Seroatlas · Human Serome Atlas

RFWD3

E3 ubiquitin-protein ligase RFWD3

Also known as: FANCW, FLJ10520, RFWD3_HUMAN, RNF201

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q6PCD5
Gene: RFWD3
Ensembl: ENSG00000168411
Chromosome: 16
Canonical length: 774 aa
Protein class: Disease related genes, Enzymes, Human disease related genes, Metabolic proteins, Potential drug targets, Predicted intracellular proteins
Subcellular location: Nucleoplasm,Cytosol

OverviewNCBI Gene

Enables MDM2/MDM4 family protein binding activity; p53 binding activity; and ubiquitin protein ligase activity. Involved in several processes, including DNA metabolic process; regulation of cell cycle phase transition; and response to ionizing radiation. Located in nucleoplasm and site of double-strand break. Implicated in Fanconi anemia complementation group W. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

774 residues, UniProt reviewed canonical sequence.

>Q6PCD5|RFWD3
     1  MAHEAMEYDV QVQLNHAEQQ PAPAGMASSQ GGPALLQPVP ADVVSSQGVP SILQPAPAEV
    61  ISSQATPPLL QPAPQLSVDL TEVEVLGEDT VENINPRTSE QHRQGSDGNH TIPASSLHSM
   121  TNFISGLQRL HGMLEFLRPS SSNHSVGPMR TRRRVSASRR ARAGGSQRTD SARLRAPLDA
   181  YFQVSRTQPD LPATTYDSET RNPVSEELQV SSSSDSDSDS SAEYGGVVDQ AEESGAVILE
   241  EQLAGVSAEQ EVTCIDGGKT LPKQPSPQKS EPLLPSASMD EEEGDTCTIC LEQWTNAGDH
   301  RLSALRCGHL FGYRCISTWL KGQVRKCPQC NKKARHSDIV VLYARTLRAL DTSEQERMKS
   361  SLLKEQMLRK QAELESAQCR LQLQVLTDKC TRLQRRVQDL QKLTSHQSQN LQQPRGSQAW
   421  VLSCSPSSQG QHKHKYHFQK TFTVSQAGNC RIMAYCDALS CLVISQPSPQ ASFLPGFGVK
   481  MLSTANMKSS QYIPMHGKQI RGLAFSSYLR GLLLSASLDN TIKLTSLETN TVVQTYNAGR
   541  PVWSCCWCLD EANYIYAGLA NGSILVYDVR NTSSHVQELV AQKARCPLVS LSYMPRAASA
   601  AFPYGGVLAG TLEDASFWEQ KMDFSHWPHV LPLEPGGCID FQTENSSRHC LVTYRPDKNH
   661  TTIRSVLMEM SYRLDDTGNP ICSCQPVHTF FGGPTCKLLT KNAIFQSPEN DGNILVCTGD
   721  EAANSALLWD AASGSLLQDL QTDQPVLDIC PFEVNRNSYL ATLTEKMVHI YKWE

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RFWD3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.45
Highest tissue expression: 45 nTPM

Expression across tissuesHPA

Tissue

bone marrow: 45 nTPM
thymus: 25 nTPM
testis: 24 nTPM
tonsil: 16 nTPM
lymph node: 14 nTPM
skin: 12 nTPM

Single-cell type

neutrophil progenitors: 120 nCPM
early primary spermatocytes: 117 nCPM
erythrocyte progenitors: 98 nCPM
monocyte progenitors: 91 nCPM
megakaryocyte progenitors: 63 nCPM
undifferentiated spermatogonia: 48 nCPM

Immune cell

basophil: 12 nTPM
non-classical monocyte: 7.7 nTPM
T-reg: 7.3 nTPM
naive CD4 T-cell: 6.7 nTPM
eosinophil: 6.5 nTPM
NK-cell: 6.2 nTPM

Brain region

white matter: 11 nTPM
choroid plexus: 11 nTPM
cerebellum: 9.7 nTPM
thalamus: 9.4 nTPM
basal ganglia: 8.8 nTPM
medulla oblongata: 8.7 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about RFWD3.

Disease | AllUniProt

Conditions RFWD3 is implicated in, by any mechanism.

Fanconi anemia, complementation group W (FANCW) MIM:617784

Disease | GeneticClinVar

3 pathogenic / likely-pathogenic of 722 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

Fanconi anemia, complementation group W
Fanconi anemia

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.78
gnomAD pLI: 0
gnomAD missense Z: -0.89
DepMap mean gene effect: -0.36
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

WD40 repeat
Zinc finger, RING-type
Zinc finger, RING/FYVE/PHD-type
WD40/YVTN repeat-like-containing domain superfamily
WD40-repeat-containing domain superfamily
Ring finger domain
E3 ubiquitin-protein ligase RFWD3
E3 ubiquitin-protein ligase RFWD3-like, WD40 domain
E3 ubiquitin-protein ligase RFWD3 WD40 domain

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of RFWD3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads RFWD3 as an antibody target. Whether an autoantibody or antibody against RFWD3 could matter depends on whether native RFWD3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

RFWD3 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label RFWD3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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