Seroatlas · Human Serome Atlas

DNAJB4

DnaJ homolog subfamily B member 4

Also known as: DNJB4_HUMAN, HLJ1

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9UDY4
Gene: DNAJB4
Ensembl: ENSG00000162616
Chromosome: 1
Canonical length: 337 aa
Protein class: Plasma proteins, Predicted intracellular proteins
Subcellular location: Nucleoplasm,Plasma membrane,Cytosol
Quaternary structure: Homodimer

OverviewNCBI Gene

The protein encoded by this gene is a molecular chaperone, tumor suppressor, and member of the heat shock protein-40 family. The encoded protein binds the cell adhesion protein E-cadherin and targets it to the plasma membrane. This protein also binds incorrectly folded E-cadherin and targets it for endoplasmic reticulum-associated degradation. This gene is a strong tumor suppressor for colorectal carcinoma, and downregulation of it may serve as a good biomarker for predicting patient outcomes. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015]

Canonical amino-acid sequenceUniProt

337 residues, UniProt reviewed canonical sequence.

>Q9UDY4|DNAJB4
     1  MGKDYYCILG IEKGASDEDI KKAYRKQALK FHPDKNKSPQ AEEKFKEVAE AYEVLSDPKK
    61  REIYDQFGEE GLKGGAGGTD GQGGTFRYTF HGDPHATFAA FFGGSNPFEI FFGRRMGGGR
   121  DSEEMEIDGD PFSAFGFSMN GYPRDRNSVG PSRLKQDPPV IHELRVSLEE IYSGCTKRMK
   181  ISRKRLNADG RSYRSEDKIL TIEIKKGWKE GTKITFPREG DETPNSIPAD IVFIIKDKDH
   241  PKFKRDGSNI IYTAKISLRE ALCGCSINVP TLDGRNIPMS VNDIVKPGMR RRIIGYGLPF
   301  PKNPDQRGDL LIEFEVSFPD TISSSSKEVL RKHLPAS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DNAJB4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Cell surface
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.4
Highest tissue expression: 67 nTPM

Expression across tissuesHPA

Tissue

tongue: 67 nTPM
heart muscle: 47 nTPM
adrenal gland: 47 nTPM
thyroid gland: 42 nTPM
skeletal muscle: 41 nTPM
spleen: 40 nTPM

Single-cell type

early spermatids: 114 nCPM
late spermatids: 59 nCPM
late primary spermatocytes: 33 nCPM
cardiomyocytes: 22 nCPM
pancreatic duct cells: 14 nCPM
schwann cells: 11 nCPM

Immune cell

basophil: 3.3 nTPM
naive CD4 T-cell: 3 nTPM
plasmacytoid DC: 2.3 nTPM
naive CD8 T-cell: 2.1 nTPM
T-reg: 1.9 nTPM
MAIT T-cell: 1.8 nTPM

Brain region

white matter: 53 nTPM
hypothalamus: 38 nTPM
thalamus: 35 nTPM
cerebral cortex: 35 nTPM
midbrain: 34 nTPM
cerebellum: 32 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about DNAJB4.

Disease | AllUniProt

Conditions DNAJB4 is implicated in, by any mechanism.

Congenital myopathy 21 with early respiratory failure (CMYO21) MIM:620326

Disease | GeneticClinVar

4 pathogenic / likely-pathogenic of 45 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

Congenital myopathy 21 with early respiratory failure

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.83
gnomAD pLI: 0.02
gnomAD missense Z: 1.21
DepMap mean gene effect: -0.04
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of DNAJB4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads DNAJB4 as an antibody target. Whether an autoantibody or antibody against DNAJB4 could matter depends on whether native DNAJB4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

DNAJB4 is annotated at the cell surface, where native DNAJB4 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label DNAJB4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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