ISQLCGSITP EVWPNVDNYE LYEKLELVKG QKRKVKDRLK AYVRDPYALD LIDKLLVLDP
301 AQRIDSDDAL NHDFFWSDPM PSDLKGMLST HLTSMFEYLA PPRRKGSQIT QQSTNQSRNP
361 ATTNQTEFER VFLocalizationUniProt · AlphaFold · HPA
Whether an antibody against CDK9 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.
- Antibody reachability
- Intracellular
- Secreted
- No
- Transmembrane segments
- 0
- Mean surface accessibility (rSASA)
- 0.3
- Highest tissue expression
- 85 nTPM
Expression across tissuesHPA
Tissue
- ovary: 85 nTPM
- cerebellum: 67 nTPM
- pituitary gland: 65 nTPM
- spleen: 63 nTPM
- fallopian tube: 62 nTPM
- liver: 62 nTPM
Single-cell type
- bergmann glia: 66 nCPM
- syncytiotrophoblasts: 64 nCPM
- ependymal cells: 63 nCPM
- esophageal apical cells: 55 nCPM
- podocytes: 50 nCPM
- astrocytes: 49 nCPM
Immune cell
- NK-cell: 23 nTPM
- gdT-cell: 10 nTPM
- MAIT T-cell: 10 nTPM
- plasmacytoid DC: 10 nTPM
- memory CD8 T-cell: 8.3 nTPM
- naive CD8 T-cell: 7.9 nTPM
Brain region
- cerebral cortex: 73 nTPM
- hippocampal formation: 72 nTPM
- midbrain: 60 nTPM
- medulla oblongata: 60 nTPM
- white matter: 59 nTPM
- amygdala: 59 nTPM
Genetic constraint and essentialitygnomAD · DepMap
Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.
- gnomAD LOEUF (loss-of-function intolerance)
- 0.95
- gnomAD pLI
- 0
- gnomAD missense Z
- 2.14
- DepMap mean gene effect
- -1.45
- DepMap dependency class
- pan
Cancer expressionTCGA
Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).
OntologyGO
Biological processes
- cell population proliferation
- cellular response to cytokine stimulus
- DNA repair
- host-mediated activation of viral transcription
- negative regulation of protein localization to chromatin
- nucleus localization
- positive regulation of protein localization to chromatin
- positive regulation of transcription by RNA polymerase II
- positive regulation of transcription elongation by RNA polymerase II
- protein phosphorylation
- regulation of cell cycle
- regulation of DNA repair
- regulation of mRNA 3'-end processing
- regulation of muscle cell differentiation
- replication fork processing
- transcription by RNA polymerase II
- transcription elongation by RNA polymerase II
- transcription elongation-coupled chromatin remodeling
- transcription initiation at RNA polymerase II promoter
Molecular functions
- 7SK snRNA binding
- ATP binding
- chromatin binding
- cyclin-dependent protein serine/threonine kinase activity
- DNA binding
- kinase activity
- protein kinase activity
- protein kinase binding
- protein serine kinase activity
- protein serine/threonine kinase activity
- RNA polymerase II cis-regulatory region sequence-specific DNA binding
- RNA polymerase II CTD heptapeptide repeat kinase activity
- transcription coactivator binding
- transcription elongation factor activity
- histone H1-4S187 kinase activity
Cellular components
Protein domainsUniProt · Pfam · InterPro
KeywordsUniProt
InteractionsUniProt · HPA
Protein binding partners of CDK9 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.
Antibody and autoantibody relevanceSeroatlas analysis
Seroatlas reads CDK9 as an antibody target. Whether an autoantibody or antibody against CDK9 could matter depends on whether native CDK9 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.
CDK9 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.
Annotation status
The present source text does not explicitly label CDK9 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.
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