HSPA4

Protein identityUniProt · HPA

UniProt accession

P34932

Gene

HSPA4

Ensembl

ENSG00000170606

Chromosome

5

Canonical length

840 aa

Protein class

Cancer-related genes, Plasma proteins, Predicted intracellular proteins

Subcellular location

Nucleoplasm,Cytosol

OverviewNCBI Gene

Predicted to enable adenyl-nucleotide exchange factor activity. Involved in chaperone-mediated protein complex assembly and protein insertion into mitochondrial outer membrane. Located in cytosol and extracellular exosome. Implicated in Chagas disease. Biomarker of chronic obstructive pulmonary disease; rheumatoid arthritis; type 2 diabetes mellitus; and ulcerative colitis. [provided by Alliance of Genome Resources, Apr 2025]

Canonical amino-acid sequenceUniProt

840 residues, UniProt reviewed canonical sequence.

>P34932|HSPA4
     1  MSVVGIDLGF QSCYVAVARA GGIETIANEY SDRCTPACIS FGPKNRSIGA AAKSQVISNA
    61  KNTVQGFKRF HGRAFSDPFV EAEKSNLAYD IVQLPTGLTG IKVTYMEEER NFTTEQVTAM
   121  LLSKLKETAE SVLKKPVVDC VVSVPCFYTD AERRSVMDAT QIAGLNCLRL MNETTAVALA
   181  YGIYKQDLPA LEEKPRNVVF VDMGHSAYQV SVCAFNRGKL KVLATAFDTT LGGRKFDEVL
   241  VNHFCEEFGK KYKLDIKSKI RALLRLSQEC EKLKKLMSAN ASDLPLSIEC FMNDVDVSGT
   301  MNRGKFLEMC NDLLARVEPP LRSVLEQTKL KKEDIYAVEI VGGATRIPAV KEKISKFFGK
   361  ELSTTLNADE AVTRGCALQC AILSPAFKVR EFSITDVVPY PISLRWNSPA EEGSSDCEVF
   421  SKNHAAPFSK VLTFYRKEPF TLEAYYSSPQ DLPYPDPAIA QFSVQKVTPQ SDGSSSKVKV
   481  KVRVNVHGIF SVSSASLVEV HKSEENEEPM ETDQNAKEEE KMQVDQEEPH VEEQQQQTPA
   541  ENKAESEEME TSQAGSKDKK MDQPPQAKKA KVKTSTVDLP IENQLLWQID REMLNLYIEN
   601  EGKMIMQDKL EKERNDAKNA VEEYVYEMRD KLSGEYEKFV SEDDRNSFTL KLEDTENWLY
   661  EDGEDQPKQV YVDKLAELKN LGQPIKIRFQ ESEERPKLFE ELGKQIQQYM KIISSFKNKE
   721  DQYDHLDAAD MTKVEKSTNE AMEWMNNKLN LQNKQSLTMD PVVKSKEIEA KIKELTSTCS
   781  PIISKPKPKV EPPKEEQKNA EQNGPVDGQG DNPGPQAAEQ GTDTAVPSDS DKKLPEMDID

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against HSPA4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.32

Highest tissue expression

85 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.24

gnomAD pLI

1

gnomAD missense Z

2.06

DepMap mean gene effect

-0.11

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• chaperone-mediated protein complex assembly
• protein folding
• protein import into mitochondrial matrix
• response to unfolded protein

Molecular functions

• adenyl-nucleotide exchange factor activity
• ATP binding
• ATP-dependent protein folding chaperone

Cellular components

• cytosol
• extracellular exosome
• nucleus

Protein domainsUniProt · Pfam · InterPro

• Heat shock protein 70 family
• Heat shock protein 70, conserved site
• Heat shock protein 70kD, peptide-binding domain superfamily
• Heat shock protein 70kD, C-terminal domain superfamily
• ATPase, nucleotide binding domain
• Hsp70 protein
• HSPA4, nucleotide-binding domain

KeywordsUniProt

• Acetylation
• ATP-binding
• Cytoplasm
• Methylation
• Nucleotide-binding
• Phosphoprotein
• Stress response

InteractionsUniProt · HPA

Protein binding partners of HSPA4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• TJP1
• BAG2
• BTF3
• CLTC
• DNAJB1
• DNAJB4
• DNAJB5
• DNAJC7
• EGFR
• HSP90AA1
• HSP90AB1
• HSPA2
• HSPA5
• HSPA8
• METTL21A
• MORF4L1
• STIP1