Seroatlas · Human Serome Atlas

RPA3

Replication protein A 14 kDa subunit

Also known as: REPA3, RFA3_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: P35244
Gene: RPA3
Ensembl: ENSG00000106399
Chromosome: 7
Canonical length: 121 aa
Protein class: Predicted intracellular proteins

OverviewNCBI Gene

Enables damaged DNA binding activity and single-stranded DNA binding activity. Involved in DNA metabolic process. Part of DNA replication factor A complex. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

121 residues, UniProt reviewed canonical sequence.

>P35244|RPA3
     1  MVDMMDLPRS RINAGMLAQF IDKPVCFVGR LEKIHPTGKM FILSDGEGKN GTIELMEPLD
    61  EEISGIVEVV GRVTAKATIL CTSYVQFKED SHPFDLGLYN EAVKIIHDFP QFYPLGIVQH
   121  D

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RPA3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.33
Highest tissue expression: 48 nTPM

Expression across tissuesHPA

Tissue

thymus: 48 nTPM
bone marrow: 44 nTPM
parathyroid gland: 44 nTPM
liver: 38 nTPM
choroid plexus: 37 nTPM
lymph node: 35 nTPM

Single-cell type

cardiomyocytes: 645 nCPM
gastric progenitor cells: 581 nCPM
extravillous trophoblasts: 308 nCPM
respiratory ciliated cells: 269 nCPM
fallopian tube ciliated cells: 219 nCPM
migrating cytotrophoblasts: 217 nCPM

Immune cell

naive B-cell: 77 nTPM
memory B-cell: 76 nTPM
T-reg: 69 nTPM
plasmacytoid DC: 62 nTPM
memory CD4 T-cell: 56 nTPM
non-classical monocyte: 49 nTPM

Brain region

choroid plexus: 27 nTPM
hypothalamus: 18 nTPM
white matter: 18 nTPM
pons: 17 nTPM
spinal cord: 16 nTPM
medulla oblongata: 16 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.95
gnomAD pLI: 0
gnomAD missense Z: -0.64
DepMap mean gene effect: -2.14
DepMap dependency class: pan

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 9% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

Nucleic acid-binding, OB-fold
Replication factor A protein 3
Replication factor A protein 3

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of RPA3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads RPA3 as an antibody target. Whether an autoantibody or antibody against RPA3 could matter depends on whether native RPA3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

RPA3 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label RPA3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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