Seroatlas · Human Serome Atlas

RNF166

E3 ubiquitin-protein ligase RNF166

Also known as: MGC14381, MGC2647, RN166_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q96A37
Gene: RNF166
Ensembl: ENSG00000158717
Chromosome: 16
Canonical length: 237 aa
Protein class: Enzymes, Predicted intracellular proteins
Subcellular location: Nucleoplasm,Plasma membrane,Cytosol

OverviewNCBI Gene

Predicted to enable ubiquitin protein ligase activity. Predicted to be involved in protein polyubiquitination and ubiquitin-dependent protein catabolic process. Predicted to be located in cytoplasm. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

237 residues, UniProt reviewed canonical sequence.

>Q96A37|RNF166
     1  MAMFRSLVAS AQQRQPPAGP AGGDSGLEAQ YTCPICLEVY HRPVAIGSCG HTFCGECLQP
    61  CLQVPSPLCP LCRLPFDPKK VDKATHVEKQ LSSYKAPCRG CNKKVTLAKM RVHISSCLKV
   121  QEQMANCPKF VPVVPTSQPI PSNIPNRSTF ACPYCGARNL DQQELVKHCV ESHRSDPNRV
   181  VCPICSAMPW GDPSYKSANF LQHLLHRHKF SYDTFVDYSI DEEAAFQAAL ALSLSEN

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RNF166 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.42
Highest tissue expression: 99 nTPM

Expression across tissuesHPA

Tissue

spleen: 99 nTPM
bone marrow: 86 nTPM
lung: 47 nTPM
lymph node: 38 nTPM
small intestine: 35 nTPM
cerebellum: 28 nTPM

Single-cell type

neutrophils: 153 nCPM
nk-cells: 82 nCPM
neutrophil progenitors: 71 nCPM
monocytes: 68 nCPM
microglia: 65 nCPM
t-cells: 60 nCPM

Immune cell

eosinophil: 62 nTPM
neutrophil: 41 nTPM
gdT-cell: 32 nTPM
MAIT T-cell: 28 nTPM
memory CD8 T-cell: 28 nTPM
classical monocyte: 26 nTPM

Brain region

cerebral cortex: 39 nTPM
thalamus: 33 nTPM
white matter: 30 nTPM
medulla oblongata: 30 nTPM
pons: 26 nTPM
spinal cord: 25 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.61
gnomAD pLI: 0.49
gnomAD missense Z: 0.73
DepMap mean gene effect: -0.05
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

cytoplasm

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of RNF166 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads RNF166 as an antibody target. Whether an autoantibody or antibody against RNF166 could matter depends on whether native RNF166 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

RNF166 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label RNF166 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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