DNAJB5

Protein identityUniProt · HPA

UniProt accession

O75953

Gene

DNAJB5

Ensembl

ENSG00000137094

Chromosome

9

Canonical length

348 aa

Protein class

Predicted intracellular proteins

Subcellular location

Nucleoplasm,Cytosol

OverviewNCBI Gene

This gene encodes a member of the DNAJ heat shock protein 40 family of co-chaperone proteins. The encoded protein contains an N-terminal DNAJ domain and a C-terminal substrate binding domain but lacks the cysteine-rich domain found in other DNAJ family members. In mice, a multi-protein complex containing this protein, thioredoxin 1, and histone deacetylase 4, serves as a master negative regulator of cardiac hypertrophy. [provided by RefSeq, Mar 2017]

Canonical amino-acid sequenceUniProt

348 residues, UniProt reviewed canonical sequence.

>O75953|DNAJB5
     1  MGKDYYKILG IPSGANEDEI KKAYRKMALK YHPDKNKEPN AEEKFKEIAE AYDVLSDPKK
    61  RGLYDQYGEE GLKTGGGTSG GSSGSFHYTF HGDPHATFAS FFGGSNPFDI FFASSRSTRP
   121  FSGFDPDDMD VDEDEDPFGA FGRFGFNGLS RGPRRAPEPL YPRRKVQDPP VVHELRVSLE
   181  EIYHGSTKRM KITRRRLNPD GRTVRTEDKI LHIVIKRGWK EGTKITFPKE GDATPDNIPA
   241  DIVFVLKDKP HAHFRRDGTN VLYSALISLK EALCGCTVNI PTIDGRVIPL PCNDVIKPGT
   301  VKRLRGEGLP FPKVPTQRGD LIVEFKVRFP DRLTPQTRQI LKQHLPCS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DNAJB5 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.4

Highest tissue expression

204 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.59

gnomAD pLI

0.21

gnomAD missense Z

3.15

DepMap mean gene effect

0.04

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• negative regulation of transcription by RNA polymerase II
• protein folding
• response to unfolded protein

Molecular functions

• protein-folding chaperone binding
• unfolded protein binding

Cellular components

• cytosol
• nucleus

Protein domainsUniProt · Pfam · InterPro

• DnaJ domain
• Chaperone DnaJ, C-terminal
• HSP40/DnaJ peptide-binding
• DnaJ domain, conserved site
• Chaperone J-domain superfamily
• DnaJ homolog subfamily B
• DnaJ domain
• DnaJ C terminal domain

KeywordsUniProt

• Chaperone

InteractionsUniProt · HPA

Protein binding partners of DNAJB5 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• DNAJB1
• DNAJB4
• HSPA4
• HSPH1