Seroatlas · Human Serome Atlas

MID2

Probable E3 ubiquitin-protein ligase MID2

Also known as: FXY2, MRX101, RNF60, TRIM1, TRIM1_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q9UJV3
Gene
MID2
Ensembl
ENSG00000080561
Chromosome
X
Canonical length
735 aa
Protein class
Disease related genes, Enzymes, Human disease related genes, Potential drug targets, Predicted intracellular proteins
Quaternary structure
Homodimer

OverviewNCBI Gene

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to microtubular structures in the cytoplasm. Alternate splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Feb 2009]

Canonical amino-acid sequenceUniProt

735 residues, UniProt reviewed canonical sequence.

>Q9UJV3|MID2
     1  MGESPASVVL NASGGLFSLK METLESELTC PICLELFEDP LLLPCAHSLC FSCAHRILVS
    61  SCSSGESIEP ITAFQCPTCR YVISLNHRGL DGLKRNVTLQ NIIDRFQKAS VSGPNSPSES
   121  RRERTYRPTT AMSSERIACQ FCEQDPPRDA VKTCITCEVS YCDRCLRATH PNKKPFTSHR
   181  LVEPVPDTHL RGITCLDHEN EKVNMYCVSD DQLICALCKL VGRHRDHQVA SLNDRFEKLK
   241  QTLEMNLTNL VKRNSELENQ MAKLIQICQQ VEVNTAMHEA KLMEECDELV EIIQQRKQMI
   301  AVKIKETKVM KLRKLAQQVA NCRQCLERST VLINQAEHIL KENDQARFLQ SAKNIAERVA
   361  MATASSQVLI PDINFNDAFE NFALDFSREK KLLEGLDYLT APNPPSIREE LCTASHDTIT
   421  VHWISDDEFS ISSYELQYTI FTGQANFISK SWCSWGLWPE IRKCKEAVSC SRLAGAPRGL
   481  YNSVDSWMIV PNIKQNHYTV HGLQSGTRYI FIVKAINQAG SRNSEPTRLK TNSQPFKLDP
   541  KMTHKKLKIS NDGLQMEKDE SSLKKSHTPE RFSGTGCYGA AGNIFIDSGC HYWEVVMGSS
   601  TWYAIGIAYK SAPKNEWIGK NASSWVFSRC NSNFVVRHNN KEMLVDVPPH LKRLGVLLDY
   661  DNNMLSFYDP ANSLHLHTFD VTFILPVCPT FTIWNKSLMI LSGLPAPDFI DYPERQECNC
   721  RPQESPYVSG MKTCH

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MID2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.39
Highest tissue expression
17 nTPM

Expression across tissuesHPA

Tissue

  • tongue: 17 nTPM
  • smooth muscle: 15 nTPM
  • retina: 14 nTPM
  • esophagus: 13 nTPM
  • thyroid gland: 12 nTPM
  • parathyroid gland: 12 nTPM

Single-cell type

  • urothelial cells: 108 nCPM
  • prostatic glandular cells: 74 nCPM
  • lymphatic endothelial cells: 68 nCPM
  • esophageal apical cells: 64 nCPM
  • pituicytes/fscs: 55 nCPM
  • rod photoreceptor cells: 49 nCPM

Immune cell

  • naive CD4 T-cell: 1.6 nTPM
  • memory CD8 T-cell: 1.4 nTPM
  • MAIT T-cell: 1.3 nTPM
  • naive CD8 T-cell: 1.1 nTPM
  • memory CD4 T-cell: 0.8 nTPM
  • gdT-cell: 0.6 nTPM

Brain region

  • hypothalamus: 35 nTPM
  • pons: 30 nTPM
  • thalamus: 26 nTPM
  • medulla oblongata: 25 nTPM
  • midbrain: 25 nTPM
  • spinal cord: 23 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about MID2.

Disease | AllUniProt

Conditions MID2 is implicated in, by any mechanism.

  • Intellectual developmental disorder, X-linked 101 (XLID101) MIM:300928

Disease | GeneticClinVar

3 pathogenic / likely-pathogenic of 213 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

  • Intellectual disability, X-linked 101

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.5
gnomAD pLI
0.18
gnomAD missense Z
1.58
DepMap mean gene effect
-0.09
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of MID2 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads MID2 as an antibody target. Whether an autoantibody or antibody against MID2 could matter depends on whether native MID2 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

MID2 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label MID2 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/MID2. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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