Seroatlas · Human Serome Atlas

ANO6

Anoctamin-6

Also known as: ANO6_HUMAN, DKFZp313M0720, TMEM16F

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q4KMQ2
Gene
ANO6
Ensembl
ENSG00000177119
Chromosome
12
Canonical length
910 aa
Protein class
Disease related genes, Human disease related genes, Potential drug targets, Predicted membrane proteins, Transporters
Subcellular location
Plasma membrane,Cytosol
Quaternary structure
Homodimer

OverviewNCBI Gene

This gene encodes a multi-pass transmembrane protein that belongs to the anoctamin family. This protein is an essential component for the calcium-dependent exposure of phosphatidylserine on the cell surface. The scrambling of phospholipid occurs in various biological systems, such as when blood platelets are activated, they expose phosphatidylserine to trigger the clotting system. Mutations in this gene are associated with Scott syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]

Canonical amino-acid sequenceUniProt

910 residues, UniProt reviewed canonical sequence.

>Q4KMQ2|ANO6
     1  MKKMSRNVLL QMEEEEDDDD GDIVLENLGQ TIVPDLGSLE SQHDFRTPEF EEFNGKPDSL
    61  FFNDGQRRID FVLVYEDESR KETNKKGTNE KQRRKRQAYE SNLICHGLQL EATRSVLDDK
   121  LVFVKVHAPW EVLCTYAEIM HIKLPLKPND LKNRSSAFGT LNWFTKVLSV DESIIKPEQE
   181  FFTAPFEKNR MNDFYIVDRD AFFNPATRSR IVYFILSRVK YQVINNVSKF GINRLVNSGI
   241  YKAAFPLHDC KFRRQSEDPS CPNERYLLYR EWAHPRSIYK KQPLDLIRKY YGEKIGIYFA
   301  WLGYYTQMLL LAAVVGVACF LYGYLNQDNC TWSKEVCHPD IGGKIIMCPQ CDRLCPFWKL
   361  NITCESSKKL CIFDSFGTLV FAVFMGVWVT LFLEFWKRRQ AELEYEWDTV ELQQEEQARP
   421  EYEARCTHVV INEITQEEER IPFTAWGKCI RITLCASAVF FWILLIIASV IGIIVYRLSV
   481  FIVFSAKLPK NINGTDPIQK YLTPQTATSI TASIISFIII MILNTIYEKV AIMITNFELP
   541  RTQTDYENSL TMKMFLFQFV NYYSSCFYIA FFKGKFVGYP GDPVYWLGKY RNEECDPGGC
   601  LLELTTQLTI IMGGKAIWNN IQEVLLPWIM NLIGRFHRVS GSEKITPRWE QDYHLQPMGK
   661  LGLFYEYLEM IIQFGFVTLF VASFPLAPLL ALVNNILEIR VDAWKLTTQF RRLVPEKAQD
   721  IGAWQPIMQG IAILAVVTNA MIIAFTSDMI PRLVYYWSFS VPPYGDHTSY TMEGYINNTL
   781  SIFKVADFKN KSKGNPYSDL GNHTTCRYRD FRYPPGHPQE YKHNIYYWHV IAAKLAFIIV
   841  MEHVIYSVKF FISYAIPDVS KRTKSKIQRE KYLTQKLLHE NHLKDMTKNM GVIAERMIEA
   901  VDNNLRPKSE

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ANO6 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Cell surface
Secreted
No
Transmembrane segments
9
Mean surface accessibility (rSASA)
0.28
Highest tissue expression
81 nTPM

Expression across tissuesHPA

Tissue

  • adipose tissue: 81 nTPM
  • parathyroid gland: 55 nTPM
  • liver: 53 nTPM
  • ovary: 53 nTPM
  • skeletal muscle: 52 nTPM
  • small intestine: 48 nTPM

Single-cell type

  • platelets: 1,145 nCPM
  • adipocytes: 913 nCPM
  • myonuclei: 613 nCPM
  • endometrial secretory cells: 493 nCPM
  • smooth muscle cells: 434 nCPM
  • urothelial cells: 421 nCPM

Immune cell

  • non-classical monocyte: 7.1 nTPM
  • intermediate monocyte: 6.9 nTPM
  • classical monocyte: 5.9 nTPM
  • myeloid DC: 5.6 nTPM
  • total PBMC: 4.2 nTPM
  • basophil: 3.1 nTPM

Brain region

  • choroid plexus: 25 nTPM
  • hypothalamus: 18 nTPM
  • medulla oblongata: 18 nTPM
  • pons: 16 nTPM
  • spinal cord: 15 nTPM
  • white matter: 15 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about ANO6.

Disease | AllUniProt

Conditions ANO6 is implicated in, by any mechanism.

Disease | GeneticClinVar

39 pathogenic / likely-pathogenic of 421 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.14
gnomAD pLI
0
gnomAD missense Z
0.9
DepMap mean gene effect
-0.06
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of ANO6 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ANO6 as an antibody target. Whether an autoantibody or antibody against ANO6 could matter depends on whether native ANO6 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ANO6 is annotated at the cell surface, where native ANO6 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label ANO6 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/ANO6. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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