Seroatlas · Human Serome Atlas

CNOT4

CCR4-NOT transcription complex subunit 4

Also known as: CLONE243, CNOT4_HUMAN, NOT4, NOT4H

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
O95628
Gene
CNOT4
Ensembl
ENSG00000080802
Chromosome
7
Canonical length
575 aa
Protein class
Enzymes, Metabolic proteins, Predicted intracellular proteins, Transcription factors

OverviewNCBI Gene

The protein encoded by this gene is a subunit of the CCR4-NOT complex, a global transcriptional regulator. The encoded protein interacts with CNOT1 and has E3 ubiquitin ligase activity. Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2010]

Canonical amino-acid sequenceUniProt

575 residues, UniProt reviewed canonical sequence.

>O95628|CNOT4
     1  MSRSPDAKED PVECPLCMEP LEIDDINFFP CTCGYQICRF CWHRIRTDEN GLCPACRKPY
    61  PEDPAVYKPL SQEELQRIKN EKKQKQNERK QKISENRKHL ASVRVVQKNL VFVVGLSQRL
   121  ADPEVLKRPE YFGKFGKIHK VVINNSTSYA GSQGPSASAY VTYIRSEDAL RAIQCVNNVV
   181  VDGRTLKASL GTTKYCSYFL KNMQCPKPDC MYLHELGDEA ASFTKEEMQA GKHQEYEQKL
   241  LQELYKLNPN FLQLSTGSVD KNKNKVTPLQ RYDTPIDKPS DSLSIGNGDN SQQISNSDTP
   301  SPPPGLSKSN PVIPISSSNH SARSPFEGAV TESQSLFSDN FRHPNPIPSG LPPFPSSPQT
   361  SSDWPTAPEP QSLFTSETIP VSSSTDWQAA FGFGSSKQPE DDLGFDPFDV TRKALADLIE
   421  KELSVQDQPS LSPTSLQNSS SHTTTAKGPG SGFLHPAAAT NANSLNSTFS VLPQRFPQFQ
   481  QHRAVYNSFS FPGQAARYPW MAFPRNSIMH LNHTANPTSN SNFLDLNLPP QHNTGLGGIP
   541  VAGEEEVKVS TMPLSTSSHS LQQGQQPTSL HTTVA

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CNOT4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.56
Highest tissue expression
21 nTPM

Expression across tissuesHPA

Tissue

  • skeletal muscle: 21 nTPM
  • tongue: 21 nTPM
  • testis: 16 nTPM
  • retina: 14 nTPM
  • ovary: 13 nTPM
  • parathyroid gland: 13 nTPM

Single-cell type

  • sertoli cells: 485 nCPM
  • thyrotrophs: 292 nCPM
  • myonuclei: 275 nCPM
  • neutrophil progenitors: 250 nCPM
  • somatotrophs: 230 nCPM
  • lactotrophs: 227 nCPM

Immune cell

  • basophil: 24 nTPM
  • NK-cell: 16 nTPM
  • eosinophil: 14 nTPM
  • MAIT T-cell: 14 nTPM
  • non-classical monocyte: 13 nTPM
  • gdT-cell: 12 nTPM

Brain region

  • cerebellum: 52 nTPM
  • cerebral cortex: 52 nTPM
  • hypothalamus: 48 nTPM
  • midbrain: 43 nTPM
  • basal ganglia: 42 nTPM
  • hippocampal formation: 42 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.14
gnomAD pLI
1
gnomAD missense Z
3.49
DepMap mean gene effect
-0.04
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 11% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of CNOT4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads CNOT4 as an antibody target. Whether an autoantibody or antibody against CNOT4 could matter depends on whether native CNOT4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

CNOT4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label CNOT4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/CNOT4. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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