B4GALT3

Protein identityUniProt · HPA

UniProt accession

O60512

Gene

B4GALT3

Ensembl

ENSG00000158850

Chromosome

1

Canonical length

393 aa

Protein class

Enzymes, Metabolic proteins, Predicted intracellular proteins

Subcellular location

Golgi apparatus,Cytosol

OverviewNCBI Gene

This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. This gene encodes an enzyme that may be mainly involved in the synthesis of the first N-acetyllactosamine unit of poly-N-acetyllactosamine chains. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Dec 2010]

Canonical amino-acid sequenceUniProt

393 residues, UniProt reviewed canonical sequence.

>O60512|B4GALT3
     1  MLRRLLERPC TLALLVGSQL AVMMYLSLGG FRSLSALFGR DQGPTFDYSH PRDVYSNLSH
    61  LPGAPGGPPA PQGLPYCPER SPLLVGPVSV SFSPVPSLAE IVERNPRVEP GGRYRPAGCE
   121  PRSRTAIIVP HRAREHHLRL LLYHLHPFLQ RQQLAYGIYV IHQAGNGTFN RAKLLNVGVR
   181  EALRDEEWDC LFLHDVDLLP ENDHNLYVCD PRGPRHVAVA MNKFGYSLPY PQYFGGVSAL
   241  TPDQYLKMNG FPNEYWGWGG EDDDIATRVR LAGMKISRPP TSVGHYKMVK HRGDKGNEEN
   301  PHRFDLLVRT QNSWTQDGMN SLTYQLLARE LGPLYTNITA DIGTDPRGPR APSGPRYPPG
   361  SSQAFRQEML QRRPPARPGP LSTANHTALR GSH

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against B4GALT3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Other membrane

Secreted

No

Transmembrane segments

1

Mean surface accessibility (rSASA)

0.32

Highest tissue expression

33 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.64

gnomAD pLI

0.01

gnomAD missense Z

1.46

DepMap mean gene effect

-0.19

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 16% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• carbohydrate metabolic process
• galactosylceramide biosynthetic process
• glucosylceramide metabolic process

Molecular functions

• beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity
• galactosyltransferase activity
• metal ion binding
• N-acetyllactosamine synthase activity

Cellular components

• cytosol
• extracellular exosome
• Golgi apparatus
• Golgi cisterna membrane
• Golgi membrane

Protein domainsUniProt · Pfam · InterPro

• Beta-1,4-galactosyltransferase
• Galactosyltransferase, C-terminal
• Galactosyltransferase, N-terminal
• Nucleotide-diphospho-sugar transferases
• N-terminal domain of galactosyltransferase
• N-terminal region of glycosyl transferase group 7

KeywordsUniProt

• Disulfide bond
• Glycoprotein
• Glycosyltransferase
• Golgi apparatus
• Lipid metabolism
• Manganese
• Membrane
• Metal-binding
• Signal-anchor
• Transferase
• Transmembrane
• Transmembrane helix

InteractionsUniProt · HPA

Protein binding partners of B4GALT3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• CANX