Seroatlas · Human Serome Atlas

RPA2

Replication protein A 32 kDa subunit

Also known as: RFA2_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P15927
Gene
RPA2
Ensembl
ENSG00000117748
Chromosome
1
Canonical length
270 aa
Protein class
Cancer-related genes, Plasma proteins, Predicted intracellular proteins
Subcellular location
Nucleoplasm,Nuclear bodies

OverviewNCBI Gene

This gene encodes a subunit of the heterotrimeric Replication Protein A (RPA) complex, which binds to single-stranded DNA (ssDNA), forming a nucleoprotein complex that plays an important role in DNA metabolism, being involved in DNA replication, repair, recombination, telomere maintenance, and co-ordinating the cellular response to DNA damage through activation of the ataxia telangiectasia and Rad3-related protein (ATR) kinase. The RPA complex protects single-stranded DNA from nucleases, prevents formation of secondary structures that would interfere with repair, and co-ordinates the recruitment and departure of different genome maintenance factors. The heterotrimeric complex has two different modes of ssDNA binding, a low-affinity and high-affinity mode, determined by which oligonucleotide/oligosaccharide-binding (OB) domains of the complex are utilized, and differing in the length of DNA bound. This subunit contains a single OB domain that participates in high-affinity DNA binding and also contains a winged helix domain at its carboxy terminus, which interacts with many genome maintenance protein. Post-translational modifications of the RPA complex also plays a role in co-ordinating different damage response pathways. [provided by RefSeq, Sep 2017]

Canonical amino-acid sequenceUniProt

270 residues, UniProt reviewed canonical sequence.

>P15927|RPA2
     1  MWNSGFESYG SSSYGGAGGY TQSPGGFGSP APSQAEKKSR ARAQHIVPCT ISQLLSATLV
    61  DEVFRIGNVE ISQVTIVGII RHAEKAPTNI VYKIDDMTAA PMDVRQWVDT DDTSSENTVV
   121  PPETYVKVAG HLRSFQNKKS LVAFKIMPLE DMNEFTTHIL EVINAHMVLS KANSQPSAGR
   181  APISNPGMSE AGNFGGNSFM PANGLTVAQN QVLNLIKACP RPEGLNFQDL KNQLKHMSVS
   241  SIKQAVDFLS NEGHIYSTVD DDHFKSTDAE

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RPA2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.42
Highest tissue expression
76 nTPM

Expression across tissuesHPA

Tissue

  • skeletal muscle: 76 nTPM
  • lymph node: 70 nTPM
  • tonsil: 63 nTPM
  • testis: 60 nTPM
  • tongue: 59 nTPM
  • ovary: 53 nTPM

Single-cell type

  • oocytes: 267 nCPM
  • early primary spermatocytes: 225 nCPM
  • differentiating spermatogonia: 183 nCPM
  • neutrophils: 118 nCPM
  • cytotrophoblasts: 117 nCPM
  • late primary spermatocytes: 106 nCPM

Immune cell

  • total PBMC: 436 nTPM
  • basophil: 324 nTPM
  • MAIT T-cell: 305 nTPM
  • NK-cell: 295 nTPM
  • eosinophil: 291 nTPM
  • naive CD4 T-cell: 288 nTPM

Brain region

  • choroid plexus: 36 nTPM
  • spinal cord: 34 nTPM
  • white matter: 34 nTPM
  • hypothalamus: 34 nTPM
  • basal ganglia: 30 nTPM
  • medulla oblongata: 29 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about RPA2.

Disease | ImmuneIEDB

Conditions an epitope on RPA2 was assayed in.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.52
gnomAD pLI
0.5
gnomAD missense Z
0.77
DepMap mean gene effect
-1.82
DepMap dependency class
pan

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of RPA2 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads RPA2 as an antibody target. Whether an autoantibody or antibody against RPA2 could matter depends on whether native RPA2 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

RPA2 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label RPA2 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/RPA2. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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