DAPK2

Protein identityUniProt · HPA

UniProt accession

Q9UIK4

Gene

DAPK2

Ensembl

ENSG00000035664

Chromosome

15

Canonical length

370 aa

Protein class

Enzymes, Predicted intracellular proteins, Predicted membrane proteins

Subcellular location

Golgi apparatus,Vesicles

Quaternary structure

Homodimer

OverviewNCBI Gene

This gene encodes a protein that belongs to the serine/threonine protein kinase family. This protein contains a N-terminal protein kinase domain followed by a conserved calmodulin-binding domain with significant similarity to that of death-associated protein kinase 1 (DAPK1), a positive regulator of programmed cell death. Overexpression of this gene was shown to induce cell apoptosis. It uses multiple polyadenylation sites. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

370 residues, UniProt reviewed canonical sequence.

>Q9UIK4|DAPK2
     1  MFQASMRSPN MEPFKQQKVE DFYDIGEELG SGQFAIVKKC REKSTGLEYA AKFIKKRQSR
    61  ASRRGVSREE IEREVSILRQ VLHHNVITLH DVYENRTDVV LILELVSGGE LFDFLAQKES
   121  LSEEEATSFI KQILDGVNYL HTKKIAHFDL KPENIMLLDK NIPIPHIKLI DFGLAHEIED
   181  GVEFKNIFGT PEFVAPEIVN YEPLGLEADM WSIGVITYIL LSGASPFLGD TKQETLANIT
   241  AVSYDFDEEF FSQTSELAKD FIRKLLVKET RKRLTIQEAL RHPWITPVDN QQAMVRRESV
   301  VNLENFRKQY VRRRWKLSFS IVSLCNHLTR SLMKKVHLRP DEDLRNCESD TEEDIARRKA
   361  LHPRRRSSTS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DAPK2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.32

Highest tissue expression

45 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.28

gnomAD pLI

0

gnomAD missense Z

0.35

DepMap mean gene effect

-0.06

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• anoikis
• apoptotic process
• intracellular signal transduction
• neutrophil migration
• positive regulation of apoptotic process
• positive regulation of neutrophil chemotaxis
• protein autophosphorylation
• protein phosphorylation
• regulation of apoptotic process
• regulation of autophagy
• regulation of intrinsic apoptotic signaling pathway
• positive regulation of eosinophil chemotaxis

Molecular functions

• ATP binding
• calmodulin binding
• identical protein binding
• protein serine kinase activity
• protein serine/threonine kinase activity

Cellular components

• cytoplasm
• cytoplasmic vesicle
• Golgi apparatus
• nucleus
• autophagosome lumen

Protein domainsUniProt · Pfam · InterPro

• Protein kinase domain
• Serine/threonine-protein kinase, active site
• Protein kinase-like domain superfamily
• Protein kinase, ATP binding site
• Protein kinase domain

KeywordsUniProt

• Apoptosis
• ATP-binding
• Calmodulin-binding
• Cytoplasm
• Cytoplasmic vesicle
• Kinase
• Nucleotide-binding
• Phosphoprotein
• Serine/threonine-protein kinase
• Transferase

InteractionsUniProt · HPA

Protein binding partners of DAPK2 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• SFN
• YWHAE
• YWHAG
• YWHAH
• YWHAQ
• YWHAZ
• ATF4