Seroatlas · Human Serome Atlas

DCTN3

Dynactin subunit 3

Also known as: DCTN-22, DCTN3_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
O75935
Gene
DCTN3
Ensembl
ENSG00000137100
Chromosome
9
Canonical length
186 aa
Protein class
Predicted intracellular proteins
Subcellular location
Nucleoli,Cytosol,Perinuclear theca,Calyx,Mid piece,Principal piece

OverviewNCBI Gene

This gene encodes the smallest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, cytokinesis, chromosome movement, nuclear positioning, and axonogenesis. This subunit, like most other dynactin subunits, exists only as a part of the dynactin complex. It is primarily an alpha-helical protein with very little coiled coil, and binds directly to the largest subunit (p150) of dynactin. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Canonical amino-acid sequenceUniProt

186 residues, UniProt reviewed canonical sequence.

>O75935|DCTN3
     1  MAGLTDLQRL QARVEELERW VYGPGGARGS RKVADGLVKV QVALGNISSK RERVKILYKK
    61  IEDLIKYLDP EYIDRIAIPD ASKLQFILAE EQFILSQVAL LEQVNALVPM LDSAHIKAVP
   121  EHAARLQRLA QIHIQQQDQC VEITEESKAL LEEYNKTTML LSKQFVQWDE LLCQLEAATQ
   181  VKPAEE

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DCTN3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.49
Highest tissue expression
143 nTPM

Expression across tissuesHPA

Tissue

  • skeletal muscle: 143 nTPM
  • cerebral cortex: 128 nTPM
  • heart muscle: 118 nTPM
  • basal ganglia: 118 nTPM
  • amygdala: 116 nTPM
  • choroid plexus: 108 nTPM

Single-cell type

  • esophageal apical cells: 385 nCPM
  • esophageal suprabasal cells: 357 nCPM
  • esophageal basal cells: 261 nCPM
  • decidual stromal cells: 258 nCPM
  • suprabasal keratinocytes: 174 nCPM
  • hofbauer cells: 172 nCPM

Immune cell

  • basophil: 236 nTPM
  • eosinophil: 188 nTPM
  • T-reg: 180 nTPM
  • total PBMC: 153 nTPM
  • non-classical monocyte: 151 nTPM
  • intermediate monocyte: 140 nTPM

Brain region

  • cerebral cortex: 91 nTPM
  • basal ganglia: 88 nTPM
  • hypothalamus: 88 nTPM
  • pons: 86 nTPM
  • hippocampal formation: 80 nTPM
  • midbrain: 76 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.54
gnomAD pLI
0
gnomAD missense Z
0.35
DepMap mean gene effect
-0.73
DepMap dependency class
common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

  • Dynactin subunit 3
  • Dynactin subunit p22

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of DCTN3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads DCTN3 as an antibody target. Whether an autoantibody or antibody against DCTN3 could matter depends on whether native DCTN3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

DCTN3 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label DCTN3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/DCTN3. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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