Seroatlas · Human Serome Atlas

CNOT1

CCR4-NOT transcription complex subunit 1

Also known as: AD-005, CDC39, CNOT1_HUMAN, KIAA1007, NOT1, NOT1H

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: A5YKK6
Gene: CNOT1
Ensembl: ENSG00000125107
Chromosome: 16
Canonical length: 2376 aa
Protein class: Disease related genes, Human disease related genes, Plasma proteins, Predicted intracellular proteins, Predicted membrane proteins
Subcellular location: Cytosol

OverviewNCBI Gene

Enables armadillo repeat domain binding activity; molecular adaptor activity; and nuclear receptor binding activity. Contributes to poly(A)-specific ribonuclease activity. Involved in several processes, including negative regulation of intracellular signal transduction; positive regulation of cytoplasmic mRNA processing body assembly; and regulation of gene expression. Located in P-body and cytosol. Part of CCR4-NOT complex. Implicated in Vissers-Bodmer syndrome and holoprosencephaly 12. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

2376 residues, UniProt reviewed canonical sequence.

>A5YKK6|CNOT1
     1  MNLDSLSLAL SQISYLVDNL TKKNYRASQQ EIQHIVNRHG PEADRHLLRC LFSHVDFSGD
    61  GKSSGKDFHQ TQFLIQECAL LITKPNFIST LSYAIDNPLH YQKSLKPAPH LFAQLSKVLK
   121  LSKVQEVIFG LALLNSSSSD LRGFAAQFIK QKLPDLLRSY IDADVSGNQE GGFQDIAIEV
   181  LHLLLSHLLF GQKGAFGVGQ EQIDAFLKTL RRDFPQERCP VVLAPLLYPE KRDILMDRIL
   241  PDSGGVAKTM MESSLADFMQ EVGYGFCASI EECRNIIVQF GVREVTAAQV ARVLGMMART
   301  HSGLTDGIPL QSISAPGSGI WSDGKDKSDG AQAHTWNVEV LIDVLKELNP SLNFKEVTYE
   361  LDHPGFQIRD SKGLHNVVYG IQRGLGMEVF PVDLIYRPWK HAEGQLSFIQ HSLINPEIFC
   421  FADYPCHTVA TDILKAPPED DNREIATWKS LDLIESLLRL AEVGQYEQVK QLFSFPIKHC
   481  PDMLVLALLQ INTSWHTLRH ELISTLMPIF LGNHPNSAII LHYAWHGQGQ SPSIRQLIMH
   541  AMAEWYMRGE QYDQAKLSRI LDVAQDLKAL SMLLNGTPFA FVIDLAALAS RREYLKLDKW
   601  LTDKIREHGE PFIQACMTFL KRRCPSILGG LAPEKDQPKS AQLPPETLAT MLACLQACAG
   661  SVSQELSETI LTMVANCSNV MNKARQPPPG VMPKGRPPSA SSLDAISPVQ IDPLAGMTSL
   721  SIGGSAAPHT QSMQGFPPNL GSAFSTPQSP AKAFPPLSTP NQTTAFSGIG GLSSQLPVGG
   781  LGTGSLTGIG TGALGLPAVN NDPFVQRKLG TSGLNQPTFQ QSKMKPSDLS QVWPEANQHF
   841  SKEIDDEANS YFQRIYNHPP HPTMSVDEVL EMLQRFKDST IKREREVFNC MLRNLFEEYR
   901  FFPQYPDKEL HITACLFGGI IEKGLVTYMA LGLALRYVLE ALRKPFGSKM YYFGIAALDR
   961  FKNRLKDYPQ YCQHLASISH FMQFPHHLQE YIEYGQQSRD PPVKMQGSIT TPGSIALAQA
  1021  QAQAQVPAKA PLAGQVSTMV TTSTTTTVAK TVTVTRPTGV SFKKDVPPSI NTTNIDTLLV
  1081  ATDQTERIVE PPENIQEKIA FIFNNLSQSN MTQKVEELKE TVKEEFMPWV SQYLVMKRVS
  1141  IEPNFHSLYS NFLDTLKNPE FNKMVLNETY RNIKVLLTSD KAAANFSDRS LLKNLGHWLG
  1201  MITLAKNKPI LHTDLDVKSL LLEAYVKGQQ ELLYVVPFVA KVLESSIRSV VFRPPNPWTM
  1261  AIMNVLAELH QEHDLKLNLK FEIEVLCKNL ALDINELKPG NLLKDKDRLK NLDEQLSAPK
  1321  KDVKQPEELP PITTTTTSTT PATNTTCTAT VPPQPQYSYH DINVYSLAGL APHITLNPTI
  1381  PLFQAHPQLK QCVRQAIERA VQELVHPVVD RSIKIAMTTC EQIVRKDFAL DSEESRMRIA
  1441  AHHMMRNLTA GMAMITCREP LLMSISTNLK NSFASALRTA SPQQREMMDQ AAAQLAQDNC
  1501  ELACCFIQKT AVEKAGPEMD KRLATEFELR KHARQEGRRY CDPVVLTYQA ERMPEQIRLK
  1561  VGGVDPKQLA VYEEFARNVP GFLPTNDLSQ PTGFLAQPMK QAWATDDVAQ IYDKCITELE
  1621  QHLHAIPPTL AMNPQAQALR SLLEVVVLSR NSRDAIAALG LLQKAVEGLL DATSGADADL
  1681  LLRYRECHLL VLKALQDGRA YGSPWCNKQI TRCLIECRDE YKYNVEAVEL LIRNHLVNMQ
  1741  QYDLHLAQSM ENGLNYMAVA FAMQLVKILL VDERSVAHVT EADLFHTIET LMRINAHSRG
  1801  NAPEGLPQLM EVVRSNYEAM IDRAHGGPNF MMHSGISQAS EYDDPPGLRE KAEYLLREWV
  1861  NLYHSAAAGR DSTKAFSAFV GQMHQQGILK TDDLITRFFR LCTEMCVEIS YRAQAEQQHN
  1921  PAANPTMIRA KCYHNLDAFV RLIALLVKHS GEATNTVTKI NLLNKVLGIV VGVLLQDHDV
  1981  RQSEFQQLPY HRIFIMLLLE LNAPEHVLET INFQTLTAFC NTFHILRPTK APGFVYAWLE
  2041  LISHRIFIAR MLAHTPQQKG WPMYAQLLID LFKYLAPFLR NVELTKPMQI LYKGTLRVLL
  2101  VLLHDFPEFL CDYHYGFCDV IPPNCIQLRN LILSAFPRNM RLPDPFTPNL KVDMLSEINI
  2161  APRILTNFTG VMPPQFKKDL DSYLKTRSPV TFLSDLRSNL QVSNEPGNRY NLQLINALVL
  2221  YVGTQAIAHI HNKGSTPSMS TITHSAHMDI FQNLAVDLDT EGRYLFLNAI ANQLRYPNSH
  2281  THYFSCTMLY LFAEANTEAI QEQITRVLLE RLIVNRPHPW GLLITFIELI KNPAFKFWNH
  2341  EFVHCAPEIE KLFQSVAQCC MGQKQAQQVM EGTGAS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CNOT1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.33
Highest tissue expression: 101 nTPM

Expression across tissuesHPA

Tissue

testis: 101 nTPM
thymus: 71 nTPM
esophagus: 69 nTPM
tonsil: 65 nTPM
colon: 56 nTPM
parathyroid gland: 56 nTPM

Single-cell type

esophageal apical cells: 340 nCPM
neutrophils: 290 nCPM
cardiomyocytes: 286 nCPM
neutrophil progenitors: 256 nCPM
myonuclei: 221 nCPM
monocyte progenitors: 187 nCPM

Immune cell

non-classical monocyte: 23 nTPM
neutrophil: 19 nTPM
basophil: 18 nTPM
MAIT T-cell: 18 nTPM
intermediate monocyte: 16 nTPM
T-reg: 16 nTPM

Brain region

choroid plexus: 103 nTPM
cerebellum: 65 nTPM
white matter: 61 nTPM
cerebral cortex: 57 nTPM
medulla oblongata: 56 nTPM
thalamus: 56 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about CNOT1.

Disease | AllUniProt

Conditions CNOT1 is implicated in, by any mechanism.

Holoprosencephaly 12 with or without pancreatic agenesis (HPE12) MIM:618500
Vissers-Bodmer syndrome (VIBOS) MIM:619033

Disease | GeneticClinVar

47 pathogenic / likely-pathogenic of 953 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

Vissers-Bodmer syndrome
Holoprosencephaly 12 with or without pancreatic agenesis
Inborn genetic diseases
CNOT1-related disorder
Neurodevelopmental delay

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.04
gnomAD pLI: 1
gnomAD missense Z: 7.25
DepMap mean gene effect: -0.82
DepMap dependency class: common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

CCR4-Not complex component, Not1, C-terminal
CCR4-NOT transcription complex subunit 1, domain 4
CCR4-NOT transcription complex subunit 1, CAF1-binding domain
CCR4-NOT transcription complex subunit 1, TTP binding domain
CCR4-NOT transcription complex subunit 1, HEAT repeat
CCR4-NOT subunit 1, TTP binding domain superfamily
CCR4-NOT transcription complex subunit 1
CCR4-NOT transcription complex subunit 1, N-terminal domain
CCR4-NOT transcription complex subunit 1-like, NOT1 connector
CCR4-Not complex component, Not1
CCR4-Not complex, Not1 subunit, domain of unknown function DUF3819
CCR4-NOT transcription complex subunit 1 CAF1-binding domain
CCR4-NOT transcription complex subunit 1 TTP binding domain
CCR4-NOT transcription complex subunit 1 HEAT repeat
CCR4-NOT transcription complex subunit 1, N-terminal domain
CCR4-NOT transcription complex subunit 1, NOT1_connector

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of CNOT1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads CNOT1 as an antibody target. Whether an autoantibody or antibody against CNOT1 could matter depends on whether native CNOT1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

CNOT1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label CNOT1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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