Seroatlas · Human Serome Atlas

CNOT8

CCR4-NOT transcription complex subunit 8

Also known as: CAF1, CALIF, CNOT8_HUMAN, hCAF1, POP2

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9UFF9
Gene: CNOT8
Ensembl: ENSG00000155508
Chromosome: 5
Canonical length: 292 aa
Protein class: Enzymes, Plasma proteins, Predicted intracellular proteins

OverviewNCBI Gene

Enables poly(A)-specific ribonuclease activity. Involved in miRNA-mediated gene silencing by mRNA destabilization and positive regulation of cell population proliferation. Located in nucleus. Part of CCR4-NOT complex. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

292 residues, UniProt reviewed canonical sequence.

>Q9UFF9|CNOT8
     1  MPAALVENSQ VICEVWASNL EEEMRKIREI VLSYSYIAMD TEFPGVVVRP IGEFRSSIDY
    61  QYQLLRCNVD LLKIIQLGLT FTNEKGEYPS GINTWQFNFK FNLTEDMYSQ DSIDLLANSG
   121  LQFQKHEEEG IDTLHFAELL MTSGVVLCDN VKWLSFHSGY DFGYMVKLLT DSRLPEEEHE
   181  FFHILNLFFP SIYDVKYLMK SCKNLKGGLQ EVADQLDLQR IGRQHQAGSD SLLTGMAFFR
   241  MKELFFEDSI DDAKYCGRLY GLGTGVAQKQ NEDVDSAQEK MSILAIINNM QQ

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CNOT8 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.27
Highest tissue expression: 112 nTPM

Expression across tissuesHPA

Tissue

bone marrow: 112 nTPM
thymus: 65 nTPM
adipose tissue: 61 nTPM
lymph node: 55 nTPM
blood vessel: 53 nTPM
tonsil: 50 nTPM

Single-cell type

early spermatids: 130 nCPM
neutrophils: 125 nCPM
decidual stromal cells: 113 nCPM
late primary spermatocytes: 94 nCPM
alveolar cells type 2: 92 nCPM
neutrophil progenitors: 88 nCPM

Immune cell

basophil: 278 nTPM
eosinophil: 270 nTPM
total PBMC: 170 nTPM
myeloid DC: 133 nTPM
classical monocyte: 128 nTPM
neutrophil: 127 nTPM

Brain region

choroid plexus: 61 nTPM
thalamus: 45 nTPM
white matter: 45 nTPM
spinal cord: 44 nTPM
medulla oblongata: 42 nTPM
pons: 41 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.58
gnomAD pLI: 0.22
gnomAD missense Z: 2.16
DepMap mean gene effect: -0.04
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of CNOT8 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads CNOT8 as an antibody target. Whether an autoantibody or antibody against CNOT8 could matter depends on whether native CNOT8 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

CNOT8 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label CNOT8 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Loading the interactive Seroatlas protein explorer...