Seroatlas · Human Serome Atlas

DNAJB1

DnaJ homolog subfamily B member 1

Also known as: DNJB1_HUMAN, Hsp40, HSPF1, RSPH16B, Sis1

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: P25685
Gene: DNAJB1
Ensembl: ENSG00000132002
Chromosome: 19
Canonical length: 340 aa
Protein class: Cancer-related genes, Predicted intracellular proteins
Subcellular location: Nucleoplasm,Cytosol

OverviewNCBI Gene

This gene encodes a member of the DnaJ or Hsp40 (heat shock protein 40 kD) family of proteins. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. The encoded protein is a molecular chaperone that stimulates the ATPase activity of Hsp70 heat-shock proteins in order to promote protein folding and prevent misfolded protein aggregation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Canonical amino-acid sequenceUniProt

340 residues, UniProt reviewed canonical sequence.

>P25685|DNAJB1
     1  MGKDYYQTLG LARGASDEEI KRAYRRQALR YHPDKNKEPG AEEKFKEIAE AYDVLSDPRK
    61  REIFDRYGEE GLKGSGPSGG SGGGANGTSF SYTFHGDPHA MFAEFFGGRN PFDTFFGQRN
   121  GEEGMDIDDP FSGFPMGMGG FTNVNFGRSR SAQEPARKKQ DPPVTHDLRV SLEEIYSGCT
   181  KKMKISHKRL NPDGKSIRNE DKILTIEVKK GWKEGTKITF PKEGDQTSNN IPADIVFVLK
   241  DKPHNIFKRD GSDVIYPARI SLREALCGCT VNVPTLDGRT IPVVFKDVIR PGMRRKVPGE
   301  GLPLPKTPEK RGDLIIEFEV IFPERIPQTS RTVLEQVLPI

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DNAJB1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.4
Highest tissue expression: 441 nTPM

Expression across tissuesHPA

Tissue

esophagus: 441 nTPM
cerebellum: 295 nTPM
vagina: 279 nTPM
ovary: 271 nTPM
lung: 270 nTPM
liver: 244 nTPM

Single-cell type

esophageal apical cells: 2,851 nCPM
pancreatic duct cells: 2,411 nCPM
epididymal efferent duct absorptive cells: 1,641 nCPM
esophageal suprabasal cells: 1,116 nCPM
breast lactating cells: 959 nCPM
epididymal efferent duct ciliated cells: 952 nCPM

Immune cell

total PBMC: 322 nTPM
naive CD4 T-cell: 283 nTPM
T-reg: 272 nTPM
memory CD4 T-cell: 245 nTPM
naive CD8 T-cell: 221 nTPM
memory CD8 T-cell: 215 nTPM

Brain region

white matter: 298 nTPM
cerebellum: 288 nTPM
cerebral cortex: 251 nTPM
pons: 224 nTPM
choroid plexus: 169 nTPM
midbrain: 152 nTPM

ReferencesPubMed · IEDB

Publications for DNAJB1 from three distinct lines of evidence, kept separate because they answer different questions: whether antibodies are directed at the protein, whether a B-cell epitope has been mapped on it, and whether a T-cell epitope has. Each is labelled with its source.

Reference: AutoantibodyPubMed

4 publications

Autoantibodies to heat shock proteins 60, 70, and 90 in patients with rheumatoid arthritis.
2019 · Cell Stress Chaperones · RCR 2 · 42 citations
Autoimmunity to heat shock proteins and vitamin D status in patients with celiac disease without associated dermatitis herpetiformis.
2017 · J Steroid Biochem Mol Biol · RCR 0.8 · 20 citations
Autoantibody to heat shock protein Hsp40 in sera of lung cancer patients.
2001 · Jpn J Cancer Res · RCR 0.8 · 34 citations
Autoimmunity to heat shock protein 40 in ulcerative colitis.
2004 · J Int Med Res · RCR 0.1 · 4 citations

Sources: PubMed — antigen-level antibody evidence from a custom retrieval. Records matching a controlled set of autoantibody terms (the MeSH descriptors Autoantibodies and Autoantigens, with title and abstract term variants) were obtained through NCBI E-utilities, and their titles and abstracts parsed for constructions that direct an antibody at a named protein rather than for co-occurrence. Captured names were resolved against UniProt nomenclature and each antigen adjudicated individually against the source text. Bibliographic records from PubMed and MeSH, U.S. National Library of Medicine; citation metrics from NIH iCite (Hutchins et al., PLoS Biology 2016). Titles link to PubMed; abstracts are not reproduced here. The NLM does not endorse this analysis.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.85
gnomAD pLI: 0.04
gnomAD missense Z: 0.13
DepMap mean gene effect: -0.15
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of DNAJB1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads DNAJB1 as an antibody target. Whether an autoantibody or antibody against DNAJB1 could matter depends on whether native DNAJB1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

DNAJB1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label DNAJB1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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