CEBPA
CCAAT/enhancer-binding protein alpha
Also known as: C/EBP-alpha, CEBP, CEBPA_HUMAN
Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene
Protein identityUniProt · HPA
- UniProt accession
- P49715
- Gene
- CEBPA
- Ensembl
- ENSG00000245848
- Chromosome
- 19
- Canonical length
- 358 aa
- Protein class
- Cancer-related genes, Disease related genes, Human disease related genes, Predicted intracellular proteins, Transcription factors
- Subcellular location
- Nucleoplasm,Vesicles
- Quaternary structure
- Homodimer
OverviewNCBI Gene
This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain and recognizes the CCAAT motif in the promoters of target genes. The encoded protein functions in homodimers and also heterodimers with CCAAT/enhancer-binding proteins beta and gamma. Activity of this protein can modulate the expression of genes involved in cell cycle regulation as well as in body weight homeostasis. Mutation of this gene is associated with acute myeloid leukemia. The use of alternative in-frame non-AUG (GUG) and AUG start codons results in protein isoforms with different lengths. Differential translation initiation is mediated by an out-of-frame, upstream open reading frame which is located between the GUG and the first AUG start codons. [provided by RefSeq, Dec 2013]
Canonical amino-acid sequenceUniProt
358 residues, UniProt reviewed canonical sequence.
>P49715|CEBPA
1 MESADFYEAE PRPPMSSHLQ SPPHAPSSAA FGFPRGAGPA QPPAPPAAPE PLGGICEHET
61 SIDISAYIDP AAFNDEFLAD LFQHSRQQEK AKAAVGPTGG GGGGDFDYPG APAGPGGAVM
121 PGGAHGPPPG YGCAAAGYLD GRLEPLYERV GAPALRPLVI KQEPREEDEA KQLALAGLFP
181 YQPPPPPPPS HPHPHPPPAH LAAPHLQFQI AHCGQTTMHL QPGHPTPPPT PVPSPHPAPA
241 LGAAGLPGPG SALKGLGAAH PDLRASGGSG AGKAKKSVDK NSNEYRVRRE RNNIAVRKSR
301 DKAKQRNVET QQKVLELTSD NDRLRKRVEQ LSRELDTLRG IFRQLPESSL VKAMGNCALocalizationUniProt · AlphaFold · HPA
Whether an antibody against CEBPA can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.
- Antibody reachability
- Intracellular
- Secreted
- No
- Transmembrane segments
- 0
- Mean surface accessibility (rSASA)
- 0.66
- Highest tissue expression
- 198 nTPM
Expression across tissuesHPA
Tissue
- liver: 198 nTPM
- skin: 115 nTPM
- adipose tissue: 93 nTPM
- breast: 68 nTPM
- parathyroid gland: 25 nTPM
- lung: 24 nTPM
Single-cell type
- syncytiotrophoblasts: 278 nCPM
- suprabasal keratinocytes: 129 nCPM
- hofbauer cells: 114 nCPM
- hepatocytes: 111 nCPM
- microglia: 87 nCPM
- alveolar cells type 2: 84 nCPM
Immune cell
- basophil: 16 nTPM
- non-classical monocyte: 16 nTPM
- intermediate monocyte: 12 nTPM
- classical monocyte: 7.1 nTPM
- myeloid DC: 5.1 nTPM
- neutrophil: 2.5 nTPM
Brain region
- white matter: 59 nTPM
- medulla oblongata: 43 nTPM
- spinal cord: 40 nTPM
- thalamus: 37 nTPM
- midbrain: 33 nTPM
- pons: 29 nTPM
DiseaseUniProt · ClinVar · IEDB · PubMed
Four sources answering four different questions about CEBPA.
Disease | AllUniProt
Conditions CEBPA is implicated in, by any mechanism.
- Leukemia, acute myelogenous (AML) MIM:601626
Disease | GeneticClinVar
66 pathogenic / likely-pathogenic of 1,280 ClinVar records.
Conditions with pathogenic or likely-pathogenic variants.
Genetic constraint and essentialitygnomAD · DepMap
Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.
- gnomAD LOEUF (loss-of-function intolerance)
- 1.18
- gnomAD pLI
- 0.55
- gnomAD missense Z
- 0.57
- DepMap mean gene effect
- -0.08
- DepMap dependency class
- selective
Cancer expressionTCGA
Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).
OntologyGO
Biological processes
- acute-phase response
- animal organ regeneration
- brown fat cell differentiation
- cellular response to lithium ion
- cellular response to tumor necrosis factor
- cellular response to xenobiotic stimulus
- cholesterol metabolic process
- cytokine-mediated signaling pathway
- DNA-templated transcription
- embryonic placenta development
- energy homeostasis
- epithelial cell maturation
- fat cell differentiation
- generation of precursor metabolites and energy
- glucose homeostasis
- granulocyte differentiation
- hematopoietic stem cell proliferation
- inner ear development
- integrated stress response signaling
- interleukin-6-mediated signaling pathway
- lipid homeostasis
- liver development
- lung development
- macrophage differentiation
- memory
- mitochondrion organization
- myeloid cell differentiation
- negative regulation of cell cycle
- negative regulation of cell population proliferation
- negative regulation of DNA-templated transcription
- negative regulation of hematopoietic stem cell proliferation
- negative regulation of transcription by RNA polymerase II
- Notch signaling pathway
- osteoblast development
- positive regulation of DNA-templated transcription initiation
- positive regulation of fat cell differentiation
- positive regulation of gene expression
- positive regulation of inflammatory response
- positive regulation of macrophage activation
- positive regulation of osteoblast differentiation
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process
- positive regulation of transcription by RNA polymerase II
- regulation of cell cycle
- regulation of DNA-templated transcription
- regulation of transcription by RNA polymerase II
- response to dexamethasone
- response to phenylpropanoid
- response to vitamin B2
- transcription by RNA polymerase I
- urea cycle
- white fat cell differentiation
- white fat cell proliferation
Molecular functions
- chromatin DNA binding
- DNA binding
- DNA-binding transcription activator activity, RNA polymerase II-specific
- DNA-binding transcription factor activity
- DNA-binding transcription factor activity, RNA polymerase II-specific
- histone deacetylase binding
- HMG box domain binding
- identical protein binding
- kinase binding
- protein heterodimerization activity
- protein homodimerization activity
- protein-containing complex binding
- RNA polymerase II cis-regulatory region sequence-specific DNA binding
- RNA polymerase II-specific DNA-binding transcription factor binding
- STAT family protein binding
- transcription cis-regulatory region binding
- RNA polymerase I transcription regulatory region sequence-specific DNA binding
Cellular components
Protein domainsUniProt · Pfam · InterPro
KeywordsUniProt
InteractionsUniProt · HPA
Protein binding partners of CEBPA in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.
Antibody and autoantibody relevanceSeroatlas analysis
Seroatlas reads CEBPA as an antibody target. Whether an autoantibody or antibody against CEBPA could matter depends on whether native CEBPA is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.
CEBPA is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.
Annotation status
The present source text does not explicitly label CEBPA as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.
Loading the interactive Seroatlas protein explorer...