Seroatlas · Human Serome Atlas

RNF185

E3 ubiquitin-protein ligase RNF185

Also known as: FLJ38628, RN185_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q96GF1
Gene: RNF185
Ensembl: ENSG00000138942
Chromosome: 22
Canonical length: 192 aa
Protein class: Enzymes, Metabolic proteins, Predicted membrane proteins

OverviewNCBI Gene

Enables ubiquitin binding activity; ubiquitin protein ligase activity; and ubiquitin-like protein conjugating enzyme binding activity. Involved in several processes, including positive regulation of ERAD pathway; protein ubiquitination; and proteolysis involved in protein catabolic process. Located in endoplasmic reticulum. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

192 residues, UniProt reviewed canonical sequence.

>Q96GF1|RNF185
     1  MASKGPSASA SPENSSAGGP SGSSNGAGES GGQDSTFECN ICLDTAKDAV ISLCGHLFCW
    61  PCLHQWLETR PNRQVCPVCK AGISRDKVIP LYGRGSTGQQ DPREKTPPRP QGQRPEPENR
   121  GGFQGFGFGD GGFQMSFGIG AFPFGIFATA FNINDGRPPP AVPGTPQYVD EQFLSRLFLF
   181  VALVIMFWLL IA

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RNF185 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Other membrane
Secreted: No
Transmembrane segments: 2
Mean surface accessibility (rSASA): 0.57
Highest tissue expression: 48 nTPM

Expression across tissuesHPA

Tissue

thyroid gland: 48 nTPM
parathyroid gland: 46 nTPM
liver: 45 nTPM
prostate: 36 nTPM
kidney: 34 nTPM
blood vessel: 34 nTPM

Single-cell type

neutrophils: 153 nCPM
prostatic glandular cells: 79 nCPM
podocytes: 69 nCPM
choroid plexus epithelial cells: 67 nCPM
syncytiotrophoblasts: 65 nCPM
myonuclei: 61 nCPM

Immune cell

neutrophil: 22 nTPM
basophil: 17 nTPM
NK-cell: 17 nTPM
intermediate monocyte: 16 nTPM
classical monocyte: 16 nTPM
total PBMC: 15 nTPM

Brain region

thalamus: 25 nTPM
white matter: 24 nTPM
choroid plexus: 23 nTPM
hippocampal formation: 23 nTPM
cerebral cortex: 23 nTPM
midbrain: 23 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.72
gnomAD pLI: 0.18
gnomAD missense Z: 1.05
DepMap mean gene effect: -0.06
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of RNF185 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads RNF185 as an antibody target. Whether an autoantibody or antibody against RNF185 could matter depends on whether native RNF185 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

RNF185 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label RNF185 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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