Seroatlas · Human Serome Atlas

EEF1A1

Elongation factor 1-alpha 1

Also known as: EE1A1, EEF1A, EF1A, EF1A1, EF1A1_HUMAN, EF1alpha1, LENG7

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: P68104
Gene: EEF1A1
Ensembl: ENSG00000156508
Chromosome: 6
Canonical length: 462 aa
Protein class: Cancer-related genes, Metabolic proteins, Plasma proteins, Predicted intracellular proteins
Subcellular location: Cytosol

OverviewNCBI Gene

This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

462 residues, UniProt reviewed canonical sequence.

>P68104|EEF1A1
     1  MGKEKTHINI VVIGHVDSGK STTTGHLIYK CGGIDKRTIE KFEKEAAEMG KGSFKYAWVL
    61  DKLKAERERG ITIDISLWKF ETSKYYVTII DAPGHRDFIK NMITGTSQAD CAVLIVAAGV
   121  GEFEAGISKN GQTREHALLA YTLGVKQLIV GVNKMDSTEP PYSQKRYEEI VKEVSTYIKK
   181  IGYNPDTVAF VPISGWNGDN MLEPSANMPW FKGWKVTRKD GNASGTTLLE ALDCILPPTR
   241  PTDKPLRLPL QDVYKIGGIG TVPVGRVETG VLKPGMVVTF APVNVTTEVK SVEMHHEALS
   301  EALPGDNVGF NVKNVSVKDV RRGNVAGDSK NDPPMEAAGF TAQVIILNHP GQISAGYAPV
   361  LDCHTAHIAC KFAELKEKID RRSGKKLEDG PKFLKSGDAA IVDMVPGKPM CVESFSDYPP
   421  LGRFAVRDMR QTVAVGVIKA VDKKAAGAGK VTKSAQKAQK AK

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against EEF1A1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Cell surface
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.23
Highest tissue expression: 11,521 nTPM

Expression across tissuesHPA

Tissue

ovary: 11,521 nTPM
tonsil: 10,582 nTPM
bone marrow: 9,894 nTPM
breast: 9,820 nTPM
pancreas: 9,814 nTPM
fallopian tube: 8,503 nTPM

Single-cell type

enteric stem cells: 5,148 nCPM
epididymal efferent duct absorptive cells: 4,828 nCPM
paneth cells: 4,011 nCPM
enteric transient amplifying cells: 3,990 nCPM
epididymal basal cells: 3,858 nCPM
epididymal principal cells: 3,304 nCPM

Immune cell

total PBMC: 26,787 nTPM
MAIT T-cell: 14,267 nTPM
naive CD4 T-cell: 13,952 nTPM
naive CD8 T-cell: 12,085 nTPM
memory CD4 T-cell: 12,039 nTPM
memory B-cell: 10,614 nTPM

Brain region

spinal cord: 2,942 nTPM
choroid plexus: 2,720 nTPM
white matter: 2,719 nTPM
medulla oblongata: 2,551 nTPM
thalamus: 2,322 nTPM
hypothalamus: 2,302 nTPM

ReferencesPubMed · IEDB

Publications for EEF1A1 from three distinct lines of evidence, kept separate because they answer different questions: whether antibodies are directed at the protein, whether a B-cell epitope has been mapped on it, and whether a T-cell epitope has. Each is labelled with its source.

Reference: AutoantibodyPubMed

2 publications

Cloning and expression of a novel human antibody-antigen pair associated with Felty's syndrome.
2000 · Proc Natl Acad Sci U S A · RCR 1.2 · 50 citations
The Level of Autoantibodies Targeting Eukaryote Translation Elongation Factor 1 α1 and Ubiquitin-Conjugating Enzyme 2L3 in Nondiabetic Young Adults.
2016 · Diabetes Metab J · RCR 0.3 · 8 citations

Sources: PubMed — antigen-level antibody evidence from a custom retrieval. Records matching a controlled set of autoantibody terms (the MeSH descriptors Autoantibodies and Autoantigens, with title and abstract term variants) were obtained through NCBI E-utilities, and their titles and abstracts parsed for constructions that direct an antibody at a named protein rather than for co-occurrence. Captured names were resolved against UniProt nomenclature and each antigen adjudicated individually against the source text. Bibliographic records from PubMed and MeSH, U.S. National Library of Medicine; citation metrics from NIH iCite (Hutchins et al., PLoS Biology 2016). Titles link to PubMed; abstracts are not reproduced here. The NLM does not endorse this analysis.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.29
gnomAD pLI: 0.98
gnomAD missense Z: 3.72
DepMap mean gene effect: -2.09
DepMap dependency class: pan

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of EEF1A1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads EEF1A1 as an antibody target. Whether an autoantibody or antibody against EEF1A1 could matter depends on whether native EEF1A1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

EEF1A1 is annotated at the cell surface, where native EEF1A1 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Source-annotated serology context

The source annotations explicitly mention antibody, autoantibody, autoantigen, or autoimmune context. This is biological context, not study-specific reactivity.

This isoform is identified as an autoantigen in 66% of patients with Felty syndrome.

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