Seroatlas · Human Serome Atlas

TIMELESS

Protein timeless homolog

Also known as: hTIM, TIM, TIM_HUMAN, TIM1

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q9UNS1
Gene
TIMELESS
Ensembl
ENSG00000111602
Chromosome
12
Canonical length
1208 aa
Protein class
Disease related genes, Predicted intracellular proteins
Subcellular location
Nucleoplasm
Quaternary structure
Homodimer

OverviewNCBI Gene

The protein encoded by this gene is highly conserved and is involved in cell survival after damage or stress, increase in DNA polymerase epsilon activity, maintenance of telomere length, and epithelial cell morphogenesis. The encoded protein also plays a role in the circadian rhythm autoregulatory loop, interacting with the PERIOD genes (PER1, PER2, and PER3) and others to downregulate activation of PER1 by CLOCK/ARNTL. Changes in this gene or its expression may promote prostate cancer, lung cancer, breast cancer, and mental disorders. [provided by RefSeq, Feb 2014]

Canonical amino-acid sequenceUniProt

1208 residues, UniProt reviewed canonical sequence.

>Q9UNS1|TIMELESS
     1  MDLHMMNCEL LATCSALGYL EGDTYHKEPD CLESVKDLIR YLRHEDETRD VRQQLGAAQI
    61  LQSDLLPILT QHHQDKPLFD AVIRLMVNLT QPALLCFGNL PKEPSFRHHF LQVLTYLQAY
   121  KEAFASEKAF GVLSETLYEL LQLGWEERQE EDNLLIERIL LLVRNILHVP ADLDQEKKID
   181  DDASAHDQLL WAIHLSGLDD LLLFLASSSA EEQWSLHVLE IVSLMFRDQN PEQLAGVGQG
   241  RLAQERSADF AELEVLRQRE MAEKKTRALQ RGNRHSRFGG SYIVQGLKSI GERDLIFHKG
   301  LHNLRNYSSD LGKQPKKVPK RRQAARELSI QRRSALNVRL FLRDFCSEFL ENCYNRLMGS
   361  VKDHLLREKA QQHDETYYMW ALAFFMAFNR AASFRPGLVS ETLSVRTFHF IEQNLTNYYE
   421  MMLTDRKEAA SWARRMHLAL KAYQELLATV NEMDISPDEA VRESSRIIKN NIFYVMEYRE
   481  LFLALFRKFD ERCQPRSFLR DLVETTHLFL KMLERFCRSR GNLVVQNKQK KRRKKKKKVL
   541  DQAIVSGNVP SSPEEVEAVW PALAEQLQCC AQNSELSMDS VVPFDAASEV PVEEQRAEAM
   601  VRIQDCLLAG QAPQALTLLR SAREVWPEGD VFGSQDISPE EEIQLLKQIL SAPLPRQQGP
   661  EERGAEEEEE EEEEEEEELQ VVQVSEKEFN FLDYLKRFAC STVVRAYVLL LRSYQQNSAH
   721  TNHCIVKMLH RLAHDLKMEA LLFQLSVFCL FNRLLSDPAA GAYKELVTFA KYILGKFFAL
   781  AAVNQKAFVE LLFWKNTAVV REMTEGYGSL DDRSSSRRAP TWSPEEEAHL RELYLANKDV
   841  EGQDVVEAIL AHLNTVPRTR KQIIHHLVQM GLADSVKDFQ RKGTHIVLWT GDQELELQRL
   901  FEEFRDSDDV LGHIMKNITA KRSRARIVDK LLALGLVAER RELYKKRQKK LASSILPNGA
   961  ESLKDFCQED LEEEENLPEE DSEEEEEGGS EAEQVQGSLV LSNENLGQSL HQEGFSIPLL
  1021  WLQNCLIRAA DDREEDGCSQ AVPLVPLTEE NEEAMENEQF QQLLRKLGVR PPASGQETFW
  1081  RIPAKLSPTQ LRRAAASLSQ PEEEQKLQPE LQPKVPGEQG SDEEHCKEHR AQALRALLLA
  1141  HKKKAGLASP EEEDAVGKEP LKAAPKKRQL LDSDEEQEED EGRNRAPELG APGIQKKKRY
  1201  QIEDDEDD

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against TIMELESS can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.37
Highest tissue expression
21 nTPM

Expression across tissuesHPA

Tissue

  • bone marrow: 21 nTPM
  • thymus: 18 nTPM
  • tonsil: 17 nTPM
  • lymph node: 14 nTPM
  • retina: 13 nTPM
  • appendix: 10 nTPM

Single-cell type

  • retinal bipolar cells: 55 nCPM
  • monocyte progenitors: 44 nCPM
  • erythrocyte progenitors: 44 nCPM
  • megakaryocyte progenitors: 40 nCPM
  • differentiating spermatogonia: 34 nCPM
  • gonadotrophs: 32 nCPM

Immune cell

  • naive B-cell: 4.9 nTPM
  • memory B-cell: 3.3 nTPM
  • gdT-cell: 1.8 nTPM
  • intermediate monocyte: 1.5 nTPM
  • NK-cell: 1.5 nTPM
  • T-reg: 1.5 nTPM

Brain region

  • choroid plexus: 5.3 nTPM
  • thalamus: 5.2 nTPM
  • pons: 4.8 nTPM
  • medulla oblongata: 4.7 nTPM
  • white matter: 4.7 nTPM
  • midbrain: 4.5 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about TIMELESS.

Disease | AllUniProt

Conditions TIMELESS is implicated in, by any mechanism.

  • Advanced sleep phase syndrome, familial, 4 (FASPS4) MIM:620015

Disease | GeneticClinVar

1 pathogenic / likely-pathogenic of 238 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

  • Advance sleep phase syndrome, familial, 4

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.98
gnomAD pLI
0
gnomAD missense Z
0.07
DepMap mean gene effect
-1.38
DepMap dependency class
common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

  • Timeless, N-terminal
  • Timeless, PAB domain
  • Timeless
  • Timeless protein
  • Timeless-like, PAB domain
  • TIMELESS, tri-helical helix-turn-helix domain

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of TIMELESS in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads TIMELESS as an antibody target. Whether an autoantibody or antibody against TIMELESS could matter depends on whether native TIMELESS is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

TIMELESS is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label TIMELESS as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/TIMELESS. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

© 2026 · Seroatlas, Discover Oregon LLC · Terms of use

Loading the interactive Seroatlas protein explorer...