Seroatlas · Human Serome Atlas

CDC27

Cell division cycle protein 27 homolog

Also known as: ANAPC3, APC3, CDC27_HUMAN, D0S1430E, D17S978E, NUC2

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: P30260
Gene: CDC27
Ensembl: ENSG00000004897
Chromosome: 17
Canonical length: 824 aa
Protein class: Cancer-related genes, Plasma proteins, Predicted intracellular proteins
Subcellular location: Nucleoplasm
Quaternary structure: Homodimer

OverviewNCBI Gene

The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of the anaphase-promoting complex (APC), which is composed of eight protein subunits and is highly conserved in eukaryotic cells. This complex catalyzes the formation of cyclin B-ubiquitin conjugate, which is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. The protein encoded by this gene and three other members of the APC complex contain tetratricopeptide (TPR) repeats, which are important for protein-protein interactions. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and it may thus be involved in controlling the timing of mitosis. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 2, 22 and Y. [provided by RefSeq, May 2014]

Canonical amino-acid sequenceUniProt

824 residues, UniProt reviewed canonical sequence.

>P30260|CDC27
     1  MTVLQEPVQA AIWQALNHYA YRDAVFLAER LYAEVHSEEA LFLLATCYYR SGKAYKAYRL
    61  LKGHSCTTPQ CKYLLAKCCV DLSKLAEGEQ ILSGGVFNKQ KSHDDIVTEF GDSACFTLSL
   121  LGHVYCKTDR LAKGSECYQK SLSLNPFLWS PFESLCEIGE KPDPDQTFKF TSLQNFSNCL
   181  PNSCTTQVPN HSLSHRQPET VLTETPQDTI ELNRLNLESS NSKYSLNTDS SVSYIDSAVI
   241  SPDTVPLGTG TSILSKQVQN KPKTGRSLLG GPAALSPLTP SFGILPLETP SPGDGSYLQN
   301  YTNTPPVIDV PSTGAPSKKS VARIGQTGTK SVFSQSGNSR EVTPILAQTQ SSGPQTSTTP
   361  QVLSPTITSP PNALPRRSSR LFTSDSSTTK ENSKKLKMKF PPKIPNRKTK SKTNKGGITQ
   421  PNINDSLEIT KLDSSIISEG KISTITPQIQ AFNLQKAAAE GLMSLLREMG KGYLALCSYN
   481  CKEAINILSH LPSHHYNTGW VLCQIGRAYF ELSEYMQAER IFSEVRRIEN YRVEGMEIYS
   541  TTLWHLQKDV ALSVLSKDLT DMDKNSPEAW CAAGNCFSLQ REHDIAIKFF QRAIQVDPNY
   601  AYAYTLLGHE FVLTEELDKA LACFRNAIRV NPRHYNAWYG LGMIYYKQEK FSLAEMHFQK
   661  ALDINPQSSV LLCHIGVVQH ALKKSEKALD TLNKAIVIDP KNPLCKFHRA SVLFANEKYK
   721  SALQELEELK QIVPKESLVY FLIGKVYKKL GQTHLALMNF SWAMDLDPKG ANNQIKEAID
   781  KRYLPDDEEP ITQEEQIMGT DESQESSMTD ADDTQLHAAE SDEF

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CDC27 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.42
Highest tissue expression: 40 nTPM

Expression across tissuesHPA

Tissue

thyroid gland: 40 nTPM
tongue: 27 nTPM
bone marrow: 26 nTPM
placenta: 24 nTPM
testis: 23 nTPM
thymus: 23 nTPM

Single-cell type

erythrocyte progenitors: 359 nCPM
vascular endothelial cells: 180 nCPM
sertoli cells: 180 nCPM
megakaryocyte progenitors: 175 nCPM
neutrophil progenitors: 171 nCPM
megakaryocyte-erythroid progenitors: 166 nCPM

Immune cell

basophil: 9.3 nTPM
NK-cell: 8.8 nTPM
T-reg: 8.4 nTPM
intermediate monocyte: 7.1 nTPM
naive B-cell: 6.3 nTPM
memory CD8 T-cell: 5.5 nTPM

Brain region

cerebellum: 53 nTPM
cerebral cortex: 46 nTPM
hypothalamus: 41 nTPM
pons: 40 nTPM
basal ganglia: 39 nTPM
midbrain: 38 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about CDC27.

Disease | GeneticClinVar

1 pathogenic / likely-pathogenic of 47 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

Pulmonary artery atresia

Disease | ImmuneIEDB

Conditions an epitope on CDC27 was assayed in.

melanoma T cell

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.27
gnomAD pLI: 1
gnomAD missense Z: 3.2
DepMap mean gene effect: -2.66
DepMap dependency class: pan

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

protein phosphatase binding

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of CDC27 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads CDC27 as an antibody target. Whether an autoantibody or antibody against CDC27 could matter depends on whether native CDC27 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

CDC27 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label CDC27 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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