Seroatlas · Human Serome Atlas

RNF25

E3 ubiquitin-protein ligase RNF25

Also known as: AO7, FLJ13906, RNF25_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q96BH1
Gene
RNF25
Ensembl
ENSG00000163481
Chromosome
2
Canonical length
459 aa
Protein class
Enzymes, Metabolic proteins, Predicted intracellular proteins
Subcellular location
Nucleoplasm,Actin filaments,Cytosol

OverviewNCBI Gene

The protein encoded by this gene contains a RING finger motif. The mouse counterpart of this protein has been shown to interact with Rela, the p65 subunit of NF-kappaB (NFKB), and modulate NFKB-mediated transcription activity. The mouse protein also binds ubiquitin-conjugating enzymes (E2s) and is a substrate for E2-dependent ubiquitination. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

459 residues, UniProt reviewed canonical sequence.

>Q96BH1|RNF25
     1  MAASASAAAG EEDWVLPSEV EVLESIYLDE LQVIKGNGRT SPWEIYITLH PATAEDQDSQ
    61  YVCFTLVLQV PAEYPHEVPQ ISIRNPRGLS DEQIHTILQV LGHVAKAGLG TAMLYELIEK
   121  GKEILTDNNI PHGQCVICLY GFQEKEAFTK TPCYHYFHCH CLARYIQHME QELKAQGQEQ
   181  EQERQHATTK QKAVGVQCPV CREPLVYDLA SLKAAPEPQQ PMELYQPSAE SLRQQEERKR
   241  LYQRQQERGG IIDLEAERNR YFISLQQPPA PAEPESAVDV SKGSQPPSTL AAELSTSPAV
   301  QSTLPPPLPV ATQHICEKIP GTRSNQQRLG ETQKAMLDPP KPSRGPWRQP ERRHPKGGEC
   361  HAPKGTRDTQ ELPPPEGPLK EPMDLKPEPH SQGVEGPPQE KGPGSWQGPP PRRTRDCVRW
   421  ERSKGRTPGS SYPRLPRGQG AYRPGTRRES LGLESKDGS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RNF25 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.52
Highest tissue expression
24 nTPM

Expression across tissuesHPA

Tissue

  • blood vessel: 24 nTPM
  • testis: 23 nTPM
  • skeletal muscle: 22 nTPM
  • hypothalamus: 22 nTPM
  • pituitary gland: 21 nTPM
  • choroid plexus: 21 nTPM

Single-cell type

  • late primary spermatocytes: 29 nCPM
  • decidual stromal cells: 28 nCPM
  • oocytes: 28 nCPM
  • cardiomyocytes: 19 nCPM
  • hofbauer cells: 18 nCPM
  • rod photoreceptor cells: 16 nCPM

Immune cell

  • basophil: 58 nTPM
  • eosinophil: 33 nTPM
  • NK-cell: 31 nTPM
  • naive B-cell: 31 nTPM
  • naive CD4 T-cell: 29 nTPM
  • memory CD8 T-cell: 27 nTPM

Brain region

  • hypothalamus: 15 nTPM
  • pons: 13 nTPM
  • white matter: 13 nTPM
  • cerebral cortex: 12 nTPM
  • spinal cord: 12 nTPM
  • basal ganglia: 12 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.52
gnomAD pLI
0.02
gnomAD missense Z
0.63
DepMap mean gene effect
-0.19
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of RNF25 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads RNF25 as an antibody target. Whether an autoantibody or antibody against RNF25 could matter depends on whether native RNF25 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

RNF25 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label RNF25 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/RNF25. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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