Seroatlas · Human Serome Atlas

YTHDF1

YTH domain-containing family protein 1

Also known as: C20orf21, FLJ20391, YTHD1_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q9BYJ9
Gene
YTHDF1
Ensembl
ENSG00000149658
Chromosome
20
Canonical length
559 aa
Protein class
Predicted intracellular proteins

OverviewNCBI Gene

Enables N6-methyladenosine-containing RNA reader activity and ribosome binding activity. Involved in mRNA destabilization; positive regulation of translational initiation; and stress granule assembly. Located in P-body and cytoplasmic stress granule. Implicated in colorectal cancer. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

559 residues, UniProt reviewed canonical sequence.

>Q9BYJ9|YTHDF1
     1  MSATSVDTQR TKGQDNKVQN GSLHQKDTVH DNDFEPYLTG QSNQSNSYPS MSDPYLSSYY
    61  PPSIGFPYSL NEAPWSTAGD PPIPYLTTYG QLSNGDHHFM HDAVFGQPGG LGNNIYQHRF
   121  NFFPENPAFS AWGTSGSQGQ QTQSSAYGSS YTYPPSSLGG TVVDGQPGFH SDTLSKAPGM
   181  NSLEQGMVGL KIGDVSSSAV KTVGSVVSSV ALTGVLSGNG GTNVNMPVSK PTSWAAIASK
   241  PAKPQPKMKT KSGPVMGGGL PPPPIKHNMD IGTWDNKGPV PKAPVPQQAP SPQAAPQPQQ
   301  VAQPLPAQPP ALAQPQYQSP QQPPQTRWVA PRNRNAAFGQ SGGAGSDSNS PGNVQPNSAP
   361  SVESHPVLEK LKAAHSYNPK EFEWNLKSGR VFIIKSYSED DIHRSIKYSI WCSTEHGNKR
   421  LDSAFRCMSS KGPVYLLFSV NGSGHFCGVA EMKSPVDYGT SAGVWSQDKW KGKFDVQWIF
   481  VKDVPNNQLR HIRLENNDNK PVTNSRDTQE VPLEKAKQVL KIISSYKHTT SIFDDFAHYE
   541  KRQEEEEVVR KERQSRNKQ

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against YTHDF1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.57
Highest tissue expression
45 nTPM

Expression across tissuesHPA

Tissue

  • skeletal muscle: 45 nTPM
  • bone marrow: 39 nTPM
  • tongue: 33 nTPM
  • cerebellum: 32 nTPM
  • liver: 30 nTPM
  • pituitary gland: 28 nTPM

Single-cell type

  • hofbauer cells: 61 nCPM
  • early primary spermatocytes: 55 nCPM
  • gastric progenitor cells: 50 nCPM
  • breast lactating cells: 41 nCPM
  • somatotrophs: 41 nCPM
  • lactotrophs: 41 nCPM

Immune cell

  • MAIT T-cell: 3.7 nTPM
  • naive CD4 T-cell: 3.5 nTPM
  • eosinophil: 3.2 nTPM
  • T-reg: 3.2 nTPM
  • gdT-cell: 3.1 nTPM
  • memory CD4 T-cell: 3.1 nTPM

Brain region

  • medulla oblongata: 43 nTPM
  • midbrain: 39 nTPM
  • thalamus: 39 nTPM
  • hypothalamus: 38 nTPM
  • cerebral cortex: 37 nTPM
  • white matter: 37 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.24
gnomAD pLI
0.99
gnomAD missense Z
1.6
DepMap mean gene effect
0.15
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 21% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of YTHDF1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads YTHDF1 as an antibody target. Whether an autoantibody or antibody against YTHDF1 could matter depends on whether native YTHDF1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

YTHDF1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label YTHDF1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/YTHDF1. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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