Seroatlas · Human Serome Atlas

UBE2D4

Ubiquitin-conjugating enzyme E2 D4

Also known as: HBUCE1, UB2D4_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9Y2X8
Gene: UBE2D4
Ensembl: ENSG00000078967
Chromosome: 7
Canonical length: 147 aa
Protein class: Enzymes, Metabolic proteins, Predicted intracellular proteins
Subcellular location: Plasma membrane,Cytosol

OverviewNCBI Gene

Enables ubiquitin conjugating enzyme activity. Involved in protein polyubiquitination. Acts upstream of or within protein ubiquitination. Predicted to be active in nucleus. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

147 residues, UniProt reviewed canonical sequence.

>Q9Y2X8|UBE2D4
     1  MALKRIQKEL TDLQRDPPAQ CSAGPVGDDL FHWQATIMGP NDSPYQGGVF FLTIHFPTDY
    61  PFKPPKVAFT TKIYHPNINS NGSICLDILR SQWSPALTVS KVLLSICSLL CDPNPDDPLV
   121  PEIAHTYKAD REKYNRLARE WTQKYAM

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against UBE2D4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.28
Highest tissue expression: 30 nTPM

Expression across tissuesHPA

Tissue

skeletal muscle: 30 nTPM
tongue: 26 nTPM
hippocampal formation: 11 nTPM
amygdala: 11 nTPM
cerebral cortex: 11 nTPM
heart muscle: 11 nTPM

Single-cell type

cardiomyocytes: 100 nCPM
epicardial cells: 48 nCPM
myonuclei: 42 nCPM
esophageal suprabasal cells: 37 nCPM
breast lactating cells: 36 nCPM
hofbauer cells: 35 nCPM

Immune cell

non-classical monocyte: 29 nTPM
intermediate monocyte: 24 nTPM
eosinophil: 18 nTPM
naive CD4 T-cell: 16 nTPM
myeloid DC: 15 nTPM
classical monocyte: 15 nTPM

Brain region

cerebellum: 20 nTPM
white matter: 16 nTPM
hippocampal formation: 16 nTPM
basal ganglia: 16 nTPM
medulla oblongata: 16 nTPM
spinal cord: 16 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.47
gnomAD pLI: 0
gnomAD missense Z: 0.92
DepMap mean gene effect: -0.2
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 10% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

nucleus

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of UBE2D4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads UBE2D4 as an antibody target. Whether an autoantibody or antibody against UBE2D4 could matter depends on whether native UBE2D4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

UBE2D4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label UBE2D4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Loading the interactive Seroatlas protein explorer...