GTF2A2

Protein identityUniProt · HPA

UniProt accession

P52657

Gene

GTF2A2

Ensembl

ENSG00000140307

Chromosome

15

Canonical length

109 aa

Protein class

Predicted intracellular proteins

Subcellular location

Nucleoplasm

OverviewNCBI Gene

Accurate transcription initiation on TATA-containing class II genes involves the ordered assembly of RNA polymerase II (POLR2A; MIM 180660) and the general initiation factors TFIIA, TFIIB (MIM 189963), TFIID (MIM 313650), TFIIE (MIM 189962), TFIIF (MIM 189968), TFIIG/TFIIJ, and TFIIH (MIM 189972). The first step involves recognition of the TATA element by the TATA-binding subunit (TBP; MIM 600075) and may be regulated by TFIIA, a factor that interacts with both TBP and a TBP-associated factor (TAF; MIM 600475) in TFIID. TFIIA has 2 subunits (43 and 12 kD) in yeast and 3 subunits in higher eukaryotes. In HeLa extracts, it consists of a 35-kD alpha subunit and a 19-kD beta subunit encoded by the N- and C-terminal regions of GTF2A1 (MIM 600520), respectively, and a 12-kD gamma subunit encoded by GTF2A2 (DeJong et al., 1995 [PubMed 7724559]).[supplied by OMIM, Mar 2008]

Canonical amino-acid sequenceUniProt

109 residues, UniProt reviewed canonical sequence.

>P52657|GTF2A2
     1  MAYQLYRNTT LGNSLQESLD ELIQSQQITP QLALQVLLQF DKAINAALAQ RVRNRVNFRG
    61  SLNTYRFCDN VWTFVLNDVE FREVTELIKV DKVKIVACDG KNTGSNTTE

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against GTF2A2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.42

Highest tissue expression

201 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.36

gnomAD pLI

0.03

gnomAD missense Z

0.98

DepMap mean gene effect

-1.35

DepMap dependency class

common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• positive regulation of transcription by RNA polymerase II
• positive regulation of transcription initiation by RNA polymerase II
• RNA polymerase II preinitiation complex assembly
• transcription by RNA polymerase II
• transcription initiation at RNA polymerase II promoter

Molecular functions

• protein heterodimerization activity
• protein homodimerization activity
• RNA polymerase II general transcription initiation factor activity
• RNA polymerase II general transcription initiation factor binding
• RNA polymerase II-specific DNA-binding transcription factor binding
• TBP-class protein binding

Cellular components

• cytosol
• nucleoplasm
• nucleus
• transcription factor TFIIA complex
• transcription factor TFIID complex

Protein domainsUniProt · Pfam · InterPro

• Transcription factor IIA, beta-barrel
• Transcription initiation factor IIA, gamma subunit
• Transcription factor IIA, alpha-helical domain
• Transcription initiation factor IIA, gamma subunit, C-terminal
• Transcription initiation factor IIA, gamma subunit, N-terminal
• Transcription initiation factor IIA, gamma subunit, helical domain
• Transcription initiation factor IIA, gamma subunit

KeywordsUniProt

• Host-virus interaction
• Nucleus
• Transcription
• Transcription regulation

InteractionsUniProt · HPA

Protein binding partners of GTF2A2 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• HSF1
• GTF2A1
• TBP
• TBPL1