Seroatlas · Human Serome Atlas

ZNRF1

E3 ubiquitin-protein ligase ZNRF1

Also known as: DKFZp434E229, FLJ14846, nin283, ZNRF1_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q8ND25
Gene: ZNRF1
Ensembl: ENSG00000186187
Chromosome: 16
Canonical length: 227 aa
Protein class: Enzymes, Metabolic proteins, Predicted intracellular proteins
Subcellular location: Vesicles,Primary cilium tip,Primary cilium transition zone

OverviewNCBI Gene

This gene encodes an E3 ubiquitin-protein ligase that plays a role in neural-cell differentiation. Overexpression of this gene causes neurite-like elongation. The encoded protein contains both a zinc finger and a RING finger motif and is localized in the endosome/lysosome compartment, indicating that it may be involved in ubiquitin-mediated protein modification, and in synaptic vessicle membranes in neurons. [provided by RefSeq, Feb 2012]

Canonical amino-acid sequenceUniProt

227 residues, UniProt reviewed canonical sequence.

>Q8ND25|ZNRF1
     1  MGGKQSTAAR SRGPFPGVST DDSAVPPPGG APHFGHYRTG GGAMGLRSRS VSSVAGMGMD
    61  PSTAGGVPFG LYTPASRGTG DSERAPGGGG SASDSTYAHG NGYQETGGGH HRDGMLYLGS
   121  RASLADALPL HIAPRWFSSH SGFKCPICSK SVASDEMEMH FIMCLSKPRL SYNDDVLTKD
   181  AGECVICLEE LLQGDTIARL PCLCIYHKSC IDSWFEVNRS CPEHPAD

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ZNRF1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.6
Highest tissue expression: 76 nTPM

Expression across tissuesHPA

Tissue

skeletal muscle: 76 nTPM
esophagus: 64 nTPM
thymus: 50 nTPM
testis: 36 nTPM
cerebral cortex: 29 nTPM
colon: 29 nTPM

Single-cell type

esophageal apical cells: 485 nCPM
hematopoietic stem cells: 407 nCPM
megakaryocyte-erythroid progenitors: 270 nCPM
mast cells: 240 nCPM
thymocytes: 226 nCPM
myonuclei: 218 nCPM

Immune cell

T-reg: 2.5 nTPM
basophil: 2.4 nTPM
memory CD4 T-cell: 0.6 nTPM
classical monocyte: 0.5 nTPM
intermediate monocyte: 0.5 nTPM
MAIT T-cell: 0.5 nTPM

Brain region

hypothalamus: 72 nTPM
midbrain: 56 nTPM
cerebral cortex: 56 nTPM
basal ganglia: 53 nTPM
amygdala: 50 nTPM
medulla oblongata: 49 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.39
gnomAD pLI: 0.91
gnomAD missense Z: 0.92
DepMap mean gene effect: 0.1
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of ZNRF1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ZNRF1 as an antibody target. Whether an autoantibody or antibody against ZNRF1 could matter depends on whether native ZNRF1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ZNRF1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ZNRF1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Loading the interactive Seroatlas protein explorer...