Seroatlas · Human Serome Atlas

RAD9A

Cell cycle checkpoint control protein RAD9A

Also known as: RAD9, RAD9A_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q99638
Gene: RAD9A
Ensembl: ENSG00000172613
Chromosome: 11
Canonical length: 391 aa
Protein class: Enzymes, Metabolic proteins, Predicted intracellular proteins
Subcellular location: Nucleoplasm,Nuclear bodies

OverviewNCBI Gene

This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair. This protein possesses 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

Canonical amino-acid sequenceUniProt

391 residues, UniProt reviewed canonical sequence.

>Q99638|RAD9A
     1  MKCLVTGGNV KVLGKAVHSL SRIGDELYLE PLEDGLSLRT VNSSRSAYAC FLFAPLFFQQ
    61  YQAATPGQDL LRCKILMKSF LSVFRSLAML EKTVEKCCIS LNGRSSRLVV QLHCKFGVRK
   121  THNLSFQDCE SLQAVFDPAS CPHMLRAPAR VLGEAVLPFS PALAEVTLGI GRGRRVILRS
   181  YHEEEADSTA KAMVTEMCLG EEDFQQLQAQ EGVAITFCLK EFRGLLSFAE SANLNLSIHF
   241  DAPGRPAIFT IKDSLLDGHF VLATLSDTDS HSQDLGSPER HQPVPQLQAH STPHPDDFAN
   301  DDIDSYMIAM ETTIGNEGSR VLPSISLSPG PQPPKSPGPH SEEEDEAEPS TVPGTPPPKK
   361  FRSLFFGSIL APVRSPQGPS PVLAEDSEGE G

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RAD9A can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.41
Highest tissue expression: 11 nTPM

Expression across tissuesHPA

Tissue

fallopian tube: 11 nTPM
skin: 11 nTPM
small intestine: 10 nTPM
duodenum: 10 nTPM
prostate: 10 nTPM
bone marrow: 9.6 nTPM

Single-cell type

epicardial cells: 459 nCPM
choroid plexus epithelial cells: 278 nCPM
cardiomyocytes: 274 nCPM
ependymal cells: 262 nCPM
respiratory ciliated cells: 255 nCPM
endometrial luminal cells: 170 nCPM

Immune cell

eosinophil: 6 nTPM
basophil: 5.3 nTPM
gdT-cell: 5.2 nTPM
memory B-cell: 4.3 nTPM
memory CD8 T-cell: 4.3 nTPM
memory CD4 T-cell: 4.2 nTPM

Brain region

hippocampal formation: 11 nTPM
midbrain: 11 nTPM
cerebral cortex: 10 nTPM
amygdala: 9.8 nTPM
medulla oblongata: 9.4 nTPM
choroid plexus: 8.9 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.05
gnomAD pLI: 0
gnomAD missense Z: -0.01
DepMap mean gene effect: -0.84
DepMap dependency class: common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of RAD9A in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads RAD9A as an antibody target. Whether an autoantibody or antibody against RAD9A could matter depends on whether native RAD9A is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

RAD9A is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label RAD9A as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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