RAD52

Protein identityUniProt · HPA

UniProt accession

P43351

Gene

RAD52

Ensembl

ENSG00000002016

Chromosome

12

Canonical length

418 aa

Protein class

Cancer-related genes, Predicted intracellular proteins

Subcellular location

Nuclear speckles,Plasma membrane

OverviewNCBI Gene

The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair. A pseudogene of this gene is present on chromosome 2. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Jul 2014]

Canonical amino-acid sequenceUniProt

418 residues, UniProt reviewed canonical sequence.

>P43351|RAD52
     1  MSGTEEAILG GRDSHPAAGG GSVLCFGQCQ YTAEEYQAIQ KALRQRLGPE YISSRMAGGG
    61  QKVCYIEGHR VINLANEMFG YNGWAHSITQ QNVDFVDLNN GKFYVGVCAF VRVQLKDGSY
   121  HEDVGYGVSE GLKSKALSLE KARKEAVTDG LKRALRSFGN ALGNCILDKD YLRSLNKLPR
   181  QLPLEVDLTK AKRQDLEPSV EEARYNSCRP NMALGHPQLQ QVTSPSRPSH AVIPADQDCS
   241  SRSLSSSAVE SEATHQRKLR QKQLQQQFRE RMEKQQVRVS TPSAEKSEAA PPAPPVTHST
   301  PVTVSEPLLE KDFLAGVTQE LIKTLEDNSE KWAVTPDAGD GVVKPSSRAD PAQTSDTLAL
   361  NNQMVTQNRT PHSVCHQKPQ AKSGSWDLQT YSADQRTTGN WESHRKSQDM KKRKYDPS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RAD52 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.54

Highest tissue expression

11 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about RAD52.

Disease | ImmuneIEDB

Conditions an epitope on RAD52 was assayed in.

• influenza T cell

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.11

gnomAD pLI

0

gnomAD missense Z

0.55

DepMap mean gene effect

-0.16

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 7% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• cellular response to oxidative stress
• DNA double-strand break processing involved in repair via single-strand annealing
• DNA recombinase assembly
• DNA recombination
• double-strand break repair
• double-strand break repair via homologous recombination
• mitotic recombination
• regulation of nucleotide-excision repair
• double-strand break repair via single-strand annealing

Molecular functions

• DNA binding
• identical protein binding
• single-stranded DNA binding

Cellular components

• nucleoplasm
• nucleus
• protein-containing complex
• protein-DNA complex

Protein domainsUniProt · Pfam · InterPro

• DNA recombination/repair protein Rad52
• DNA repair protein Rad52/59/22
• Rad52 family
• DNA repair protein Rad52/59/22 superfamily
• Rad52/22 family double-strand break repair protein

KeywordsUniProt

• DNA damage
• DNA recombination
• DNA repair
• DNA-binding
• Nucleus
• Phosphoprotein

InteractionsUniProt · HPA

Protein binding partners of RAD52 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• ABL1
• RAD51AP1
• RPA2
• SUMO1
• RAD51
• RPA3
• WRN