Seroatlas · Human Serome Atlas

NCKIPSD

NCK-interacting protein with SH3 domain

Also known as: AF3P21, DIP1, ORF1, SPIN90, SPN90_HUMAN, WASLBP, WISH

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q9NZQ3
Gene
NCKIPSD
Ensembl
ENSG00000213672
Chromosome
3
Canonical length
722 aa
Protein class
Disease related genes, Predicted intracellular proteins, Predicted membrane proteins
Subcellular location
Nucleoplasm,Plasma membrane

OverviewNCBI Gene

The protein encoded by this gene contains a nuclear localization signal. It plays a role in signal transduction, and may function in the maintenance of sarcomeres and in the assembly of myofibrils into sarcomeres. It also plays an important role in stress fiber formation This protein is involved in the formation and maintenance of dendritic spines, and modulates synaptic activity in neurons. The gene is involved in therapy-related leukemia by a chromosomal translocation t(3;11)(p21;q23) that involves this gene and the myeloid/lymphoid leukemia gene. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Aug 2019]

Canonical amino-acid sequenceUniProt

722 residues, UniProt reviewed canonical sequence.

>Q9NZQ3|NCKIPSD
     1  MYRALYAFRS AEPNALAFAA GETFLVLERS SAHWWLAARA RSGETGYVPP AYLRRLQGLE
    61  QDVLQAIDRA IEAVHNTAMR DGGKYSLEQR GVLQKLIHHR KETLSRRGPS ASSVAVMTSS
   121  TSDHHLDAAA ARQPNGVCRA GFERQHSLPS SEHLGADGGL YQIPLPSSQI PPQPRRAAPT
   181  TPPPPVKRRD REALMASGSG GHNTMPSGGN SVSSGSSVSS TSLDTLYTSS SPSEPGSSCS
   241  PTPPPVPRRG THTTVSQVQP PPSKASAPEP PAEEEVATGT TSASDDLEAL GTLSLGTTEE
   301  KAAAEAAVPR TIGAELMELV RRNTGLSHEL CRVAIGIIVG HIQASVPASS PVMEQVLLSL
   361  VEGKDLSMAL PSGQVCHDQQ RLEVIFADLA RRKDDAQQRS WALYEDEGVI RCYLEELLHI
   421  LTDADPEVCK KMCKRNEFES VLALVAYYQM EHRASLRLLL LKCFGAMCSL DAAIISTLVS
   481  SVLPVELARD MQTDTQDHQK LCYSALILAM VFSMGEAVPY AHYEHLGTPF AQFLLNIVED
   541  GLPLDTTEQL PDLCVNLLLA LNLHLPAADQ NVIMAALSKH ANVKIFSEKL LLLLNRGDDP
   601  VRIFKHEPQP PHSVLKFLQD VFGSPATAAI FYHTDMMALI DITVRHIADL SPGDKLRMEY
   661  LSLMHAIVRT TPYLQHRHRL PDLQAILRRI LNEEETSPQC QMDRMIVREM CKEFLVLGEA
   721  PS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NCKIPSD can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.39
Highest tissue expression
115 nTPM

Expression across tissuesHPA

Tissue

  • parathyroid gland: 115 nTPM
  • cerebral cortex: 50 nTPM
  • heart muscle: 36 nTPM
  • cerebellum: 33 nTPM
  • ovary: 30 nTPM
  • blood vessel: 29 nTPM

Single-cell type

  • platelets: 71 nCPM
  • oocytes: 69 nCPM
  • megakaryocytes: 68 nCPM
  • peritubular myoid cells: 46 nCPM
  • differentiating spermatogonia: 23 nCPM
  • pituicytes/fscs: 23 nCPM

Immune cell

  • plasmacytoid DC: 5.2 nTPM
  • myeloid DC: 3.6 nTPM
  • naive CD4 T-cell: 3.6 nTPM
  • intermediate monocyte: 3.5 nTPM
  • NK-cell: 3.4 nTPM
  • classical monocyte: 2.9 nTPM

Brain region

  • cerebral cortex: 114 nTPM
  • white matter: 75 nTPM
  • basal ganglia: 74 nTPM
  • hypothalamus: 65 nTPM
  • pons: 64 nTPM
  • hippocampal formation: 62 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.61
gnomAD pLI
0
gnomAD missense Z
0.61
DepMap mean gene effect
-0.09
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of NCKIPSD in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads NCKIPSD as an antibody target. Whether an autoantibody or antibody against NCKIPSD could matter depends on whether native NCKIPSD is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

NCKIPSD is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label NCKIPSD as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/NCKIPSD. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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