IFI16

Protein identityUniProt · HPA

UniProt accession

Q16666

Gene

IFI16

Ensembl

ENSG00000163565

Chromosome

1

Canonical length

785 aa

Protein class

Plasma proteins, Predicted intracellular proteins

Subcellular location

Nucleoplasm,Nucleoli,Cytosol

Quaternary structure

Homooligomer

OverviewNCBI Gene

This gene encodes a member of the HIN-200 (hematopoietic interferon-inducible nuclear antigens with 200 amino acid repeats) family of cytokines. The encoded protein contains domains involved in DNA binding, transcriptional regulation, and protein-protein interactions. The protein localizes to the nucleoplasm and nucleoli, and interacts with p53 and retinoblastoma-1. It modulates p53 function, and inhibits cell growth in the Ras/Raf signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2011]

Canonical amino-acid sequenceUniProt

785 residues, UniProt reviewed canonical sequence.

>Q16666|IFI16
     1  MGKKYKNIVL LKGLEVINDY HFRMVKSLLS NDLKLNLKMR EEYDKIQIAD LMEEKFRGDA
    61  GLGKLIKIFE DIPTLEDLAE TLKKEKLKVK GPALSRKRKK EVDATSPAPS TSSTVKTEGA
   121  EATPGAQKRK KSTKEKAGPK GSKVSEEQTQ PPSPAGAGMS TAMGRSPSPK TSLSAPPNSS
   181  STENPKTVAK CQVTPRRNVL QKRPVIVKVL STTKPFEYET PEMEKKIMFH ATVATQTQFF
   241  HVKVLNTSLK EKFNGKKIII ISDYLEYDSL LEVNEESTVS EAGPNQTFEV PNKIINRAKE
   301  TLKIDILHKQ ASGNIVYGVF MLHKKTVNQK TTIYEIQDDR GKMDVVGTGQ CHNIPCEEGD
   361  KLQLFCFRLR KKNQMSKLIS EMHSFIQIKK KTNPRNNDPK SMKLPQEQRQ LPYPSEASTT
   421  FPESHLRTPQ MPPTTPSSSF FTKKSEDTIS KMNDFMRMQI LKEGSHFPGP FMTSIGPAES
   481  HPHTPQMPPS TPSSSFLTTK SEDTISKMND FMRMQILKEG SHFPGPFMTS IGPAESHPHT
   541  PQMPPSTPSS SFLTTLKPRL KTEPEEVSIE DSAQSDLKEV MVLNATESFV YEPKEQKKMF
   601  HATVATENEV FRVKVFNIDL KEKFTPKKII AIANYVCRNG FLEVYPFTLV ADVNADRNME
   661  IPKGLIRSAS VTPKINQLCS QTKGSFVNGV FEVHKKNVRG EFTYYEIQDN TGKMEVVVHG
   721  RLTTINCEEG DKLKLTCFEL APKSGNTGEL RSVIHSHIKV IKTRKNKKDI LNPDSSMETS
   781  PDFFF

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against IFI16 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.45

Highest tissue expression

131 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about IFI16.

Disease | AutoantibodyPubMed

Conditions in which antibodies against IFI16 are reported. Each links to that disease's full target list.

• Sjogren's Syndrome 5
• Lupus Erythematosus, Systemic 4
• Scleroderma, Systemic 4

ReferencesPubMed · IEDB

Publications for IFI16 from three distinct lines of evidence, kept separate because they answer different questions: whether antibodies are directed at the protein, whether a B-cell epitope has been mapped on it, and whether a T-cell epitope has. Each is labelled with its source.

Sources: PubMed — antigen-level antibody evidence from a custom retrieval. Records matching a controlled set of autoantibody terms (the MeSH descriptors Autoantibodies and Autoantigens, with title and abstract term variants) were obtained through NCBI E-utilities, and their titles and abstracts parsed for constructions that direct an antibody at a named protein rather than for co-occurrence. Captured names were resolved against UniProt nomenclature and each antigen adjudicated individually against the source text. Bibliographic records from PubMed and MeSH, U.S. National Library of Medicine; citation metrics from NIH iCite (Hutchins et al., PLoS Biology 2016). Titles link to PubMed; abstracts are not reproduced here. The NLM does not endorse this analysis.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.03

gnomAD pLI

0

gnomAD missense Z

0.57

DepMap mean gene effect

0.01

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• activation of innate immune response
• autophagy
• cellular response to glucose starvation
• cellular response to interferon-beta
• cellular response to ionizing radiation
• defense response to virus
• inflammatory response
• innate immune response
• intrinsic apoptotic signaling pathway by p53 class mediator
• intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
• monocyte differentiation
• myeloid cell differentiation
• negative regulation of AIM2 inflammasome complex assembly
• negative regulation of DNA binding
• negative regulation of DNA-templated transcription
• negative regulation of gene expression, epigenetic
• negative regulation of innate immune response
• negative regulation of transcription by RNA polymerase II
• negative regulation of viral genome replication
• positive regulation of cytokine production
• positive regulation of interleukin-1 beta production
• positive regulation of transcription by RNA polymerase II
• regulation of autophagy
• regulation of inflammatory response

Molecular functions

• double-stranded DNA binding
• identical protein binding
• RNA binding
• transcription factor binding

Cellular components

• cytoplasm
• cytosol
• membrane
• nuclear speck
• nucleolus
• nucleoplasm
• nucleus

Protein domainsUniProt · Pfam · InterPro

• DAPIN domain
• HIN-200/IF120x
• Death-like domain superfamily
• Nucleic acid-binding, OB-fold
• HIN-200 family
• PAAD/DAPIN/Pyrin domain
• HIN-200/IF120x domain

KeywordsUniProt

• Acetylation
• Activator
• Apoptosis
• Autophagy
• Cytoplasm
• DNA-binding
• Immunity
• Inflammatory response
• Innate immunity
• Isopeptide bond
• Nucleus
• Phosphoprotein
• Repeat
• Repressor
• Transcription
• Transcription regulation
• Ubl conjugation

InteractionsUniProt · HPA

Protein binding partners of IFI16 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• AIM2
• BRCA1
• CASP1
• E2F1
• MTA1
• PYCARD
• PYDC5
• RB1
• SP1
• STING1
• TMEM173
• TP53
• AR