FBH1

Protein identityUniProt · HPA

UniProt accession

Q8NFZ0

Gene

FBH1

Ensembl

ENSG00000134452

Chromosome

10

Canonical length

1043 aa

Protein class

Disease related genes, Enzymes, Plasma proteins, Potential drug targets, Predicted membrane proteins

OverviewNCBI Gene

This gene encodes a member of the F-box protein family, members of which are characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into three classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbx class. It contains an F-box motif and seven conserved helicase motifs, and has both DNA-dependent ATPase and DNA unwinding activities. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

1043 residues, UniProt reviewed canonical sequence.

>Q8NFZ0|FBH1
     1  MRRFKRKHLT AIDCQHLARS HLAVTQPFGQ RWTNRDPNHG LYPKPRTKRG SRGQGSQRCI
    61  PEFFLAGKQP CTNDMAKSNS VGQDSCQDSE GDMIFPAESS CALPQEGSAG PGSPGSAPPS
   121  RKRSWSSEEE SNQATGTSRW DGVSKKAPRH HLSVPCTRPR EARQEAEDST SRLSAESGET
   181  DQDAGDVGPD PIPDSYYGLL GTLPCQEALS HICSLPSEVL RHVFAFLPVE DLYWNLSLVC
   241  HLWREIISDP LFIPWKKLYH RYLMNEEQAV SKVDGILSNC GIEKESDLCV LNLIRYTATT
   301  KCSPSVDPER VLWSLRDHPL LPEAEACVRQ HLPDLYAAAG GVNIWALVAA VVLLSSSVND
   361  IQRLLFCLRR PSSTVTMPDV TETLYCIAVL LYAMREKGIN ISNRIHYNIF YCLYLQENSC
   421  TQATKVKEEP SVWPGKKTIQ LTHEQQLILN HKMEPLQVVK IMAFAGTGKT STLVKYAEKW
   481  SQSRFLYVTF NKSIAKQAER VFPSNVICKT FHSMAYGHIG RKYQSKKKLN LFKLTPFMVN
   541  SVLAEGKGGF IRAKLVCKTL ENFFASADEE LTIDHVPIWC KNSQGQRVMV EQSEKLNGVL
   601  EASRLWDNMR KLGECTEEAH QMTHDGYLKL WQLSKPSLAS FDAIFVDEAQ DCTPAIMNIV
   661  LSQPCGKIFV GDPHQQIYTF RGAVNALFTV PHTHVFYLTQ SFRFGVEIAY VGATILDVCK
   721  RVRKKTLVGG NHQSGIRGDA KGQVALLSRT NANVFDEAVR VTEGEFPSRI HLIGGIKSFG
   781  LDRIIDIWIL LQPEEERRKQ NLVIKDKFIR RWVHKEGFSG FKRYVTAAED KELEAKIAVV
   841  EKYNIRIPEL VQRIEKCHIE DLDFAEYILG TVHKAKGLEF DTVHVLDDFV KVPCARHNLP
   901  QLPHFRVESF SEDEWNLLYV AVTRAKKRLI MTKSLENILT LAGEYFLQAE LTSNVLKTGV
   961  VRCCVGQCNN AIPVDTVLTM KKLPITYSNR KENKGGYLCH SCAEQRIGPL AFLTASPEQV
  1021  RAMERTVENI VLPRHEALLF LVF

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against FBH1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.34

Highest tissue expression

42 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.48

gnomAD pLI

0

DepMap mean gene effect

0

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 9% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• DNA catabolic process
• DNA damage response
• DNA repair
• double-strand break repair via homologous recombination
• negative regulation of double-strand break repair via homologous recombination
• positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage
• positive regulation of protein phosphorylation
• protein ubiquitination
• replication fork processing
• response to intra-S DNA damage checkpoint signaling

Molecular functions

• 3'-5' DNA helicase activity
• ATP binding
• ATP hydrolysis activity
• DNA helicase activity
• DNA translocase activity
• double-stranded DNA binding
• single-stranded DNA binding

Cellular components

• chromatin
• nucleus
• SCF ubiquitin ligase complex

Protein domainsUniProt · Pfam · InterPro

• F-box domain
• UvrD-like helicase, ATP-binding domain
• P-loop containing nucleoside triphosphate hydrolase
• F-box-like domain superfamily
• UvrD/REP helicase N-terminal domain
• F-box-like
• UvrD-like helicase
• UvrD-like helicase, C-terminal
• UvrD-like helicase C-terminal domain

KeywordsUniProt

• ATP-binding
• Chromosome
• DNA damage
• DNA repair
• DNA-binding
• Helicase
• Hydrolase
• Isomerase
• Nucleotide-binding
• Nucleus
• Phosphoprotein
• Ubl conjugation
• Ubl conjugation pathway

InteractionsUniProt · HPA

Protein binding partners of FBH1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• RAD51
• RPA2