MAPKAPK2

Protein identityUniProt · HPA

UniProt accession

P49137

Gene

MAPKAPK2

Ensembl

ENSG00000162889

Chromosome

1

Canonical length

400 aa

Protein class

Cancer-related genes, Enzymes, Predicted membrane proteins

Subcellular location

Nucleoplasm,Centrosome,Basal body

OverviewNCBI Gene

This gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

400 residues, UniProt reviewed canonical sequence.

>P49137|MAPKAPK2
     1  MLSNSQGQSP PVPFPAPAPP PQPPTPALPH PPAQPPPPPP QQFPQFHVKS GLQIKKNAII
    61  DDYKVTSQVL GLGINGKVLQ IFNKRTQEKF ALKMLQDCPK ARREVELHWR ASQCPHIVRI
   121  VDVYENLYAG RKCLLIVMEC LDGGELFSRI QDRGDQAFTE REASEIMKSI GEAIQYLHSI
   181  NIAHRDVKPE NLLYTSKRPN AILKLTDFGF AKETTSHNSL TTPCYTPYYV APEVLGPEKY
   241  DKSCDMWSLG VIMYILLCGY PPFYSNHGLA ISPGMKTRIR MGQYEFPNPE WSEVSEEVKM
   301  LIRNLLKTEP TQRMTITEFM NHPWIMQSTK VPQTPLHTSR VLKEDKERWE DVKEEMTSAL
   361  ATMRVDYEQI KIKKIEDASN PLLLKRRKKA RALEAAALAH

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MAPKAPK2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.34

Highest tissue expression

180 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.13

gnomAD pLI

1

gnomAD missense Z

3.05

DepMap mean gene effect

0.01

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 10% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• 3'-UTR-mediated mRNA stabilization
• cellular response to vascular endothelial growth factor stimulus
• DNA damage response
• inflammatory response
• inner ear development
• intracellular signal transduction
• leukotriene metabolic process
• macropinocytosis
• MAPK cascade
• p38MAPK cascade
• positive regulation of macrophage cytokine production
• positive regulation of tumor necrosis factor production
• regulation of cellular response to heat
• regulation of interleukin-6 production
• regulation of mRNA stability
• regulation of tumor necrosis factor production
• regulation of tumor necrosis factor-mediated signaling pathway
• response to cytokine
• response to lipopolysaccharide
• toll-like receptor signaling pathway
• vascular endothelial growth factor receptor signaling pathway

Molecular functions

• ATP binding
• calcium-dependent protein serine/threonine kinase activity
• calcium/calmodulin-dependent protein kinase activity
• calmodulin binding
• mitogen-activated protein kinase binding
• protein kinase activity
• protein serine kinase activity
• protein serine/threonine kinase activity

Cellular components

• centrosome
• ciliary basal body
• cytoplasm
• cytosol
• extracellular exosome
• nucleoplasm
• nucleus

Protein domainsUniProt · Pfam · InterPro

• Protein kinase domain
• Serine/threonine-protein kinase, active site
• Protein kinase-like domain superfamily
• Protein kinase, ATP binding site
• MAP kinase activated protein kinase, C-terminal
• Protein kinase domain

KeywordsUniProt

• ATP-binding
• Cytoplasm
• DNA damage
• Isopeptide bond
• Kinase
• Nucleotide-binding
• Nucleus
• Phosphoprotein
• Serine/threonine-protein kinase
• Transferase
• Ubl conjugation

InteractionsUniProt · HPA

Protein binding partners of MAPKAPK2 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• HSF1
• PHC2
• ZFP36
• HSPB1
• MAPK11
• MAPK14
• MAPKAPK3