Seroatlas · Human Serome Atlas

IRF3

Interferon regulatory factor 3

Also known as: IRF3_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q14653
Gene: IRF3
Ensembl: ENSG00000126456
Chromosome: 19
Canonical length: 427 aa
Protein class: Disease related genes, Human disease related genes, Predicted intracellular proteins, Transcription factors
Subcellular location: Cytosol
Quaternary structure: Homodimer

OverviewNCBI Gene

This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. The protein plays an important role in the innate immune response against DNA and RNA viruses. Mutations in this gene are associated with Encephalopathy, acute, infection-induced, herpes-specific, 7. [provided by RefSeq, Sep 2020]

Canonical amino-acid sequenceUniProt

427 residues, UniProt reviewed canonical sequence.

>Q14653|IRF3
     1  MGTPKPRILP WLVSQLDLGQ LEGVAWVNKS RTRFRIPWKH GLRQDAQQED FGIFQAWAEA
    61  TGAYVPGRDK PDLPTWKRNF RSALNRKEGL RLAEDRSKDP HDPHKIYEFV NSGVGDFSQP
   121  DTSPDTNGGG STSDTQEDIL DELLGNMVLA PLPDPGPPSL AVAPEPCPQP LRSPSLDNPT
   181  PFPNLGPSEN PLKRLLVPGE EWEFEVTAFY RGRQVFQQTI SCPEGLRLVG SEVGDRTLPG
   241  WPVTLPDPGM SLTDRGVMSY VRHVLSCLGG GLALWRAGQW LWAQRLGHCH TYWAVSEELL
   301  PNSGHGPDGE VPKDKEGGVF DLGPFIVDLI TFTEGSGRSP RYALWFCVGE SWPQDQPWTK
   361  RLVMVKVVPT CLRALVEMAR VGGASSLENT VDLHISNSHP LSLTSDQYKA YLQDLVEGMD
   421  FQGPGES

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against IRF3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.34
Highest tissue expression: 71 nTPM

Expression across tissuesHPA

Tissue

spleen: 71 nTPM
small intestine: 50 nTPM
prostate: 45 nTPM
kidney: 43 nTPM
ovary: 43 nTPM
thyroid gland: 42 nTPM

Single-cell type

extravillous trophoblasts: 124 nCPM
cytotrophoblasts: 95 nCPM
endometrial secretory cells: 86 nCPM
tuft cells: 81 nCPM
paneth cells: 77 nCPM
migrating cytotrophoblasts: 76 nCPM

Immune cell

T-reg: 81 nTPM
gdT-cell: 59 nTPM
memory CD4 T-cell: 57 nTPM
memory CD8 T-cell: 55 nTPM
MAIT T-cell: 53 nTPM
eosinophil: 50 nTPM

Brain region

thalamus: 18 nTPM
pons: 16 nTPM
medulla oblongata: 15 nTPM
white matter: 15 nTPM
spinal cord: 14 nTPM
amygdala: 14 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about IRF3.

Disease | AllUniProt

Conditions IRF3 is implicated in, by any mechanism.

Encephalopathy, acute, infection-induced, 7, herpes-specific (IIAE7) MIM:616532

ReferencesPubMed · IEDB

Publications for IRF3 from three distinct lines of evidence, kept separate because they answer different questions: whether antibodies are directed at the protein, whether a B-cell epitope has been mapped on it, and whether a T-cell epitope has. Each is labelled with its source.

Reference: AutoantibodyPubMed

1 publication

Regulatory role of TRIM21 in the type-I interferon pathway in Japanese encephalitis virus-infected human microglial cells.
2014 · J Neuroinflammation · RCR 2.5 · 86 citations

Sources: PubMed — antigen-level antibody evidence from a custom retrieval. Records matching a controlled set of autoantibody terms (the MeSH descriptors Autoantibodies and Autoantigens, with title and abstract term variants) were obtained through NCBI E-utilities, and their titles and abstracts parsed for constructions that direct an antibody at a named protein rather than for co-occurrence. Captured names were resolved against UniProt nomenclature and each antigen adjudicated individually against the source text. Bibliographic records from PubMed and MeSH, U.S. National Library of Medicine; citation metrics from NIH iCite (Hutchins et al., PLoS Biology 2016). Titles link to PubMed; abstracts are not reproduced here. The NLM does not endorse this analysis.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.92
gnomAD pLI: 0
gnomAD missense Z: 1.08
DepMap mean gene effect: -0.03
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of IRF3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads IRF3 as an antibody target. Whether an autoantibody or antibody against IRF3 could matter depends on whether native IRF3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

IRF3 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label IRF3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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