Seroatlas · Human Serome Atlas

CHRNA5

Neuronal acetylcholine receptor subunit alpha-5

Also known as: ACHA5_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P30532
Gene
CHRNA5
Ensembl
ENSG00000169684
Chromosome
15
Canonical length
468 aa
Protein class
FDA approved drug targets, Predicted intracellular proteins, Predicted membrane proteins
Subcellular location
Plasma membrane,Focal adhesion sites

OverviewNCBI Gene

The protein encoded by this gene is a nicotinic acetylcholine receptor subunit and a member of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. These receptors are thought to be heteropentamers composed of separate but similar subunits. Defects in this gene have been linked to susceptibility to lung cancer type 2 (LNCR2).[provided by RefSeq, Jun 2010]

Canonical amino-acid sequenceUniProt

468 residues, UniProt reviewed canonical sequence.

>P30532|CHRNA5
     1  MAARGSGPRA LRLLLLVQLV AGRCGLAGAA GGAQRGLSEP SSIAKHEDSL LKDLFQDYER
    61  WVRPVEHLND KIKIKFGLAI SQLVDVDEKN QLMTTNVWLK QEWIDVKLRW NPDDYGGIKV
   121  IRVPSDSVWT PDIVLFDNAD GRFEGTSTKT VIRYNGTVTW TPPANYKSSC TIDVTFFPFD
   181  LQNCSMKFGS WTYDGSQVDI ILEDQDVDKR DFFDNGEWEI VSATGSKGNR TDSCCWYPYV
   241  TYSFVIKRLP LFYTLFLIIP CIGLSFLTVL VFYLPSNEGE KICLCTSVLV SLTVFLLVIE
   301  EIIPSSSKVI PLIGEYLVFT MIFVTLSIMV TVFAINIHHR SSSTHNAMAP LVRKIFLHTL
   361  PKLLCMRSHV DRYFTQKEET ESGSGPKSSR NTLEAALDSI RYITRHIMKE NDVREVVEDW
   421  KFIAQVLDRM FLWTFLFVSI VGSLGLFVPV IYKWANILIP VHIGNANK

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CHRNA5 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Cell surface
Secreted
No
Transmembrane segments
4
Mean surface accessibility (rSASA)
0.36
Highest tissue expression
4.5 nTPM

Expression across tissuesHPA

Tissue

  • duodenum: 4.5 nTPM
  • gallbladder: 4.1 nTPM
  • rectum: 4 nTPM
  • colon: 3.8 nTPM
  • skeletal muscle: 3.7 nTPM
  • testis: 2.9 nTPM

Single-cell type

  • oocytes: 175 nCPM
  • differentiating spermatogonia: 65 nCPM
  • cone photoreceptor cells: 60 nCPM
  • retinal horizontal cells: 52 nCPM
  • early primary spermatocytes: 37 nCPM
  • retinal bipolar cells: 24 nCPM

Immune cell

  • basophil: 0.9 nTPM
  • eosinophil: 0.7 nTPM
  • neutrophil: 0.7 nTPM
  • classical monocyte: 0.4 nTPM
  • intermediate monocyte: 0.4 nTPM
  • non-classical monocyte: 0.4 nTPM

Brain region

  • cerebellum: 4.2 nTPM
  • white matter: 4 nTPM
  • pons: 3.8 nTPM
  • thalamus: 3.7 nTPM
  • midbrain: 3.4 nTPM
  • cerebral cortex: 3.2 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.14
gnomAD pLI
0
gnomAD missense Z
1.07
DepMap mean gene effect
-0.08
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of CHRNA5 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads CHRNA5 as an antibody target. Whether an autoantibody or antibody against CHRNA5 could matter depends on whether native CHRNA5 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

CHRNA5 is annotated at the cell surface, where native CHRNA5 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label CHRNA5 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/CHRNA5. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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