HSF1
Heat shock factor protein 1
Also known as: HSF1_HUMAN, HSTF1
Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene
Protein identityUniProt · HPA
- UniProt accession
- Q00613
- Gene
- HSF1
- Ensembl
- ENSG00000185122
- Chromosome
- 8
- Canonical length
- 529 aa
- Protein class
- Cancer-related genes, Plasma proteins, Predicted intracellular proteins, Transcription factors
- Subcellular location
- Nucleoplasm,Cytosol
- Quaternary structure
- Homotrimer
OverviewNCBI Gene
The product of this gene is a transcription factor that is rapidly induced after temperature stress and binds heat shock promoter elements (HSE). This protein plays a role in the regulation of lifespan. Expression of this gene is repressed by phosphorylation, which promotes binding by heat shock protein 90. [provided by RefSeq, Jul 2017]
Canonical amino-acid sequenceUniProt
529 residues, UniProt reviewed canonical sequence.
>Q00613|HSF1
1 MDLPVGPGAA GPSNVPAFLT KLWTLVSDPD TDALICWSPS GNSFHVFDQG QFAKEVLPKY
61 FKHNNMASFV RQLNMYGFRK VVHIEQGGLV KPERDDTEFQ HPCFLRGQEQ LLENIKRKVT
121 SVSTLKSEDI KIRQDSVTKL LTDVQLMKGK QECMDSKLLA MKHENEALWR EVASLRQKHA
181 QQQKVVNKLI QFLISLVQSN RILGVKRKIP LMLNDSGSAH SMPKYSRQFS LEHVHGSGPY
241 SAPSPAYSSS SLYAPDAVAS SGPIISDITE LAPASPMASP GGSIDERPLS SSPLVRVKEE
301 PPSPPQSPRV EEASPGRPSS VDTLLSPTAL IDSILRESEP APASVTALTD ARGHTDTEGR
361 PPSPPPTSTP EKCLSVACLD KNELSDHLDA MDSNLDNLQT MLSSHGFSVD TSALLDLFSP
421 SVTVPDMSLP DLDSSLASIQ ELLSPQEPPR PPEAENSSPD SGKQLVHYTA QPLFLLDPGS
481 VDTGSNDLPV LFELGEGSYF SEGDGFAEDP TISLLTGSEP PKAKDPTVSLocalizationUniProt · AlphaFold · HPA
Whether an antibody against HSF1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.
- Antibody reachability
- Intracellular
- Secreted
- No
- Transmembrane segments
- 0
- Mean surface accessibility (rSASA)
- 0.59
- Highest tissue expression
- 127 nTPM
Expression across tissuesHPA
Tissue
- heart muscle: 127 nTPM
- skeletal muscle: 120 nTPM
- testis: 103 nTPM
- blood vessel: 67 nTPM
- colon: 67 nTPM
- pancreas: 66 nTPM
Single-cell type
- renal connecting tubule cells: 47 nCPM
- renal collecting duct intercalated cells: 46 nCPM
- renal collecting duct principal cells: 45 nCPM
- papillary tip epithelial cells: 45 nCPM
- distal convoluted tubule cells: 42 nCPM
- loop of henle epithelial cells: 36 nCPM
Immune cell
- non-classical monocyte: 32 nTPM
- eosinophil: 26 nTPM
- intermediate monocyte: 25 nTPM
- myeloid DC: 25 nTPM
- classical monocyte: 23 nTPM
- T-reg: 23 nTPM
Brain region
- medulla oblongata: 32 nTPM
- thalamus: 31 nTPM
- midbrain: 30 nTPM
- pons: 27 nTPM
- amygdala: 27 nTPM
- hypothalamus: 25 nTPM
Genetic constraint and essentialitygnomAD · DepMap
Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.
- gnomAD LOEUF (loss-of-function intolerance)
- 0.41
- gnomAD pLI
- 0.75
- gnomAD missense Z
- 0.75
- DepMap mean gene effect
- -0.28
- DepMap dependency class
- selective
Cancer expressionTCGA
Across TCGA tumor cohorts, this protein is over-expressed in roughly 20% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).
OntologyGO
Biological processes
- cellular response to angiotensin
- cellular response to cadmium ion
- cellular response to copper ion
- cellular response to diamide
- cellular response to estradiol stimulus
- cellular response to gamma radiation
- cellular response to heat
- cellular response to hydrogen peroxide
- cellular response to L-glutamine
- cellular response to lipopolysaccharide
- cellular response to potassium ion
- cellular response to sodium arsenite
- cellular response to unfolded protein
- cellular response to xenobiotic stimulus
- defense response
- DNA repair
- embryonic placenta development
- embryonic process involved in female pregnancy
- epithelial cell proliferation
- female meiotic nuclear division
- MAPK cascade
- mRNA processing
- mRNA transport
- negative regulation of cardiac muscle cell apoptotic process
- negative regulation of double-strand break repair via nonhomologous end joining
- negative regulation of epithelial cell proliferation
- negative regulation of inclusion body assembly
- negative regulation of protein-containing complex assembly
- negative regulation of transcription by RNA polymerase II
- negative regulation of tumor necrosis factor production
- positive regulation of apoptotic DNA fragmentation
- positive regulation of apoptotic signaling pathway
- positive regulation of cold-induced thermogenesis
- positive regulation of gene expression
- positive regulation of inclusion body assembly
- positive regulation of macrophage differentiation
- positive regulation of mitotic cell cycle
- positive regulation of multicellular organism growth
- positive regulation of stress granule assembly
- positive regulation of transcription by RNA polymerase II
- protein-containing complex assembly
- regulation of cellular response to heat
- regulation of transcription by RNA polymerase II
- response to activity
- response to hypobaric hypoxia
- response to nutrient
- response to peptide
- response to psychosocial stress
- response to testosterone
- spermatogenesis
- cellular response to nitroglycerin
Molecular functions
- chromatin DNA binding
- DNA binding
- DNA-binding transcription activator activity, RNA polymerase II-specific
- DNA-binding transcription factor activity
- DNA-binding transcription factor activity, RNA polymerase II-specific
- DNA-binding transcription repressor activity, RNA polymerase II-specific
- general transcription initiation factor binding
- heat shock protein binding
- Hsp90 protein binding
- identical protein binding
- promoter-specific chromatin binding
- protein heterodimerization activity
- protein kinase binding
- RNA polymerase II cis-regulatory region sequence-specific DNA binding
- RNA polymerase II intronic transcription regulatory region sequence-specific DNA binding
- sequence-specific DNA binding
- sequence-specific double-stranded DNA binding
- sequence-specific single stranded DNA binding
- STAT family protein binding
- transcription cis-regulatory region binding
- translation elongation factor binding
Cellular components
Protein domainsUniProt · Pfam · InterPro
KeywordsUniProt
InteractionsUniProt · HPA
Protein binding partners of HSF1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.
Antibody and autoantibody relevanceSeroatlas analysis
Seroatlas reads HSF1 as an antibody target. Whether an autoantibody or antibody against HSF1 could matter depends on whether native HSF1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.
HSF1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.
Annotation status
The present source text does not explicitly label HSF1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.
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