Seroatlas · Human Serome Atlas

TOPBP1

DNA topoisomerase 2-binding protein 1

Also known as: Dpb11, KIAA0259, TOP2BP1, TOPB1_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q92547
Gene
TOPBP1
Ensembl
ENSG00000163781
Chromosome
3
Canonical length
1522 aa
Protein class
Predicted intracellular proteins
Subcellular location
Nucleoplasm,Nuclear bodies,Plasma membrane

OverviewNCBI Gene

This gene encodes a binding protein which interacts with the C-terminal region of topoisomerase II beta. This interaction suggests a supportive role for this protein in the catalytic reactions of topoisomerase II beta through transient breakages of DNA strands. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

1522 residues, UniProt reviewed canonical sequence.

>Q92547|TOPBP1
     1  MSRNDKEPFF VKFLKSSDNS KCFFKALESI KEFQSEEYLQ IITEEEALKI KENDRSLYIC
    61  DPFSGVVFDH LKKLGCRIVG PQVVIFCMHH QRCVPRAEHP VYNMVMSDVT ISCTSLEKEK
   121  REEVHKYVQM MGGRVYRDLN VSVTHLIAGE VGSKKYLVAA NLKKPILLPS WIKTLWEKSQ
   181  EKKITRYTDI NMEDFKCPIF LGCIICVTGL CGLDRKEVQQ LTVKHGGQYM GQLKMNECTH
   241  LIVQEPKGQK YECAKRWNVH CVTTQWFFDS IEKGFCQDES IYKTEPRPEA KTMPNSSTPT
   301  SQINTIDSRT LSDVSNISNI NASCVSESIC NSLNSKLEPT LENLENLDVS AFQAPEDLLD
   361  GCRIYLCGFS GRKLDKLRRL INSGGGVRFN QLNEDVTHVI VGDYDDELKQ FWNKSAHRPH
   421  VVGAKWLLEC FSKGYMLSEE PYIHANYQPV EIPVSHKPES KAALLKKKNS SFSKKDFAPS
   481  EKHEQADEDL LSQYENGSST VVEAKTSEAR PFNDSTHAEP LNDSTHISLQ EENQSSVSHC
   541  VPDVSTITEE GLFSQKSFLV LGFSNENESN IANIIKENAG KIMSLLSRTV ADYAVVPLLG
   601  CEVEATVGEV VTNTWLVTCI DYQTLFDPKS NPLFTPVPVM TGMTPLEDCV ISFSQCAGAE
   661  KESLTFLANL LGASVQEYFV RKSNAKKGMF ASTHLILKER GGSKYEAAKK WNLPAVTIAW
   721  LLETARTGKR ADESHFLIEN STKEERSLET EITNGINLNS DTAEHPGTRL QTHRKTVVTP
   781  LDMNRFQSKA FRAVVSQHAR QVAASPAVGQ PLQKEPSLHL DTPSKFLSKD KLFKPSFDVK
   841  DALAALETPG RPSQQKRKPS TPLSEVIVKN LQLALANSSR NAVALSASPQ LKEAQSEKEE
   901  APKPLHKVVV CVSKKLSKKQ SELNGIAASL GADYRWSFDE TVTHFIYQGR PNDTNREYKS
   961  VKERGVHIVS EHWLLDCAQE CKHLPESLYP HTYNPKMSLD ISAVQDGRLC NSRLLSAVSS
  1021  TKDDEPDPLI LEENDVDNMA TNNKESAPSN GSGKNDSKGV LTQTLEMREN FQKQLQEIMS
  1081  ATSIVKPQGQ RTSLSRSGCN SASSTPDSTR SARSGRSRVL EALRQSRQTV PDVNTEPSQN
  1141  EQIIWDDPTA REERARLASN LQWPSCPTQY SELQVDIQNL EDSPFQKPLH DSEIAKQAVC
  1201  DPGNIRVTEA PKHPISEELE TPIKDSHLIP TPQAPSIAFP LANPPVAPHP REKIITIEET
  1261  HEELKKQYIF QLSSLNPQER IDYCHLIEKL GGLVIEKQCF DPTCTHIVVG HPLRNEKYLA
  1321  SVAAGKWVLH RSYLEACRTA GHFVQEEDYE WGSSSILDVL TGINVQQRRL ALAAMRWRKK
  1381  IQQRQESGIV EGAFSGWKVI LHVDQSREAG FKRLLQSGGA KVLPGHSVPL FKEATHLFSD
  1441  LNKLKPDDSG VNIAEAAAQN VYCLRTEYIA DYLMQESPPH VENYCLPEAI SFIQNNKELG
  1501  TGLSQKRKAP TEKNKIKRPR VH

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against TOPBP1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.44
Highest tissue expression
31 nTPM

Expression across tissuesHPA

Tissue

  • bone marrow: 31 nTPM
  • thymus: 27 nTPM
  • testis: 21 nTPM
  • lymph node: 18 nTPM
  • tonsil: 17 nTPM
  • appendix: 12 nTPM

Single-cell type

  • neutrophil progenitors: 204 nCPM
  • monocyte progenitors: 170 nCPM
  • late spermatids: 156 nCPM
  • erythrocyte progenitors: 129 nCPM
  • megakaryocyte progenitors: 105 nCPM
  • early primary spermatocytes: 87 nCPM

Immune cell

  • eosinophil: 2.2 nTPM
  • basophil: 1.8 nTPM
  • T-reg: 1.5 nTPM
  • MAIT T-cell: 1 nTPM
  • NK-cell: 1 nTPM
  • plasmacytoid DC: 1 nTPM

Brain region

  • cerebellum: 13 nTPM
  • cerebral cortex: 12 nTPM
  • white matter: 12 nTPM
  • basal ganglia: 11 nTPM
  • medulla oblongata: 11 nTPM
  • pons: 11 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.09
gnomAD pLI
1
gnomAD missense Z
1.97
DepMap mean gene effect
-1.42
DepMap dependency class
pan

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 11% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of TOPBP1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads TOPBP1 as an antibody target. Whether an autoantibody or antibody against TOPBP1 could matter depends on whether native TOPBP1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

TOPBP1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label TOPBP1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/TOPBP1. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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