LIMS1

Protein identityUniProt · HPA

UniProt accession

P48059

Gene

LIMS1

Ensembl

ENSG00000169756

Chromosome

2

Canonical length

325 aa

Protein class

Plasma proteins, Predicted intracellular proteins

Subcellular location

Focal adhesion sites,Cytosol

OverviewNCBI Gene

The protein encoded by this gene is an adaptor protein which contains five LIM domains, or double zinc fingers. The protein is likely involved in integrin signaling through its LIM domain-mediated interaction with integrin-linked kinase, found in focal adhesion plaques. It is also thought to act as a bridge linking integrin-linked kinase to NCK adaptor protein 2, which is involved in growth factor receptor kinase signaling pathways. Its localization to the periphery of spreading cells also suggests that this protein may play a role in integrin-mediated cell adhesion or spreading. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]

Canonical amino-acid sequenceUniProt

325 residues, UniProt reviewed canonical sequence.

>P48059|LIMS1
     1  MANALASATC ERCKGGFAPA EKIVNSNGEL YHEQCFVCAQ CFQQFPEGLF YEFEGRKYCE
    61  HDFQMLFAPC CHQCGEFIIG RVIKAMNNSW HPECFRCDLC QEVLADIGFV KNAGRHLCRP
   121  CHNREKARGL GKYICQKCHA IIDEQPLIFK NDPYHPDHFN CANCGKELTA DARELKGELY
   181  CLPCHDKMGV PICGACRRPI EGRVVNAMGK QWHVEHFVCA KCEKPFLGHR HYERKGLAYC
   241  ETHYNQLFGD VCFHCNRVIE GDVVSALNKA WCVNCFACST CNTKLTLKNK FVEFDMKPVC
   301  KKCYEKFPLE LKKRLKKLAE TLGRK

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against LIMS1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Cell surface

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.36

Highest tissue expression

93 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.77

gnomAD pLI

0

gnomAD missense Z

1.42

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• cell-cell adhesion
• cell-cell junction organization
• cell-matrix adhesion
• cellular response to transforming growth factor beta stimulus
• negative regulation of DNA-templated transcription
• positive regulation of canonical NF-kappaB signal transduction
• positive regulation of integrin-mediated signaling pathway
• positive regulation of substrate adhesion-dependent cell spreading
• tumor necrosis factor-mediated signaling pathway

Molecular functions

• actin binding
• protein kinase binding
• zinc ion binding

Cellular components

• cell-cell junction
• cytoplasm
• cytosol
• focal adhesion
• perinuclear region of cytoplasm
• plasma membrane

Protein domainsUniProt · Pfam · InterPro

• Zinc finger, LIM-type
• LIM and senescent cell antigen-like-containing domain protein 1-4-like
• LIMS1/2-like, LIM domain 1
• LIM and senescent cell antigen-like-containing domain protein 1/2-like
• LIM domain

KeywordsUniProt

• Acetylation
• Actin-binding
• Cell junction
• Cell membrane
• LIM domain
• Membrane
• Metal-binding
• Repeat
• Zinc

InteractionsUniProt · HPA

Protein binding partners of LIMS1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• TGFB1I1
• NCK2
• ILK
• PARVA
• RSU1