Seroatlas · Human Serome Atlas

DCTN2

Dynactin subunit 2

Also known as: DCTN-50, DCTN2_HUMAN, RBP50

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q13561
Gene
DCTN2
Ensembl
ENSG00000175203
Chromosome
12
Canonical length
401 aa
Protein class
Predicted intracellular proteins
Subcellular location
Centrosome,Basal body,Cytosol,Connecting piece,Mid piece,Principal piece,End piece

OverviewNCBI Gene

This gene encodes a 50-kD subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 4-5 copies per dynactin molecule. It contains three short alpha-helical coiled-coil domains that may mediate association with self or other dynactin subunits. It may interact directly with the largest subunit (p150) of dynactin and may affix p150 in place. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2012]

Canonical amino-acid sequenceUniProt

401 residues, UniProt reviewed canonical sequence.

>Q13561|DCTN2
     1  MADPKYADLP GIARNEPDVY ETSDLPEDDQ AEFDAEELTS TSVEHIIVNP NAAYDKFKDK
    61  RVGTKGLDFS DRIGKTKRTG YESGEYEMLG EGLGVKETPQ QKYQRLLHEV QELTTEVEKI
   121  KTTVKESATE EKLTPVLLAK QLAALKQQLV ASHLEKLLGP DAAINLTDPD GALAKRLLLQ
   181  LEATKNSKGG SGGKTTGTPP DSSLVTYELH SRPEQDKFSQ AAKVAELEKR LTELETAVRC
   241  DQDAQNPLSA GLQGACLMET VELLQAKVSA LDLAVLDQVE ARLQSVLGKV NEIAKHKASV
   301  EDADTQSKVH QLYETIQRWS PIASTLPELV QRLVTIKQLH EQAMQFGQLL THLDTTQQMI
   361  ANSLKDNTTL LTQVQTTMRE NLATVEGNFA SIDERMKKLG K

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DCTN2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.55
Highest tissue expression
133 nTPM

Expression across tissuesHPA

Tissue

  • skeletal muscle: 133 nTPM
  • cerebral cortex: 109 nTPM
  • cerebellum: 107 nTPM
  • esophagus: 100 nTPM
  • tongue: 96 nTPM
  • blood vessel: 92 nTPM

Single-cell type

  • esophageal apical cells: 523 nCPM
  • late spermatids: 426 nCPM
  • esophageal suprabasal cells: 309 nCPM
  • decidual stromal cells: 221 nCPM
  • syncytiotrophoblasts: 211 nCPM
  • late primary spermatocytes: 207 nCPM

Immune cell

  • total PBMC: 63 nTPM
  • intermediate monocyte: 54 nTPM
  • non-classical monocyte: 51 nTPM
  • myeloid DC: 49 nTPM
  • classical monocyte: 49 nTPM
  • basophil: 48 nTPM

Brain region

  • pons: 100 nTPM
  • medulla oblongata: 92 nTPM
  • white matter: 87 nTPM
  • cerebellum: 86 nTPM
  • hypothalamus: 85 nTPM
  • midbrain: 81 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.25
gnomAD pLI
1
gnomAD missense Z
1.9
DepMap mean gene effect
-0.99
DepMap dependency class
common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 7% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

  • Dynamitin
  • Dynamitin

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of DCTN2 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads DCTN2 as an antibody target. Whether an autoantibody or antibody against DCTN2 could matter depends on whether native DCTN2 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

DCTN2 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label DCTN2 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/DCTN2. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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