Seroatlas · Human Serome Atlas

NECAB3

N-terminal EF-hand calcium-binding protein 3

Also known as: APBA2BP, dJ63M2.4, dJ63M2.5, EFCBP3, NECA3_HUMAN, NIP1, SYTIP2, XB51

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q96P71
Gene: NECAB3
Ensembl: ENSG00000125967
Chromosome: 20
Canonical length: 396 aa
Protein class: Predicted intracellular proteins
Subcellular location: Golgi apparatus
Secretome location: Intracellular and membrane

OverviewNCBI Gene

The protein encoded by this gene interacts with the amino-terminal domain of the neuron-specific X11-like protein (X11L), inhibits the association of X11L with amyloid precursor protein through a non-competitive mechanism, and abolishes the suppression of beta-amyloid production by X11L. This protein, together with X11L, may play an important role in the regulatory system of amyloid precursor protein metabolism and beta-amyloid generation. The protein is phosphorylated by NIMA-related expressed kinase 2, and localizes to the Golgi apparatus. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

396 residues, UniProt reviewed canonical sequence.

>Q96P71|NECAB3
     1  MACAGLLTVC LLRPPAPQPQ PQTPRHPQLA PDPGPAGHTL FQDVFRRADK NDDGKLSFEE
    61  FQNYFADGVL SLGELQELFS GIDGHLTDNL ETEKLCDYFS EHLGVYRPVL AALESLNRAV
   121  LAAMDATKLE YERASKVDQF VTRFLLRETV SQLQALQSSL EGASDTLEAQ AHGWRSDAES
   181  VEAQSRLCGS RRAGRRALRS VSRSSTWSPG SSDTGRSSEA EMQWRLQVNR LQELIDQLEC
   241  KVRAVGPGPH KGGPSWYPPE PGPCWRPGPH SVPSQAPRLE PLREEDLAKG PDLHILMAQR
   301  QVQVAEEGLQ DFHRALRCYV DFTGAQSHCL HVSAQKMLDG ASFTLYEFWQ DEASWRRHQQ
   361  SPGSKAFQRI LIDHLRAPDT LTTVFFPASW WIMNNN

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NECAB3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.47
Highest tissue expression: 57 nTPM

Expression across tissuesHPA

Tissue

skeletal muscle: 57 nTPM
adrenal gland: 54 nTPM
heart muscle: 43 nTPM
ovary: 40 nTPM
cerebral cortex: 38 nTPM
tongue: 30 nTPM

Single-cell type

early spermatids: 101 nCPM
late spermatids: 74 nCPM
late primary spermatocytes: 73 nCPM
parietal cells: 71 nCPM
oocytes: 60 nCPM
alveolar cells type 2: 49 nCPM

Immune cell

non-classical monocyte: 3.5 nTPM
eosinophil: 1.7 nTPM
T-reg: 1.6 nTPM
intermediate monocyte: 1.3 nTPM
classical monocyte: 1.2 nTPM
myeloid DC: 1.2 nTPM

Brain region

cerebral cortex: 64 nTPM
pons: 43 nTPM
thalamus: 41 nTPM
white matter: 38 nTPM
hippocampal formation: 38 nTPM
hypothalamus: 37 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.83
gnomAD pLI: 0
gnomAD missense Z: 0.96
DepMap mean gene effect: -0.01
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 9% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

calcium ion binding

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of NECAB3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads NECAB3 as an antibody target. Whether an autoantibody or antibody against NECAB3 could matter depends on whether native NECAB3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

NECAB3 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label NECAB3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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