Seroatlas · Human Serome Atlas

CNOT3

CCR4-NOT transcription complex subunit 3

Also known as: CNOT3_HUMAN, KIAA0691, LENG2, NOT3, NOT3H

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
O75175
Gene
CNOT3
Ensembl
ENSG00000088038
Chromosome
19
Canonical length
753 aa
Protein class
Cancer-related genes, Disease related genes, Human disease related genes, Predicted intracellular proteins
Subcellular location
Cytosol

OverviewNCBI Gene

Involved in regulation of stem cell population maintenance. Part of CCR4-NOT complex. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

753 residues, UniProt reviewed canonical sequence.

>O75175|CNOT3
     1  MADKRKLQGE IDRCLKKVSE GVEQFEDIWQ KLHNAANANQ KEKYEADLKK EIKKLQRLRD
    61  QIKTWVASNE IKDKRQLIDN RKLIETQMER FKVVERETKT KAYSKEGLGL AQKVDPAQKE
   121  KEEVGQWLTN TIDTLNMQVD QFESEVESLS VQTRKKKGDK DKQDRIEGLK RHIEKHRYHV
   181  RMLETILRML DNDSILVDAI RKIKDDVEYY VDSSQDPDFE ENEFLYDDLD LEDIPQALVA
   241  TSPPSHSHME DEIFNQSSST PTSTTSSSPI PPSPANCTTE NSEDDKKRGR STDSEVSQSP
   301  AKNGSKPVHS NQHPQSPAVP PTYPSGPPPA ASALSTTPGN NGVPAPAAPP SALGPKASPA
   361  PSHNSGTPAP YAQAVAPPAP SGPSTTQPRP PSVQPSGGGG GGSGGGGSSS SSNSSAGGGA
   421  GKQNGATSYS SVVADSPAEV ALSSSGGNNA SSQALGPPSG PHNPPPSTSK EPSAAAPTGA
   481  GGVAPGSGNN SGGPSLLVPL PVNPPSSPTP SFSDAKAAGA LLNGPPQFST APEIKAPEPL
   541  SSLKSMAERA AISSGIEDPV PTLHLTERDI ILSSTSAPPA SAQPPLQLSE VNIPLSLGVC
   601  PLGPVPLTKE QLYQQAMEEA AWHHMPHPSD SERIRQYLPR NPCPTPPYHH QMPPPHSDTV
   661  EFYQRLSTET LFFIFYYLEG TKAQYLAAKA LKKQSWRFHT KYMMWFQRHE EPKTITDEFE
   721  QGTYIYFDYE KWGQRKKEGF TFEYRYLEDR DLQ

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CNOT3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.54
Highest tissue expression
45 nTPM

Expression across tissuesHPA

Tissue

  • cerebellum: 45 nTPM
  • pituitary gland: 40 nTPM
  • testis: 36 nTPM
  • thyroid gland: 36 nTPM
  • ovary: 35 nTPM
  • spleen: 33 nTPM

Single-cell type

  • tuft cells: 35 nCPM
  • early primary spermatocytes: 16 nCPM
  • astrocytes: 15 nCPM
  • oocytes: 14 nCPM
  • ependymal cells: 14 nCPM
  • oligodendrocytes: 13 nCPM

Immune cell

  • neutrophil: 4.2 nTPM
  • plasmacytoid DC: 3.4 nTPM
  • eosinophil: 3.1 nTPM
  • total PBMC: 2 nTPM
  • classical monocyte: 1.6 nTPM
  • gdT-cell: 1.6 nTPM

Brain region

  • cerebral cortex: 38 nTPM
  • cerebellum: 32 nTPM
  • hypothalamus: 32 nTPM
  • medulla oblongata: 29 nTPM
  • amygdala: 29 nTPM
  • white matter: 28 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about CNOT3.

Disease | AllUniProt

Conditions CNOT3 is implicated in, by any mechanism.

  • Intellectual developmental disorder with speech delay, autism and dysmorphic facies (IDDSADF) MIM:618672

Disease | GeneticClinVar

71 pathogenic / likely-pathogenic of 455 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.08
gnomAD pLI
1
gnomAD missense Z
3.76
DepMap mean gene effect
-1.52
DepMap dependency class
pan

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 8% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of CNOT3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads CNOT3 as an antibody target. Whether an autoantibody or antibody against CNOT3 could matter depends on whether native CNOT3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

CNOT3 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label CNOT3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/CNOT3. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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