TRIB3
Tribbles homolog 3
Also known as: C20orf97, dJ1103G7.3, TRB3, TRIB3_HUMAN
Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene
Protein identityUniProt · HPA
- UniProt accession
- Q96RU7
- Gene
- TRIB3
- Ensembl
- ENSG00000101255
- Chromosome
- 20
- Canonical length
- 358 aa
- Protein class
- Enzymes, Predicted intracellular proteins
- Subcellular location
- Nucleoplasm
OverviewNCBI Gene
The protein encoded by this gene is a putative protein kinase that is induced by the transcription factor NF-kappaB. The encoded protein is a negative regulator of NF-kappaB and can also sensitize cells to TNF- and TRAIL-induced apoptosis. In addition, this protein can negatively regulate the cell survival serine-threonine kinase AKT1. Differential promoter usage and alternate splicing result in multiple transcript variants. [provided by RefSeq, Jul 2014]
Canonical amino-acid sequenceUniProt
358 residues, UniProt reviewed canonical sequence.
>Q96RU7|TRIB3
1 MRATPLAAPA GSLSRKKRLE LDDNLDTERP VQKRARSGPQ PRLPPCLLPL SPPTAPDRAT
61 AVATASRLGP YVLLEPEEGG RAYQALHCPT GTEYTCKVYP VQEALAVLEP YARLPPHKHV
121 ARPTEVLAGT QLLYAFFTRT HGDMHSLVRS RHRIPEPEAA VLFRQMATAL AHCHQHGLVL
181 RDLKLCRFVF ADRERKKLVL ENLEDSCVLT GPDDSLWDKH ACPAYVGPEI LSSRASYSGK
241 AADVWSLGVA LFTMLAGHYP FQDSEPVLLF GKIRRGAYAL PAGLSAPARC LVRCLLRREP
301 AERLTATGIL LHPWLRQDPM PLAPTRSHLW EAAQVVPDGL GLDEAREEEG DREVVLYGLocalizationUniProt · AlphaFold · HPA
Whether an antibody against TRIB3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.
- Antibody reachability
- Intracellular
- Secreted
- No
- Transmembrane segments
- 0
- Mean surface accessibility (rSASA)
- 0.39
- Highest tissue expression
- 81 nTPM
Expression across tissuesHPA
Tissue
- liver: 81 nTPM
- thyroid gland: 31 nTPM
- esophagus: 21 nTPM
- pancreas: 19 nTPM
- adrenal gland: 16 nTPM
- lung: 16 nTPM
Single-cell type
- esophageal apical cells: 58 nCPM
- somatotrophs: 46 nCPM
- pancreatic duct cells: 33 nCPM
- breast lactating cells: 33 nCPM
- hepatocytes: 27 nCPM
- cholangiocytes: 24 nCPM
Immune cell
- memory B-cell: 19 nTPM
- memory CD8 T-cell: 14 nTPM
- naive B-cell: 13 nTPM
- naive CD4 T-cell: 12 nTPM
- gdT-cell: 12 nTPM
- memory CD4 T-cell: 11 nTPM
Brain region
- hypothalamus: 8.4 nTPM
- choroid plexus: 7.6 nTPM
- cerebellum: 6.6 nTPM
- thalamus: 6.1 nTPM
- cerebral cortex: 6 nTPM
- basal ganglia: 5.7 nTPM
Genetic constraint and essentialitygnomAD · DepMap
Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.
- gnomAD LOEUF (loss-of-function intolerance)
- 1.89
- gnomAD pLI
- 0
- gnomAD missense Z
- -0.19
- DepMap mean gene effect
- 0.12
- DepMap dependency class
- none
Cancer expressionTCGA
Across TCGA tumor cohorts, this protein is over-expressed in roughly 7% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).
OntologyGO
Biological processes
- cellular response to insulin stimulus
- intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
- negative regulation of DNA-templated transcription
- negative regulation of fat cell differentiation
- negative regulation of fatty acid biosynthetic process
- negative regulation of insulin receptor signaling pathway
- negative regulation of MAPK cascade
- negative regulation of transcription by RNA polymerase II
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process
- positive regulation of protein ubiquitination
- regulation of autophagy
- regulation of D-glucose transmembrane transport
- regulation of MAP kinase activity
- response to endoplasmic reticulum stress
Molecular functions
- ATP binding
- mitogen-activated protein kinase kinase binding
- protein kinase binding
- protein kinase inhibitor activity
- protein serine/threonine kinase inhibitor activity
- transcription corepressor activity
- ubiquitin ligase activator activity
- ubiquitin protein ligase binding
- ubiquitin-protein transferase regulator activity
Cellular components
Protein domainsUniProt · Pfam · InterPro
KeywordsUniProt
InteractionsUniProt · HPA
Protein binding partners of TRIB3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.
Antibody and autoantibody relevanceSeroatlas analysis
Seroatlas reads TRIB3 as an antibody target. Whether an autoantibody or antibody against TRIB3 could matter depends on whether native TRIB3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.
TRIB3 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.
Annotation status
The present source text does not explicitly label TRIB3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.
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