Seroatlas · Human Serome Atlas

CDC45

Cell division control protein 45 homolog

Also known as: CDC45_HUMAN, CDC45L, CDC45L2

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
O75419
Gene
CDC45
Ensembl
ENSG00000093009
Chromosome
22
Canonical length
566 aa
Protein class
Disease related genes, Human disease related genes, Plasma proteins, Predicted intracellular proteins
Subcellular location
Nucleoplasm,Centrosome,Cytosol

OverviewNCBI Gene

The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Canonical amino-acid sequenceUniProt

566 residues, UniProt reviewed canonical sequence.

>O75419|CDC45
     1  MFVSDFRKEF YEVVQSQRVL LFVASDVDAL CACKILQALF QCDHVQYTLV PVSGWQELET
    61  AFLEHKEQFH YFILINCGAN VDLLDILQPD EDTIFFVCDT HRPVNVVNVY NDTQIKLLIK
   121  QDDDLEVPAY EDIFRDEEED EEHSGNDSDG SEPSEKRTRL EEEIVEQTMR RRQRREWEAR
   181  RRDILFDYEQ YEYHGTSSAM VMFELAWMLS KDLNDMLWWA IVGLTDQWVQ DKITQMKYVT
   241  DVGVLQRHVS RHNHRNEDEE NTLSVDCTRI SFEYDLRLVL YQHWSLHDSL CNTSYTAARF
   301  KLWSVHGQKR LQEFLADMGL PLKQVKQKFQ AMDISLKENL REMIEESANK FGMKDMRVQT
   361  FSIHFGFKHK FLASDVVFAT MSLMESPEKD GSGTDHFIQA LDSLSRSNLD KLYHGLELAK
   421  KQLRATQQTI ASCLCTNLVI SQGPFLYCSL MEGTPDVMLF SRPASLSLLS KHLLKSFVCS
   481  TKNRRCKLLP LVMAAPLSME HGTVTVVGIP PETDSSDRKN FFGRAFEKAA ESTSSRMLHN
   541  HFDLSVIELK AEDRSKFLDA LISLLS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CDC45 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.24
Highest tissue expression
27 nTPM

Expression across tissuesHPA

Tissue

  • testis: 27 nTPM
  • bone marrow: 24 nTPM
  • thymus: 19 nTPM
  • tonsil: 11 nTPM
  • lymph node: 11 nTPM
  • appendix: 5.8 nTPM

Single-cell type

  • early spermatids: 132 nCPM
  • early primary spermatocytes: 116 nCPM
  • late primary spermatocytes: 109 nCPM
  • erythrocyte progenitors: 68 nCPM
  • differentiating spermatogonia: 57 nCPM
  • oocytes: 51 nCPM

Immune cell

  • T-reg: 0.9 nTPM
  • memory CD8 T-cell: 0.7 nTPM
  • total PBMC: 0.6 nTPM
  • gdT-cell: 0.5 nTPM
  • neutrophil: 0.5 nTPM
  • NK-cell: 0.5 nTPM

Brain region

  • thalamus: 2.6 nTPM
  • amygdala: 1.9 nTPM
  • medulla oblongata: 1.6 nTPM
  • pons: 1.6 nTPM
  • choroid plexus: 1.5 nTPM
  • basal ganglia: 1.4 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about CDC45.

Disease | AllUniProt

Conditions CDC45 is implicated in, by any mechanism.

Disease | GeneticClinVar

24 pathogenic / likely-pathogenic of 392 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

  • Meier-Gorlin syndrome 7

Disease | ImmuneIEDB

Conditions an epitope on CDC45 was assayed in.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.6
gnomAD pLI
0
gnomAD missense Z
0.83
DepMap mean gene effect
-2.14
DepMap dependency class
pan

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

  • CDC45
  • CDC45

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of CDC45 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads CDC45 as an antibody target. Whether an autoantibody or antibody against CDC45 could matter depends on whether native CDC45 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

CDC45 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label CDC45 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/CDC45. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

© 2026 · Seroatlas, Discover Oregon LLC · Terms of use

Loading the interactive Seroatlas protein explorer...