Seroatlas · Human Serome Atlas

NCKAP1

Nck-associated protein 1

Also known as: HEM2, Nap1, NAP125, NCKP1_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q9Y2A7
Gene
NCKAP1
Ensembl
ENSG00000061676
Chromosome
2
Canonical length
1128 aa
Protein class
Predicted membrane proteins
Subcellular location
Cytosol

OverviewNCBI Gene

Contributes to small GTPase binding activity. Involved in Rac protein signal transduction; positive regulation of Arp2/3 complex-mediated actin nucleation; and positive regulation of lamellipodium assembly. Located in extracellular exosome and focal adhesion. Part of SCAR complex. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

1128 residues, UniProt reviewed canonical sequence.

>Q9Y2A7|NCKAP1
     1  MSRSVLQPSQ QKLAEKLTIL NDRGVGMLTR LYNIKKACGD PKAKPSYLID KNLESAVKFI
    61  VRKFPAVETR NNNQQLAQLQ KEKSEILKNL ALYYFTFVDV MEFKDHVCEL LNTIDVCQVF
   121  FDITVNFDLT KNYLDLIITY TTLMILLSRI EERKAIIGLY NYAHEMTHGA SDREYPRLGQ
   181  MIVDYENPLK KMMEEFVPHS KSLSDALISL QMVYPRRNLS ADQWRNAQLL SLISAPSTML
   241  NPAQSDTMPC EYLSLDAMEK WIIFGFILCH GILNTDATAL NLWKLALQSS SCLSLFRDEV
   301  FHIHKAAEDL FVNIRGYNKR INDIRECKEA AVSHAGSMHR ERRKFLRSAL KELATVLSDQ
   361  PGLLGPKALF VFMALSFARD EIIWLLRHAD NMPKKSADDF IDKHIAELIF YMEELRAHVR
   421  KYGPVMQRYY VQYLSGFDAV VLNELVQNLS VCPEDESIIM SSFVNTMTSL SVKQVEDGEV
   481  FDFRGMRLDW FRLQAYTSVS KASLGLADHR ELGKMMNTII FHTKMVDSLV EMLVETSDLS
   541  IFCFYSRAFE KMFQQCLELP SQSRYSIAFP LLCTHFMSCT HELCPEERHH IGDRSLSLCN
   601  MFLDEMAKQA RNLITDICTE QCTLSDQLLP KHCAKTISQA VNKKSKKQTG KKGEPEREKP
   661  GVESMRKNRL VVTNLDKLHT ALSELCFSIN YVPNMVVWEH TFTPREYLTS HLEIRFTKSI
   721  VGMTMYNQAT QEIAKPSELL TSVRAYMTVL QSIENYVQID ITRVFNNVLL QQTQHLDSHG
   781  EPTITSLYTN WYLETLLRQV SNGHIAYFPA MKAFVNLPTE NELTFNAEEY SDISEMRSLS
   841  ELLGPYGMKF LSESLMWHIS SQVAELKKLV VENVDVLTQM RTSFDKPDQM AALFKRLSSV
   901  DSVLKRMTII GVILSFRSLA QEALRDVLSY HIPFLVSSIE DFKDHIPRET DMKVAMNVYE
   961  LSSAAGLPCE IDPALVVALS SQKSENISPE EEYKIACLLM VFVAVSLPTL ASNVMSQYSP
  1021  AIEGHCNNIH CLAKAINQIA AALFTIHKGS IEDRLKEFLA LASSSLLKIG QETDKTTTRN
  1081  RESVYLLLDM IVQESPFLTM DLLESCFPYV LLRNAYHAVY KQSVTSSA

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NCKAP1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Cell surface
Secreted
No
Transmembrane segments
1
Mean surface accessibility (rSASA)
0.24
Highest tissue expression
84 nTPM

Expression across tissuesHPA

Tissue

  • skeletal muscle: 84 nTPM
  • parathyroid gland: 83 nTPM
  • blood vessel: 83 nTPM
  • cerebral cortex: 82 nTPM
  • tongue: 81 nTPM
  • thyroid gland: 80 nTPM

Single-cell type

  • esophageal apical cells: 450 nCPM
  • pituicytes/fscs: 336 nCPM
  • parietal cells: 292 nCPM
  • bergmann glia: 277 nCPM
  • retinal pigment epithelial cells: 273 nCPM
  • cardiomyocytes: 257 nCPM

Immune cell

  • T-reg: 0.2 nTPM
  • basophil: 0.1 nTPM
  • gdT-cell: 0.1 nTPM
  • MAIT T-cell: 0.1 nTPM
  • memory CD4 T-cell: 0.1 nTPM
  • memory CD8 T-cell: 0.1 nTPM

Brain region

  • hippocampal formation: 149 nTPM
  • cerebral cortex: 122 nTPM
  • basal ganglia: 114 nTPM
  • hypothalamus: 95 nTPM
  • midbrain: 94 nTPM
  • thalamus: 91 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about NCKAP1.

Disease | GeneticClinVar

24 pathogenic / likely-pathogenic of 232 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.04
gnomAD pLI
1
gnomAD missense Z
4.27
DepMap mean gene effect
-0.48
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of NCKAP1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads NCKAP1 as an antibody target. Whether an autoantibody or antibody against NCKAP1 could matter depends on whether native NCKAP1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

NCKAP1 is annotated at the cell surface, where native NCKAP1 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label NCKAP1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/NCKAP1. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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