Seroatlas · Human Serome Atlas

RNF5

E3 ubiquitin-protein ligase RNF5

Also known as: G16, NG2, RING5, RMA1, RNF5_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q99942
Gene: RNF5
Ensembl: ENSG00000204308
Chromosome: 6
Canonical length: 180 aa
Protein class: Enzymes, Metabolic proteins, Predicted membrane proteins
Subcellular location: Endoplasmic reticulum

OverviewNCBI Gene

The protein encoded by this gene contains a RING finger, which is a motif known to be involved in protein-protein interactions. This protein is a membrane-bound ubiquitin ligase. It can regulate cell motility by targeting paxillin ubiquitination and altering the distribution and localization of paxillin in cytoplasm and cell focal adhesions. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

180 residues, UniProt reviewed canonical sequence.

>Q99942|RNF5
     1  MAAAEEEDGG PEGPNRERGG AGATFECNIC LETAREAVVS VCGHLYCWPC LHQWLETRPE
    61  RQECPVCKAG ISREKVVPLY GRGSQKPQDP RLKTPPRPQG QRPAPESRGG FQPFGDTGGF
   121  HFSFGVGAFP FGFFTTVFNA HEPFRRGTGV DLGQGHPASS WQDSLFLFLA IFFFFWLLSI

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RNF5 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Cell surface
Secreted: No
Transmembrane segments: 2
Mean surface accessibility (rSASA): 0.56
Highest tissue expression: 118 nTPM

Expression across tissuesHPA

Tissue

liver: 118 nTPM
kidney: 108 nTPM
skeletal muscle: 84 nTPM
heart muscle: 82 nTPM
spinal cord: 79 nTPM
adrenal gland: 77 nTPM

Single-cell type

oligodendrocytes: 14 nCPM
renal collecting duct principal cells: 12 nCPM
loop of henle epithelial cells: 8.9 nCPM
papillary tip epithelial cells: 8.8 nCPM
bergmann glia: 8.4 nCPM
ependymal cells: 8 nCPM

Immune cell

plasmacytoid DC: 265 nTPM
NK-cell: 80 nTPM
eosinophil: 76 nTPM
total PBMC: 75 nTPM
intermediate monocyte: 66 nTPM
gdT-cell: 65 nTPM

Brain region

white matter: 45 nTPM
medulla oblongata: 42 nTPM
cerebellum: 41 nTPM
basal ganglia: 38 nTPM
spinal cord: 38 nTPM
thalamus: 36 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.33
gnomAD pLI: 0
gnomAD missense Z: 0.79
DepMap mean gene effect: -0.04
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 8% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of RNF5 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads RNF5 as an antibody target. Whether an autoantibody or antibody against RNF5 could matter depends on whether native RNF5 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

RNF5 is annotated at the cell surface, where native RNF5 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label RNF5 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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